A comparative study impact of real estate Tenordin and Captosam on the activity of alkaline phosphatase enzyme in patients with chronic high blood pressure
Article Sidebar
Main Article Content
Abstract
A study was conducted on 64 serum samples of blood from people [36 male and 28 female] aged between 48-65 years old, 47 of whom suffer from chronic high blood pressure, and divided the patient samples to the first group using the drug Tenordin, and a second set using drug Captosam, and 17 healthy sample 9 male and 8 Female control group for comparison.
The results showed a significant increase in the activity of the alkaline phosphatase ALP for the first group, 19.44±2.94 Kind and King/100ml compared with control group 14.24±2.14 Kind and King/100ml. The second group showed a significant decrease enzyme activity, amounting to 7.16 ± 1.90 Kind and King/100ml compared with the control group.
Three different concentrations of drugs Captosam and Tenordin were prepared and studied their impact on the effectiveness of the enzyme ALP in vitro, and results showed that the drug Tenordin shows a strong activating effect, but the drug Captosam showed remarkable inhibition effect. Also studied the kinetics of the effect of a drug Captosam inhibitor on the activity of the enzyme and the results showed that the value of Km is 6.8965 mmol /L, while the value of Vmax are 12.5 mmol /L min., as results showed when studying the inhibition of the low values of Km and Vmax, as the value of Km is 3.077 mmol /L, while the Vmax value was 5.555 mmol /L min., and shows that the inhibition type is Uncompetitive.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
[2] Thomas, W. and Rowland, D. Pediatric laboratory exercise testing clinical guideline Human kinetics. 1993, 57-59.
[4] Hypertension Diagnosis and Treatment. Institute for Clinical Systems Improvement, 2014.
[5] Kikuya, M.; Hansen, T.W.; Thijs, L. et al. On behalf of the International Database on Ambulatory blood pressure monitoring in relation to cardiovascular Outcomes [IDACO] Investigators. Diagnostic thresholds for ambulatory blood pressure monitoring based on 10-year cardiovascular risk. Circulation 2007; 115: 2145-2152.
[6] Wolf-Maier, K.; Cooper, R.S. Banegas, J.R. et al. Hypertension prevalence and blood pressure levels in 6 European countries, Canada, and the United States. JAMA
[7] Wilkins, K.; Campbell, N.R; Joffres, M.R. et al. Blood pressure in Canadian adults. Health Rep 2010; 21: 37-46.
[8] Kannel, W.B. Blood Pressure as a cardiovascular risk factor: prevention and treatment. JAMA 1996; 275: 1571-6.
[9] Yusuf, S.; Hawken, S.; Ounpuu, S. et al. Effect of potentially modifiable risk factors associated with myocardial infarction in 52 countries [the INTER-HEART study]: case-control study. Lancet 2004; 364:937-952.
[10] Carretero, O.A and Oparil, S. Essential hypertension. Part I: definition and etiology. Circulation January 2000; 101(3): 329-335.
[11] AL-Turki Y. The prevalence of overweight and obesity amongst hypertensive and diabetic adult patients in primary health care. Saudi med J. 2000; 21(4): 340-343.
[13] Richard, N. Fogoros, MD. Hypertension Drugs. About. Com. Heart Health Center. 2011.
[14] Michael, L., Bishop and Larry's. Clinical Chemistry, 5th ed. Baltimore. U.S.A., 2005: 252.
[15] Crofton, P.M. Biochemistry of alkaline phosphatase isoenzymes. CRC. Crit. Rev. Clin. Lab. Sci. 1982; 16(3): 161-194.
[16] Trépanier, J.M; Seargeant, L.E. and Stinson, R.A. Affinity purification and some molecular properties of human liver alkaline phosphatase. Biochem. J. 1976; 155(3): 653-660.
[17] Varely H. and Gowenlock, A.H. Practical Clinical Biochemistry. 5th ed. London, 1984: 891, 899.
[18] Pospisilova, B.; Slizova, D.; Prochazkova, O.; Krs, O. and Bilek, P. Activity of Alkaline Phosphatase in The Major Salivary Glands of Mice at Various Ages of Postnatal Life, and During Pregnancy and Lactation. Acta Medica Hradec Kralove 1998; 41: 65-72.
[19] Henry J. Isoenzymes. 2nd ed. London, 1970.
[20] Kind P.R.N. and King E.J. Estimation of plasma phosphatase by determination of hydrolyzed phenol with amino-antipyrine. J. Clin. Path. 1954; 7: 322-326.
[22] Bashi, A.Y.D.; Jaber, R.K. and Al. Hamo, M. K. Measurement of certain liver function tests in hypertensive patients using Atenolol or Captopril. Journal of the Bahrain Medical Society 2010; 22(3): 98-102.
[23] El-Khawaga, O.Y.; El-Waseef, A.1; Ellazec, Y.O.; El-Naggar, M.M. and Abd Alla, M. Effect of Some Antihypertensive Drugs on Alkaline Phosphatase and DNA of Mice. International Journal of Genomics and Proteomics. 2013; 4[1]: 60-63.
[24] Liu, Y. Y.; Yao, W.M.; Wu, T.; Xu, B. L.; Chen, F. and Cui, L.Captopril improves osteopenia in ovariectomized rats and promotes bone formation in osteoblasts. J Bone Miner Metab. 2011; 29[2]: 149-158.
[25] Zubairi, M. B.; Ahmed, J. H; Al-Haroon, S. S. Effect of adrenergic blockers, carvedilol, prazosin, metoprolol and combination of prazosin and metoprolol on paracetamol-induced hepatotoxicity in rabbits. Indian J Pharmacol. 2014; 46(6): 644-648.
[26] Donald, V. and Judith G. V. Biochemistry. 4th ed., USA. 2011; 496.
[27] Harvey, R.A. and Ferrier, D.R. Lippincott 'S Illustrated reviews: Biochemistry. 6th ed. Wolters kluwer: LWW New York , 2014; pp54-57.