Spectrophotometric method for Determination of oxymetazoline, HCl in a Pharmaceutical formulation using 2,4 - dinitrophenylhydrazine
Article Sidebar
Main Article Content
Abstract
Rapid, sensitive and simple spectrophotometric method for the determination of oxymetazoline hydrochloride (OXMZ) is developed. The method is based on an oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 11.2) in the presence of potassium periodate to produce a green colour, soluble in water , stable product and absorbs at 607 nm. Beer's law was in the linear range 2.5-30 μg/ml of OXMZ, the molar absorptivity , Sandellʼs sensitivity index and detection limit were 7242 liter. mol-1.cm-1, 0.0409 μg.cm-2 and 0.1877 μg/ml respectively. The RSD value was 1.24- 1.39% depending on the concentration. This method was applied successfully to the determination of oxymetazoline in pharmaceutical preparation nazordin drops with recovery of not less than 99.87 % .
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
1- British Pharmacopeia, 6th Ed., by system simulation
ltd., the stationary office, London, (2009). in CD-ROM"
2- G.R. Spratto and A.L. Woods ;Nurseʼs Drug Handbook; Delmar, Cengage learning, (2009).
3- S. B. A. Yosif ;Determination of amitriptyline hydrochloride and oxymetazoline hydrochloride in pharmaceutical preparations by membrane selective
electrodes and by spectrophotometric method; " M.Sc. Thesis", University of Tikrit, College of
Education, 71- 84,(2010).
4- S.A. Zakaria; Spectrophotometric determination of oxymetazoline hydrochloride via oxidative coupling reaction with 4-aminoantipyrine in the presence of potassium periodate; Raf. J. Sci., 22(4) , 97-108, (2011).
5- T. N. Al-Sabha and B. A. Rasheed; Spectrophotometric determination of oxymetazoline hydrochloride based on the oxidation reactions; Jordan J. of Chem., 6 (4), 403-411,( 2011).
6- N. S. Othman and S. A. Fathe; Indirect spectrophotometric determination of oxymetazoline hydrochloride; Raf. J. Sci.,24(1), 84-95, (2013).
7- O. Abdel-Aziz, A.M. El-Kosasy, N. Magdy and N.M. El Zahar; Novel spectroscopic methods for determination of Cromolyn sodium and oxymetazoline hydrochloride in binary mixture;
Spectrochim. Acta. A Mol. Biomol. Spectrosc., 131, 59–66, (2014).
8-T. J. Hoffmann, R. D. Thompson and J. R. Seifert; Determination of the nasal decongestant, oxymetazoline hydrochloride, in pharmaceutical
formulations by HPLC; Drug Development and
Industrial Pharmacy 15(5), 743-757,(1989).
9- R.T. Sane, L.S. Joshi, K.D. Ladage, R.M. Kothurkar
and V.R. Bhate; High performance liquid chromatographic determination of oxymetazoline
hydrochloride from nasal drops; Indian J. Pharm. Sci., 52(1),38-39,(1990).
10- D. D. Orsi, L. Gagliardi, G. Cavazzutti, M. G. Mediati and D. Tonelli; Simultaneous determination of ephedrine and 2- imidazolines in pharmaceutical formulations by reversed-phase HPLC; Journal of Liquid Chromatography, 18( 16), 3233- 3242,(1995).
11- F.J. Hayes, T.R. Baker, R.L. Dobson and M.S. Tsueda; Rapid liquid chromatographic-mass spectrometric assay for oxymetazoline in whole rat
blood; J. chromatogr. A, 692(1- 2), 73-81,(1995).
12- B. Stanisz and W. Nowinski; Determination of oxymetazoline hydrochloride and decomposition products by high performance liquid chromatography; Acta Poliniae Pharmaceutica., 57(6),399-401,(2000).
13- S. Sudsakorn, L. Kaplan and D.A. Williams ;Simultaneous determination of triamcinolone acetonide and oxymetazoline hydrochloride in nasal spray formulations by HPLC; J. Pharm.Biomed. Anal. ,40(5),1273-1280,(2006).
14- Y.Y. hong; Content determination of oxymetazoline hydrochloride spray by HPLC; Strait Pharmaceutical Journal, 9, 31-33, ( 2009). 15- K. A. Shaikh and A.T. Patil; Stability-indicating HPLC method for the determination of mometasone furoate, oxymetazoline, phenyl ethanol and
benzalkonium chloride in nasal spray solution; Journal of Trace Analysis in Food and Drugs,1, 14-21,
(2013).
16- C. Naijiang, Z. Hui and Z. Jun; Study on the quality standard of Lidocaine and Oxymetazoline Solution; Northwest Pharmaceutical Journal,6, 593-
595,(2013).
17- Y.M. Issa and S.I.M. Zayed; Construction and analytical applications of plastic membrane electrode for oxymetazoline hydrochloride; Anal. Sci., 20(2), 297-300,( 2004).
18- H. Abd Al-Razaq; Construction of oxymetazoline hydrochloride selective electrodes and determination of oxymetazoline in pharmaceutical drugs; "M.Sc. Thesis", university Al-nahrain, college of science, (2007).
19- N. M. Mahmoud; New sensors for the determination of the pharmaceutical compound oxymetazoline hydrochloride; "M.Sc.Thesis",
university of beni-suef, college of science, 88, (2011).
20- M.A. Ali; Preparation, comparison and characterization of dual drug (promethazine& oxymetazoline) selective electrode; J. of Al-Nahrain University ,15 (3),80-87, (2012).
21-A. M. Garcia-Campana, J. M. B. Sendra, M. P. B. Vargas, W. R.G. Baeyens and X. Zhang; Flow injection analysis of oxymetazoline hydrochloride with inhibited chemiluminescent detection; Analytica. Chimica. Acta., 516( 1-2,), 245–249, (2004).
22- N.Wang, Y. Shao, Y. Tang, H. Yin and X. Wu; Flow-injection chemiluminescence method for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride; Luminescence, 24( 3), 178 - 182, ( 2009).
23- D. D. Perrin and B. Dempsey ;Buffers for pH and Metal Ion Control; Chapman and Hall , Ltd. , London , 1974 , 130 - 134.