Prevalence of Vaginal Candidiasis among women and Diagnosis of Candida species from vaginal infection in Kirkuk city

Main Article Content

Bari lateef mohammed
Kalil I. Bander
Thekra A. Hamad

Abstract

Vaginal candidiasis is a common complaint among women of different age groups in any society whether or not they are sexually active. Although it is both treatable and mild, when left untreated, The study was set to detect Candida organisms in women vaginal. A prospective study of women vaginal swabs collected from Gynecological Clinic in Azadi teaching Hospital and some privet clinical in Kirkuk city and a total of two hundred   (200) women swab were  analysed for microscopy and culture, from May 2014 to April 2015. Data on epidemiologic indices were collected from the patients, using structured interviewer-administered questionnaires. fifty- seven positive cultures were obtained, constituting 28.5% (n = 57) in a total of 200 women vaginal samples. A total of 28.5% (n= 57) isolates were detected which comprises four different Candida species, namely Candida albicans, C. glabrata, C. krusei and C. kefyre respectively as well as isolated Cryptococcus lorynti with frequency of  occurrence of 23%, 2%, 1.5%, o.5% and 1.5% respectively. The isolates were related to age, the age range of 26-35 years had the highest incidence (35%), followed by age range of 36-45 years (29.5%) and 56-88 years had the least (2%) percentage of occurrence. The isolates were also related to used contraceptives, copper-T contraceptives and combined oral contraceptives 41.5% was the most common methods used by recurrent vaginitis patients while the male condom contraceptives users had lower cure (26.5%). The distribution of vaginal candidiasis according  antibiotic using was highest in women take antibiotic with 64.4% of the total 200 cases, while the women don’t take antibiotic was recorded lowest occurrence (35.5%). The result shows that Candida species has assumed the role of the most common cause of vaginitis, with Candida albicans as the most prevalent species. vaginal candidiasis was common in the young adults o age range of 26-35 years, contraceptive , broad spectrum antibiotic users ,other diseases and operation .We therefore recommend prevention, early diagnosis and prompt treatment of vaginal candidiasis especially among the risk groups in order to avert its complications.

Article Details

How to Cite
Bari lateef mohammed, Kalil I. Bander, & Thekra A. Hamad. (2023). Prevalence of Vaginal Candidiasis among women and Diagnosis of Candida species from vaginal infection in Kirkuk city. Tikrit Journal of Pure Science, 20(4), 5–15. https://doi.org/10.25130/tjps.v20i4.1206
Section
Articles

References

1. Sobel, J. D., and W. Chaim. (1996). Vaginal microbiology of women with acute recurrent vulvovaginal candidiasis. J. Clin. Microbiol. 34: 2497 –2499.

2. Manolakaki, D.,Velmahos, G., Kourkoumpetis, T., Chang, Y., Alam, H. B., De Moya, M. M., & Mylonakis, E. (2010). Candida infection and colonization among trauma patients. Virulence, 1(5), 367-375.

3. Kourkoumpetis, Themistoklis K. (2011) "The effect of cumulative length of hospital stay on the antifungal resistance of Candida strains isolated from critically ill surgical patients." Mycopathologia 171.2: 85-91.Odds FC. (1989) Candida and Candidosis 2d ed. London: Baillie`re-Tindall, pp 68–92.

4. Sobel, J. D. (2006). The emergence of non-albicans Candida species as causes of invasive candidiasis and candidemia. Curr. Infect. Dis. Rep. 8:427–433.

5. Walsh, TJ. Hiemenz JW. , Anaissie EJ . (1996) Recent progress and current problems in treatment of invasive fungal infections in neutropenic patients. Infect Dis Clin North Am 10:365–400,.

6. Sobel, J. D., S. Faro, R. W. Force, B. Foxman, W. J. Ledger, P. R. Nyirjesy, B. D. Reed, and P. R. Summers. (1998). Vulvovaginal candidiasis: epidemiologic, diagnostic, and therapeutic considerations. Am. J. Obstet. Gynecol. 178:203–211.

7. Odds, F. C., and R. Bernaerts. 1994. CHROM agar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J. Clin. Microbiol. 32:1923–1929.

8. Pfaller, M. A., A. Houston, and S. Coffmann. 1996. Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. J. Clin. Microbiol. 34:58–61.

9. Baker, F.( 1967) .“Handbook of Bacteriological Technique,” 2nd Edition, Butterworth Co. Ltd., London, , pp. 415- 421.

10. Tasna T, Kitch L, Michael R. (1996). Ability of RapID Yeast Plus System To Identify 304 Clinically Significant Yeasts within 5 Hours,. J Clin Microbiol. May;34: 1069–1071 .

11. AL-Hachami SH.N. (2004) Isolation and Diagnosis candida albicans from vaginal and Study of Virulence Factors and sensitivity to Antifungal Agents .A thesis ,College of Education (Ibn AL-Haitham) ,University of Baghdad .

12. Mikolajczyk K, Zimmer M, Tomialowicz M, Fuchs T. (2006) Mikologia Lekarska;13 (3): 175-179.

13. Limia OF, Lantero DMI. (2004) . Medscape Geral Medicine;6(4): 50.

14. Esmaeilzadeh S, Omran SM, Rahmani Z. (2009) Intl J Fertility Sterility;3 (2): 74-77.

15. Jombo GTA, Akpera MT, Hemba SH, Eyong KI. (2011) African J Cli Exp Microbiol;12(1): 32- 37.

16. Shivan D, Saldanha DRM. J. (2011) Clin Diagnostic Res;5(6): 1177-1181.

17. Rad MM, Zafarghand ASh, Zabihi MA, Tavallaee M, Mirdamadi Y. (2012) Inf Dis Obs Gynecol;1-5.

18. Isibor JO, Samuel SO, Nwaham CI, Amanre IN, Igbinovia O, Akhile AO. (2011) African J Microbiol Res;5(20): 3126-3130.

19. Kalkanci A, GüZel AB, Israa Ibrahim Jabban Khalil I I J, Aydin M, Ilkit M & Kuştimur S. (2012) Yeast vaginitis during pregnancy: susceptibility testing of 13 antifungal drugs and boric acid and the detection of four virulence factors Medical Mycology August; 50(6): 585-593.

20. Wise MG, Healy M, Reece K, Smith R, Walton D, Dutch W, Renwick A, Huong J, Young S, Tarrand J, Kontoyiannis D. J. (2007) Med Microbiol;56 (6): 778-787.

21. Trofa D, Gácser A, Nosanchuk JD. (2008) Clin Microbiol Rev;21 (4):606–625.

22. Kenneth OB. (2003). STDs/HIV/AIDS -Challenge of next millennium. Nigeria. J. Genitourinary Med., 18: 18-30.

23. Willacy H, Jackson C. (2011). Vaginal and Vulval Candidiasis. Online at Patient. co.uk/ doctor/ Vaginal- and- Vulval-Candidiasis.htm.

24. Thulkar, J.; Kriplani, A.; Agarwal, N. and Vishnubhatla, S. (2010) . Aetiology & risk factors of recurrent vaginitis & its association with various contraceptive methods. Indian . J. Med Res 131, pp 83-87.

25. Diddle, A.W.; William, H.; Perry, J.and Kenneth, A. (1969) Oral contraceptive Medication and Vulvovaginal Candidiasis ,Vol.34 ,No. 3.

26. Diddle A.W , William H.,Williamson J. and Kenneth A.(1967).Oral Contraceptive Medication and Vulvovaginal Candidiasis. Editorial . Contraceptive pill .New Eng J Med, 276:239

27. John EE. (2000). Mycosis. In: Mandell G L, Bennett J E, Dollin R ,.(editors) Textbook of Principles and Practice of Infectious diseases. 5th Ed. New York: Churchill Livingstone, pp. 2291.

28. Sobel, J. D. (2007). Vulvovaginal candidosis. Lancet 369:1961–1971.

29. Ronnqvist, P. D., Forsgren-Brusk, U. B. and Grahn-Hakansson E. E.( 2006). Lactobacilli in the female genital tract in relation to other genital microbes and vaginal pH. Acta Obstet. Gynecol. Scand. 85:726–735.

30. Vitali, B., C. Pugliese, E. Biagi, M. Candela, S. Turroni, G. Bellen, G. G. G. Donders, and P. Brigidi. (2007). Dynamics of vaginal bacterial communities in women developing bacterial vaginosis, candidiasis, or no infection, analyzed by PCR-denaturing gradient gel electrophoresis and real-time PCR. Appl. Environ. Microbiol. 73:5731–5741.

31. Federal Ministry of Health (FMOH) (2005). A manual of technical report on the National HIV/Syphilis sentinel survey among pregnant women attending Antenatal clinics in Nigeria. Abuja. Nigeria. pp. 1-11.

32. Abebe EA, Olumide M., Oke O. (2001).A manual for Health workers on Syndromic Management of STI. National AIDS and STD control program; Federal Ministry of Health Abuja. pp. 3-7.

33. Saigal S, Bhargava A, Mehra SK, Dakwala F. (2011) Contemporary Clinical Dentistry; 2(3): 188–193.

34. Giraldo PC, A raújo ED, Junior JE, Amaral RLG, Passos MRL, Gonçalves AK. (2012) . Inf Dis Obs Gynecol;1-4.

35. Williams OT, Joy OA, Ugonnal NP, Chukwuemeka IJ, Kester UE. (2012) International Journal of Pharmaceutical Research and Development;4(3):323-331.