Assessment of Inhibin-B hormone level and its relationship with some gonadal hormones in males having idiopathic infertility
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Abstract
The aim of the current study to determine levels of Inhibin-B hormone and its relationship with some gonadal hormones, including follicle-stimulating hormone (FSH), luteinizing hormone (LH) prolactin, and testosterone hormones in some males with idiopathic infertility in Shirqat city.
Blood and seminal fluid samples from (60) idiopathic male infertile and (30) healthful individuals as (control group) aged (18 to 60 years) were collected from private clinics. Enzyme-linked immunosorbent assay (ELISA) was used to estimate serum Inhibin B, FSH, LH, prolactin, and testosterone hormones.
According to types of sperm count, patients have divided onto two groups 49 persons as azoospermia group (zero /ml) and 11 persons as oligospermia group less than 20 million/ml. Inhibin B and testosterone levels were significantly decreased (P≤0.01) in the infertile men compared with the control group. While the FSH level, LH, and prolactin were significantly higher (P≤ 0.01) in the infertile men than in the control group, the results of Inhibin B were non-significant.
In contrast, levels of FSH, LH, and prolactin were higher significant (P≤ 0.01), except testosterone level was significant differences (P≤ 0.05) in the age groups <20-30 year, 31–40 year, and ≥ 40 years. There were significant differences (P≤ 0.01) in levels of FSH and prolactin, but the levels of Inhibin B and LH were non-significant in the infertility period ≤ 10 year, 11 – 20 years, ≥ 20 years. Smoker infertile men have low levels (P≤ 0.01) in the prolactin while had high levels in the Inhibin B and LH hormones compared with control. Patients with a family history showed a significant difference (P≤0.05) in the levels of Inhibin B, testosterone, LH, and prolactin.
In conclusion, this study revealed a significant decrease in the Inhibin-B levels of the Azoospermic and oligospermia infertile men. The negative correlation between inhibin B and testosterone and weak correlation with other gonadal hormones. Therefore, Inhibin-B has an important role in diagnosing idiopathic male infertility, and it's one of the important markers in the diagnosis of normal spermatogenesis.
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References
[1] Salsabili, N. et al. (2011) ‘Gonadotropin and Testosterone hormone’s serum levels and partial deletions in the AZFc region in Iranian oligozoospermia infertile males’, Health. Scientific Research Publishing, 3(9), pp. 566–570.
[2] Nygren, K. G. and Andersen, A. N. (2001) ‘Assisted reproductive technology in Europe, 1997. Results generated from European registers by ESHRE. European IVF-Monitoring Programme (EIM), for the European Society of Human Reproduction and Embryology (ESHRE).’, Human Reproduction (Oxford, England), 16(2), pp. 384–391.
[3] Poongothai, J., Gopenath, T. S. and Manonayaki, S. (2009) ‘Genetics of human male infertility’, Singapore Med J, 50(4), pp. 336–347.
[4] Ferlin, A. et al. (2007) ‘Male infertility: role of genetic background’, Reproductive biomedicine online. Elsevier, 14(6), pp. 734–745.
[5] O’Brien, K. L., Varghese, A. C. and Agarwal, A. (2010) ‘The genetic causes of male factor infertility: a review’, Fertility and sterility. Elsevier, 93(1), pp. 1–12.
[6] Jungwirth, A. et al. (2015) ‘Guidelines on male infertility’, Eur Urol, 62, pp. 324–332.
[7] Guyton, A. C. and Hall, J. E. (2006) ‘Medical physiology’, Gökhan N, Çavuşoğlu H (Çeviren), 3.
[8] Pierik, F. et al. (1998) ‘Serum inhibin B as a marker of spermatogenesis’, Journal of clinical endocrinology and metabolism.
[9] Kumanov, P. et al. (2006) ‘Inhibin B is a better marker of spermatogenesis than other hormones in the evaluation of male factor infertility’, Fertility and sterility. Elsevier, 86(2), pp. 332–338.
[10] Illingworth, P. J. et al. (1996) ‘Inhibin-B: a likely candidate for the physiologically important form of inhibin in men.’, The Journal of Clinical Endocrinology & Metabolism. Oxford University Press, 81(4), pp. 1321–1325.
[11] Yalti, S., Gürbüz, B. and Fiçicioğlu, C. (2002) ‘Serum levels of inhibin B in men and their relationship with gonadal hormones, testicular volume, testicular biopsy results and sperm parameters’, Journal of Obstetrics and Gynaecology. Taylor & Francis, 22(6), pp. 649–654.
men and their relationship with gonadal hormones, testicular volume, testicular biopsy results and sperm parameters. J Obstet Gynaecol (Lahore). 2002;22(6):649–54.
[12] Shaya, R. E. and Al-Dabbagh, N. Y. (2013) ‘Detection of Antisperm Antibodies in Relation to Inhibin B in Infertile Men’, Rafidain journal of science. Mosul University, 24(3E), pp. 1–16.
[13] Al-Khateeb, S., Hussein, S. M. and Dahy, A. A. A. I. (2016) ‘Evaluation of inhibin-B hormone, FSH, and Testosterone in serum of infertile men’, Journal of the Faculty of Medicine. Baghdad University, 58(2), pp. 180–182.
[14] Al-Obaidy, E. N. J. and Al-Samarrai, A.-M. H. (2017) ‘Relationship of Inhibin-B with Gonadal Hormones Levels in Seminal Plasma of Infertile Patients in Diyala Governorate’, Iraqi Journal of Embryos and Infertility Researches. Al-Nahrain University, 7(1), pp. 37–43.
[15] Omear, H. A. (2019) ‘Effect of Inhibin-B hormone and its relationship with a number of sex hormones in men with Azoospermia’, Tikrit Journal of Pure Science, 24(3), pp. 11–16.
[16] Zalata, A. A. et al. (2008) ‘Follicle‐stimulating hormone receptor polymorphism and seminal anti‐Müllerian hormone in fertile and infertile men’, Andrologia. Wiley Online Library, 40(6), pp. 392–397.
[17] Safarinejad, M. R., Shafiei, N. and Safarinejad, S. (2011) ‘Evaluating the role of the FSH receptor gene Thr307‐Ala and Asn680‐Ser polymorphisms in male infertility and their association with semen quality and reproductive hormones’, Bju International. Wiley Online Library, 108(2b), pp. E117–E125.
[18] Grigorova, M. et al. (2013) ‘Study in 1790 Baltic men: FSHR Asn680Ser polymorphism affects total testes volume’, Andrology. Wiley Online Library, 1(2), pp. 293–300.
[19] Al-Nahi, A. S. (2015) ‘Evaluation of FSH, LH, Testosterone, Prolactine, TSH and T4 hormones levels in different subgroups of infertile males.’, Al-Kufa University Journal for Biology. University of Kufa, 7(3), pp. 47–54.
[20] AL-Shammaa, N. M. J. (2015) ‘Correlation of Gonadel Hormones and Insulin Resistance with Seminal Fluid in Infertile Males Afflicted with Diabetes Mellitus’, Al-Mustansiriyah Journal of Science. Al-Mustansyriah University, 26(1), pp. 24–31.
[21] Crook, M. (2013) Clinical biochemistry and metabolic medicine. CRC Press.
[22] Matzkin, H. et al. (1990) ‘Serum bioactive and immunoreactive follicle-stimulating hormone in Oligozoospermic and azoospermic men: application of a modified granulosa cell bioassay’, Fertility and sterility. Elsevier, 53(4), pp. 709–714.
[23] Sherwood, L. (2004) ‘The Reproductive System: Male Reproductive Physiology. In’, Human Physiology. Julet, M, pp. 749–770.
[24] Meeker, J. D., Godfrey‐Bailey, L. and Hauser, R. (2007) ‘Relationships between serum hormone levels and semen quality among men from an infertility clinic’, Journal of andrology. Wiley Online Library, 28(3), pp. 397–406.
[25] Bohring, C. and Krause, W. (1999) ‘Serum levels of inhibin B in men with different causes of spermatogenic failure’, Andrologia. Wiley Online Library, 31(3), pp. 137–141.