The Use Of Polymerase Chain Reaction In Real Time To Diagnose The Tuberculosis (TB) for patient from Erbil
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Abstract
were examined sample 30 patients (male 19 and female 11) suspected clinically infected with tb and using RT-PCR the sample from sputum and ICT Assay from blood plasma were ratios 36%, 13% respectively. and test values were Chi square from diagnose tb from sputum by RT-PCR for female 3.798n.s, P-Value 0.0571 and male were Chi square 3.185n.s , P-Value0.00714 non significant difference among females and male but the value of male closer to significant. test values were chi square from diagnose tb from blood plasma by ICT for female 3.157n.s, P-Value 0.1782 and male were chi square 1. 831n.s , P-Value 0.381 non significant difference among females and male but the value of female closer to significant. diagnose tb from urine by RT-PCR dose not appear any result. Results showed despite the lack of significant differences between the ways studied but the RT-PCR offers a way sensitive, efficient and accurate for the diagnosis and determine tb infection especially from sputum and followed ICT test less efficient and indexation DNA of tb from urin by RT-PCR is non efficient .
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