Isolation and Identification of Bacteriodes Fragilis and Determination of the Synergest Effect of Some Antibiotics Used in Treatment Patients Infected with this Bacteria in Samarra Hosiptal
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Abstract
The study dealts with isolating and identifying of B.fragilis from patients entered and visited the state hospital in samarra from 1/9/2013 to 1/5/2014. (150) samples were collected and the patients᾽ samples are distributed into (100) samples taken from the patients having diarrhea and (50) samples taken from the femal patients having complication followed the female surgical operations and the vaginal inflammation.
The results show that only (5) samples from all the diarrhea samples develop positive bacteriological growth by B. fragilis at ratio (5%). This study demonstrated that the new-born infants and children who are less than 5 years are the most exposed to diarrhea caused by B. fragilis that the ratio of the infection is (7%) (11%) respectively, whereas the results for the smears taken from the female patients having complication followed the female surgical operations and the vaginal inflammation show growth to B. fragilis in (3) samples and at ratio (6%), it is evident that the adult group of the women ranged between (14-20) years are the most exposed to the vaginal inflammations and the complication that followed the femal surgical operation.
It also tests the susceptibility of the bacterial isolates aginst (17) types of the antibiotic. The results appear that the B. fragilis bacteria are extremely resistant to numerous antibiotics, so it appears a ratio of resistance to antibiotics (PRL), (AM), (P), (CTX), (AK), (TOB), (V), and (Eh). Their ratios are reaches (100%, 100%, 75%, 62%, 87%, 62%, 87%, 75%) respectively, whereas the B. fragilis are show a highly susceptibility aginst the antibiotics (C), (M), (DA), (AX), (IMP) and (G), so the ratio of their susceptibility reaches to (100%) in each type of these antibiotics.
It also calculats the minimum inhibitory concentration (MIC) for the antibiotics: (M), (AM), (G), (CIP), (CTX) , (Eh) and (G). It is also tests the synergical activity for these antibiotics aginst the isolates under-studied, which proves that the most effective synergies against B.fragilis is the synergy between (C) and (CTX) at ratio (60%) than the other isolates group. The synergy between (M) and( Rif) is shows an evident effect aginst the under-studied isolates with ratio (75%) from all the resistant isolates.
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References
2- Shoemaker, N. B.; Vlamakis, H.; Hayes, K. and Salyers, A. A. (2001). Evidence for extensive resistance gene transfer among Bacteroides spp. and among Bacteroides and other genera in the human colon. Appl. Environ. Microbiol. 67:561–568.
3- Bergers, S. (2014). Gidenon Guide to Medically Important Bacteria. Gideon, Inc. ISBN: 1617558427.
4- Hartmeyer, G. N.; Soki, J.; Nagy, E. and Justesen, U.S. (2012). Mult -idrug resistant Bacteroides fragilis group on the rise in Europe. Journal of Medical Microbiology. 61: 1784-1788.
5- Besty, T. and Keogh, J. (2005). Microbiology Demystified. The Mc-Graw-Hill Companies.
6- Snydman, D .R .; Jacobus, N. V.; Mc Dermott, L. A.; Ruthazer, R . et al. (2011) .Lessons learned from the anaerobe survey: historical prerespective and review of the most recent data (2005-2007). Clin. Infect .Dis .50:s26_s33. PMID: 20067390. DOI: 10.1086/647940.
7- Brook, I. (1984). Synergistic combinations of antimicrobial agents against anaerobic bacteria. The pediatric infectious disease journal. V6.
8- Ralph, E. D. and Amatneks, Y. (1980). Potentially synergistic antimicrobial combinations with Metronidazole aginst Bacteroides fragilis. Antimicrobial agents and chemotherapy. P. 379-382.
9- Whiting, J. L.; Chang, N.; Chow, A. W. (1987). Interaction of ciprofloxacin with clindamycin, mitronidazole, cefoxitin, cefotaxime, and mezlocillin against gram–positive and gram-negative anaerobic bacteria. Antimicrob. Agents. Chemother, 31(9): 1379.
10- CLSI. clinical and laboratory standards institute. (2011). Methods for antimicrobial susceptibility testing of anaerobic bacteria Approved standard M11-
A7. Clinical and Laboratory Standards Institute, Wayne, Pa.
11- Jubran, S. (1986). Isolation and Identification of Bacteria from Traumatic Wound and their Senstivity Pattren of Antibiotic. Athesis of M. S. Degree in Bacteriology AL-mostansirya University.
12- Mayers, L. L.; Shoop, D. S.; Stackhouse, L. L.; Newman, F. S.; Flaherty, R. J.; Leston, G. W. and Sack, R. B. (1987). Isolation of enterotoxigenic Bacteroides fragilis from humans with diarrhea. J. Clin. Microbiol. 25(12): 22330.
13- Selkon, J. B. (1981). The bacteriology of war wound. J. Roy. Naval Med serv,67:160-164·
14- Brooks, G.; Carroll, K. C.; Butel, J.; Morse, S. (2012). Jawetz Melnick, and Adelbergs Medical Microbiology. 2thed. Mc Graw Hill Professional.
15- Ramamarthy, D.; Pazhani, G.; Sarker, A.; Nandy, K. R.; Rajendran, K.; Sur, D. and Ramammurthy, T. (2013). Case Control study on the Role of Entertoxigenic Bacterooides fragilis as acase of diarrhea among children in Kalkata, India. DOI: 10 . 173/1/Journal.
16- Yehya, M.; Hamze, M.; Mallat, H. and Dabbousi, F. ( 2013). Prevalence and antibiotic susceptibility of Bacteroides fragilis group isolated from stool samples in north Lebanon. Braz. J. Microbiol. Vol.44.No,35 aopaulo , July/ sept.
17- Klosterman, L. (2009). Immune system (The amazing human body). Marshal Cavendish Corporation. New York. Tarytown.
18- Pop. M.; Walker, W. A.; Paulson, J.; Lindsay, B.; Antonio, M.; et. al. (2014). Diarrhea in young children from low-income countries leads to larg- scale alterations in intestinal microbita. Compostion genome biology. DOI. 101186.
19- Danmd, U. and Sjmd, C. (2003): After cesearn section in the rural origin in the osloe; Department of obstetrics and Gynocology; Arnold balmer Childrean and Woman Hospital in the Floredia , 20(2): 63-67 ·
20- Shiryaev, A. S.; Remacle, G. A.; Cherno, V. A.; et.al.(2013). Substrate cleavage profiling suggest adistinct function of Bacteroides fragilis metallo-proteinases (Fragilysin and Metalloproteinase II) at the microbiome inflammation. Cancer Interface. clinical microbiology and infection diseases.
21- Jamal, W.; AL-Hashem, G. and Rotimi, O. V.(2014). Antimicrobial resistance among anaerobes isolate from clinical specimens in Kuwait hospitals : comparative analysis of year data. Anaerobes. Antibiotic, Resistance, Kuwait.
22- Nakano, V.; Silva, A. D. N.; Rafael, V.; Marino, C.; Wexler, H. M. and Avila-Compos, M. J. (2011). Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains. Clinics, Vol.
66, No.4.
23- Fong. W. I. and Karl-Darlica, A. (2008). Antimicrobia resistance and implications for the 21 st Century. Springer Science and Business Media, LLC. ISBN: 978-0-387-72417-1. P208-225.
25- Akhi, M. T.; Chotaslou, R.; Shirinzadeh, M.; Pirzadeh, T.; Behazad, N. M. (2013b). Comparision of E-test and disk diffusion test for antibiotic resistance testing of enterotoxigenic and non-enterotoxigenic Bacteroides fragilis isolated from stool. Jundishapur Journal of Microbiology. 6(5): e9800.
23- Pauer, H.; Cavalcanti, V. N. S.; Teixeira, L. F.; Santos-Filho, J.; Vommaro, C, R.; Poliveira, C. S. C. A.; Ferreira, O. E. and Domingues, S. P. C. M. R.(2013). Inactivation of fibronectin binding Ton B_dependent protein increases adhesion properties of Bacteroides fragilis. Jurnal Medical Microbiology. Vol,62. No.1, pt10: 1524-1530.
29- Talan, T. (2000). Comparison of ciprofloxacin (7days) and Trimethoprime - sulfmethoxazole (14days), uncomplicated pyelonephrits in women: arandomized tiral. JAMA. 283: 1583-1590.
30- Prescott, L. M.; Harley, J. P. and Klein, D.A. (2005). Microbiology. W.M. C. Brown publisher, New York .
31- Stiefel, U.; Nerandzic, M. M.; Pultz, J. M. and Ponskey, J. C. (2014). Gastrointestinal colonization with a cephalosporinase-producing Bacteroides species preserves colonization resistance agents vancomycin–risistance Enterococcus and Clostridiam difficile in cephalosporin treated mice. DOI: 10. 1128/AAC. 02782-14.
33- Fille, M., Mango, M., Lechner, M. and Schumann, R. (2006). Bacteroides fragilis group: tends in resistance. Curr. Microbiol. 52: 153-157.
34- Wang, D. F.; Liao, H. C.; Lin, T. Y.; Sheng, H. W. and Hsueh, R. P. (2014). Trends in the susceptibility of commonly encountered clinicly significant anaerobes to 16 antimicrobial agents, including fidaxomicin and rifaximin, Northern-Tawin. J. Clin. Microbial infect Dis. 33:2041-2052.
35- Karlowsky, A. J.; Walkty, J. A.; Adam, J. H.; Baxter, R. M.; Hoban, J. D. and Zhanel, G. G. (2011). Prevalence of antimicrobial resistance among clinical isolates of Bacteroides fragilis group in Canada in 2010-2011: CANWARD surveillance study.
Antimicrob. Agents Chemother. DOI; 10. 1128/ AAC. 05823-11.
36- Meggersee, R. L. (2014). Metronidazole resistance in clinical Bacteroides fragilis isolates from grooteschur hospital, Cape Town, South Africa. Dept of Molecullar Cell Biology.
37- Galvao, B. P.; Meggersee. R. L. and Abratt, V. R. (2011). Antibiotic resistance and adhesion potential Bacteroides fragilis clinical isolates from Cape–Town, South Africa. Anaerobe. 17(4): 142-6.
39- Franch, GL.; Shanon, KP. and Simmons, N. (1996). Hospital outbreak of Klebsiella pneumonia resistant to broad-spectrum cephalosporins and beta-lactam, beta-lactamase inhibition combinations hyper production of SHV-5beta lactamase. J. Clin. Microbiol, 34(2):p 358-363.
40- Haser, A. R. (2013). Antibiotic basics for clinicaians, The ABC of choosing the right antibacterial agent. 2nded. Lippincott willians and wilkins. Baltemere. Pp 87-88.
41- Choudhary, M. and Payasi, A. (2014). Emerging Metronidazole Resistance in Anaerobes and Mapping their Susceptibility Behaviour. American Journal of Infectious Diseases.10(2): 56-63.
42- Goldberg, N. D.; Valdlamudi, A. and Parrish, N.(2015). Treatment of Refractory Crohns Disease and Pyoderma Gangrenosm with a Combination Regimen of Rifaximin, Gentamycin and Mitronidaz -ole. Case Rep Gastroenterol, 9:25-28.