The Inducing effect of lemon juice in the total antioxidants capacity, male hormones in New Zealand white male rabbits fattened experimentally
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Abstract
This study is designed to estimate the effect of experimental fattening of New Zealand white male rabbits and then use lemon juice to show their impact on the total antioxidants capacity (TAC) and the level of the Spermatogenic Stimulating Hormone(SSH) and Interstitial Cell Stimulating Hormone (ICSH) and testosterone. Used 40 male from New Zealand white rabbits and ages ranged from 10.8 months and weights ranging from 1400-1250gm, divided into two groups of 20 rabbit/ group and putted in separate cages. Fed the first group to standard diet, while the second group fed special diet rich in fat for the purpose of fattening for a period of 12 weeks after a period all rabbits were weighted, and divided the groups into four groups of 10 rabbit / group, are control group:, lemon Group:, fattening group and fattening with lemon Group, all these groups underwent standard condition of water and diet, lemon group and Fattening with lemon group dosing with 4 ml/k b.wt, Lemon juice for 8 weeks where a daily dosage. The results showed that treatment with lemon juice has led to a significant increase in the level of (P≤0.0001) in each of the TAC and the level of hormones in each of lemon Group and fattening with lemon group as compared to the control group and fattening group respectively, while the significant decrease in the level of (P≤0.0001) in (P≤0.0001) in the TAC and the concentration of hormones in fattening group compared to the control group.
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References
1- Jinabhai ,C.C.; Reddy, P.; Taylor, M.; MongeKi, D.; Kamabaran, N.; Omardien, R. and Sullivan, K.R. Sex differences in under and over nutrition among school going Black teenagers in South Africa: an uneven nutrition trajectory. Trop. Med. Int. Health. 12(2007)8:944–952.
2- WHO. Consultation on Obesity: Preventing and Managing the Global Epidemic. Geneva, Switzerland: World Health Organization; WHO Technical Report Series 894(2000).
3- Ali, A.T.; and Crowther, N.J. Factors predisposing to obesity: a review of the literature .JEMDSA. 14 (2009) 2:81-84.
4- Field, A. E.; Coakley, E. H.; Must, A.; Spadano, J. L.; Laird, N.; Dietz, W. H.; Rimm, E. and Colditz, G.A. Impact of overweight on the risk of developing common chronic diseases during a 10- year period .Arch. Intern. Med.161(2001) 13:1581-1586.
5- Mokdad, A. H.; Ford, E. S.; BoWman, B. A.; Ditz, W. H.; Vinicor, F.; Bales, V. S. and Marks, J. S. Prevalence of obesity, diabetes, and obesity-related health risk factors, 2001. JAMA. 289(2003). (1):76-79.
6- Anonymous. Wealth of India: Adictionary of Indian raw materials and industrial products . (1988). Mudran Enterprises New Delhi, India. Vol-II. PP: 188-208.
7- Sidana, J.; Saini, V.; Dahiya, S.; Nain, P. and Bala, S. A Review on Citrus – ―The Boon of Nature‖.Int. J. Pharm. Sci. Rev. Res.18 (2013) 2:20-27.
8- Marín, F.R.; Martinez, M.; Uribesalgo, T.; Castillo, S. and Frutos, M.J. Changes in nutraceutical composition of lemon juices according to different industrial extraction systems. Food Chem. 78(2002)3:319- 324.
9- Bellocco, E.; Caristi, C.; Panzera, V.; Toscano, G.; Vadalà, V. and Leuzzi, U. Flavonoids detection by HPLC-DAD-MS-MS in lemon juices from Sicilian cultivars. J. Agric. Food Chem. 51(2003)12: 3528-3534.
10- Ranganna, S.; Govindarajan, V.S. and Ramana, K.V. Citrus fruits—varieties, chemistry, technology and quality evaluation. Critical Rev. Food Sci. Nutr. 18(1983)4: 313-386.
11- Chevallier, A. Encyclopedia of Medicinal Plants. (1996). New York, NY, DK Publishing, PP: 81.
12- Touhami, M.; Laroubi, A.; Elhabazi, K.; Loubna, F.; Zrara, I.; Eljahiri, Y.; Oussama, A.; Grases, F. and Chait, A. Lemon juice has protective activity in
rat’s urolitthiasis model. BMC Urology, 7(2007)18:1-10.
13- Sapkota, R.; Dasgupta, R.; Nancy, D. and Rawat, S. Antibacterial effects Plants extracts on human microbial pathogens & microbial limint tests .IJRPC 2(2012) (4):926-936.
14- Riaz , A. and Khan , R.A. Effect of combination doses of Citrus limon and Punica granatum juice on blood parameters in rabbits. IJRPC. 3(2013)4:1-14.
15- Tajoda, H.N.; Kurian, J.C. and Bredenkamp, M.B. Reduction of Cholesterol and Triglycerides in Volunteers using Lemon and Apple. IJHSS. 3(2013)18:60-64.
16- Cheij, R. McDonald Encyclopedia of Medical Plant. (1984). Ltd. McDonald and Co. London, PP:
209, 309, 313.
18- Dharmaraj, B.; Srivastava, R.K. and Ghalaut , V. Influence of Liraglutide Alone and in Combination With Glimepiride on Body Weight in Obese-Diabetic Rabbits. J. Pharma. Innov. 2(2013)7: 52-57.
19- Steel, R. G. D. and Torrie, J. H. Principles and Procedures of Statistics.2thed. (1980).New York. McGrow-Hill Book. Company. Inc. PP: 481.
20- SAS. SAS / STAT Users Guide for Personal Computers. (2001). Release 6:12. SAS Institute Inc. Cary, N. C., USA.
21- Pietta, P.G. Flavonoids as antioxidants. J. Nat. Prod.63(2000). 7: 1035–1042.
22- Bruno, R.S.; Leonard , S.W. and Atkinson, J. Faster plasma vitamin E disappearance in smokers is normalized by vitamin C supplementation. Free Rad. Biol. Med. 40(2006)4:689-697.
23- Olukanni, O.D.; Akande, O.T. ; Alagbe, Y.O.; Adeyemi, S.O.; Olukanni, A.T. and Daramola, G.G. Lemon Juice Elevated Level of Reduced Glutathione and Improved Lipid Profile in Wistar Rats. Eurasian J. Agric. & Environ. Sci.13(2013)9:1246-1251.
24- Kim, S.S.; Gallaher, D.D. and Csallany, A.S. Vitamin E and probucol reduce urinary lipophilic aldehydes and renal enlargement in streptozotocin-induced diabetic rats. Lipids, 35(2000). (11): 1225–1237.
25- Nan, L.; Kehui, O.; Jiaoying, C.; Wuying, Y. and Wenjun, W. Research on Anti-oxidant Activity and
Hypolipemic Mechanism of Aloes Flavonoids in Mice. J. Food and Nutr. Res. 2(2014)9: 601-607.
26- Farshad, A.; Fereydoun, S.; Mahmoud, D. and Abbas, R.F. Assessment of antioxidant enzyme activities in erythrocytes of pre-hypertensive and hypertensive women. JRMS. 15 (2010)5: 270-278.
27- Sfar, S.; Boussoffara, R.; Sfar, M and Kerkeni, A. Antioxidant enzymes activities in obese Tunisian Children . Nutr. J. 12(2013). (18): 2-7.
28- Novero, R.P.; Beck, M.M.; Gleaves, E.W.; Johason, A.L. and Deshazer, J.A. Plasma progesterone, luteinizing hormone concentrations and Granulose cell responsiveness in heat stressed hens. Poultry Sci. 70(1991). (11): 2335-2339.
29- Abdel Moneim, A.E. Citrus peel extract attenuates acute cyanide poisoning-induced seizures and oxidative stress in rats. CNS Neurol. Disord. Drug Targets. 13(2014). (4):638-646.
30- Yoshioka, M.; Imanag, M. and Ueyama, H. Maximum tolerable dose of red pepper decreases fat intake independently of spicy sensation in the mouth. Br. J. Nutr. 91(2004). (6):991-995.
31- Hong, M.Y.; Seeram, N.P. and Heber, D. Pomegranate polyphenols down-regulate expression of androgen-synthesizing genes in human prostate cancer cells overexpressing the androgen receptor. J. Nutr. Biochem. 19(2008). (12):848-855.
32- Zhou, C.Y.; Ran, L. and Zhang, X. The effects on the serum lipids, fatty liver and lipid peroxidation of vitamin E in experimental hyperlipemia rats.China Clin. Pharmacol. Therapeut. 9(2004).: 202-215.
33- Onody, A.; Csonka, C.; Giricz, Z. and Ferdinandy, P. Hyperlipidemia induced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts. Cardiov. Res. 58(2003). (3): 663-670.
34- Hamzaa, R.G.; El Shahat, A.N. and Mekawey, H.M.S. Fruits in Ameliorating the Oxidative Stress Induced in γ-Irradiated Male Rats. Biochem. Anal. Biochem., 1(2012). (8):2-6.
35- Jeong, H.J.; Shin, Y.G.; Kim, I.H. and Pezzuto, J.M. Inhibition of aromatase activity by flavonoids. Arch. Pharm. Res . 22(1999).:309-312
36- Pelusi, C and Pasquali, R. The Significance of Low Testosterone Levels in Obese Men. Curr. Obes. Rep. 1(2012). (4):181–190.
37- Loves, S.; Ruinemans-Koerts, J. and de Boer, H. Letrozole once a week normalizes serum testosterone in obesity-related male hypogonadism. Eur. J. Endocrinol. 158(2008).: 741–747.
38- Pitteloud, N.; Hardin, M.; Dwyer, A.A.; Valassi, E.; Yialamas, M.;Elahi, D. and Hayes, F.J. Increasing insulin resistance is associated with a decrease in Leydig cell testosterone secretion in men. J. Clin. End. Met. 90(2005). (5):2636–2641.
39- Soyupek, S.; Armağan, A.; Serel, T.A.; Hoşcan, M.B.; Perk, H.; Karaöz, E. and Candir, O. Leptin expression in the testicular tissue of fertile and infertile men. Arch. Androl. 51(2005). (3):239–246.
40- Wu, N. and Murono, E.P. Temperature and germ cell regulation of leydig cell proliferation stimulated by sertoli cell secreated mitogenic factor :apossible role in cryptorchidism. Andrologia, 28(1996). (5): 247-257.
41- Ishihura, M.; Itoh, M.; Miyamoto, K.; Suna, S.; Takenchi, Y.; Takenaka, I. and Jitsunar., F. Spermatogenic disturbance induced by di (2-ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat. Int. J. Androl. 23(2000). (2):85-94.
42- Volek, J.; Gomez, A.; Love, D.; Avery, N.; Sharman, M. and Kraemer, W . Effects of a high-fat diet on postabsorptive and postprandial testosterone responses to a fat-rich meal. Metabolism, 50(2001). (11): 1351-1355.