Study of synergistic Inhibitory effectiveness of mixed of Sweet bell pepper extract and virgin olive oil in α -amylase and α-glucosidase activity in serum of male rats infected with experimental diabetes
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Abstract
The present research designed for evaluate the inhibitory activities of red bell pepper alone ,red bell pepper and virgin olive oil together on alpha-amylase and alpha-glucosidase activities of adult male rats (Rattus norvegicus) infected with experimental Diabetes induced by alloxan, in present study used (40) male rats weight (250-300) gram, their age (14-16) weeks the experimental animals had been bought from faculty of veterinary/ university of mousl and had been performed in the animal house at Dept. of Biology of science college at Tikrit Univ. from October to December, The present study intend to screen novel alpha amylase and alpha glucosidase inhibitors from natural sources to management of diabetes mellitus type 2.
1- The results had been shown significant decrease at the level of (p<0.01) in serum glucose concentration in diabetics rats given mixed of red bell pepper and virgin olive oil in dose –dependent manner compare with diabetic group alone.
2- high significant increase (P<0.01) in insulin concentration in diabetics male rats given mixed red bell pepper and olive oil compared with diabetic group alone .
3- The present result had been shown high inhibitory percentage of mixed extract of red bell pepper and virgin olive oil together against alpha-amylase and alpha-glucosidase in diabetic rats more than red bell pepper alone in dose-dependent manner (2 ml, 4 ml, 6 ml, 8 ml/ kg. of body weight).
Conclusion: the mixture consist of red bell pepper extract and virgin olive oil show positive effect on inhibitory of amylase activity and alpha-glucosidase but effect on alpha- amylase more than on alpha glucosidase activity in male diabetic rats this may be considered this mixture as one of good management of hyperglycemia
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References
1-Sindhu, S. Nair, Vaibhavi, K.and Anshu M.(2013). In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts European Journal of Experimental Biology, 3(1):128-132
2-Shoback, edited by David G. Gardner, Dolores (2011). Greenspan's basic & clinical endocrinology (9th ed.). New York: McGraw-Hill Medical. pp. Chapter 17. ISBN 0-07-162243-8.
3- Ismail ,Bakaa H.(2008). Study of amylase enzyme and amylase creatinine clearance rate in non-insulin dependent diabetes meiilites .J. of Al-Anbar Univ., 1991-8941.
4- Green, J. H. (1985). Control system– Hormones. In: Basic clinical physiology. 3ed edition. Oxford medical publication. New York.Tornto. p 114-121.
5- Schmidt ,D.; Former ,H .;Junge ,B.;Muller M .; Wingender, W. and Trusheit E. (1999). a-Glucosidase inhibitor: New complex oligosaccharides of microbial origin. Natur wissenschaften 64, 535–536. 6- A. van de Laar, Floris ,Peter L. Lucassen,Reinier P. Akkermans,Eloy H. van de Lisdonk, M,Guy E. Rutten, and Chris van Weel, M.(2005). α-Glucosidase Inhibitors for Patients With Type 2 Diabetes ,j. Diabetes Care vol. 28 no. 1 154-163.
7-Mohammed, A. Adelaiye, A.B.; Bakari, A.G. and Mabrouk ,M.A.(2009) International Journal of Medicine and Medicina Sciences, , 1(12):530-535
8- Rodbard H.; Blonde L.;Braithwaite S.; Brett E.; Cobin R. and Handelsman Y.(2007). American Association of Clinical Endocrinologists medical guidelines for clinical practice for the management of diabetes mellitus. Endocr Pract. 13(Suppl 1):S1-68.
9-Bhat, M.; Zinjarde, S.S.; Bhargava, S.Y.; Kumar, A.R. and Joshi, B.N.(2011) Evi Based Complement Alternate Med,810-207.
10- Baldcci-Roslnd, E.; Silvirio, K.; Gorge, M. and Gonazaga, H. (2001). Effectof Isotrishone on tooth germ of plate development in mouse J embryos. Braz. Dent., 12(2): 115-119.
11- Kyung, K. and Felming, H.(1994). Antibacterial activity of cabage juice against lactic acid bacteria . J. Food Sci., 59(1) : 125-129 .
12- Young .D.S.effect of drugs on clinical laboratory tests,4th ed.(1995),p:3-43 to 3-46.
13-Kim, Y. et al. (2003). Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition 21(6): 756 – 761.
14-Young- K, Emmanouil A and Kalidas ,S.(2007) Evaluation of pepper (CAPSICUM ANNUUM) for management of Diabetes and Hypertenstion, J. of Food Biochem., 31 :370–385.
15- Ademosun A., Oboh, G.*, Adewuni T., Akinyemi A. and T. A. Olasehinde. (2013).
Antioxidative properties and inhibition of key enzymes linked to type-2 diabetes by snake tomato (Tricosanthes cucumerina) and two tomato (Lycopersicon esculentum) varieties African J. of pharmacy and pharmacology,7:33,pp.2358-2365.
16- Tadera, K.; Minami, Y.; Takamatsu, K. and Matsuoka, T. (2006)Inhibition of alpha-glucosidase and alpha-amylase by flavonoids. J. Nutr., Sci. Vitaminol, (Tokyo).52(2):149-53.
17- Erbay, Z. and Icier ,F.(2010) The importance and potential uses of Olive leaves. Food Rev Int . 26(4): 319 -34.
18- Tuck, K.L. and Hayball P.J. (2002)Major phenolic compounds in olive oil: metabolism and health effects. J. Nutr, Biochem.13(11):636-44.
19- Van de Laar F.A.(2008) Alpha-glucosidase inhibitors in the early treatment of type 2 diabetes. Vasc Health Risk Manag.4(6):1189-95.
20 -Van de Laar F.A., Lucassen, P.L.and Akkermans R.P.(2005) Alpha-glucosidase inhibitors for type 2 diabetes mellitus. Cochrane Database Syst Rev. 21-Minh A.,Jin W., Jingyi T., Yan Z. and Ken Ng(2013).Evaluation of α-glucosidase inhibition potential of some flavonoids from Epimedium brevicornum, 492–498. 22- Al Jamal Abdul Rahim and Abdallah Ibrahim .(2011).Effects of olive oil on lipid profiles and blood glucose in type2 diabetic patients . Int J Diabetes & Metab, 19:19-22.
23- Paiva-Martins, F.; Fernandes, J.; Rocha,S.; Nascimento, H.; Vitorino, R.; Amado,F.; Borges, F.; Belo,L. and Santos-Silva, A.(2009). Effects of olive oil polyphenols on erythrocyte oxidative damage. Mol Nutr.Food Res; 53: 609-616.
24- Jung,U.; Lee,M.; Jeong,K.and Choi,M.(2006). The hypo- glycemic effects of hesperidin and naringin are paetly mediated by hepatic glucose-regulating enzymes in C57 BL/KSJ-db Mice. J. Nutr; 134(10): 2499-2503.
25-Navarro, J.; Floresand, P. and Garrido ,C. (2006). Vicente Martinez. Changes in the contents of antioxidant compounds in pepper fruits at different ripening stages, as affected by salinity Food Chemistry 96: 66–73.