Determine the Ability of Bacterial Isolates from Urinary Tract Infections on L-Asparginase Production
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Abstract
This study was conducted to isolate and identify the pathogenic bacterial species from urinary tract of patients that reviewers to Tikrit hospital education from tow gender and different age periods. It was also the determination of bacterial species isolated in their ability to production of the L-asparginase enzyme and assay the environmental conditions and the production media ingredients for high concentration of enzyme production. Results showed that 142 samples was positive in microbial isolation (91.64%) from the total study samples that were at 150 samples, and high isolation percentage was from female at 58% and from aged period between 15 to 75 years for two gender. It also found that the species of bacterial E.coli was appear as highest in isolation percentage followed by Citrobacter diversus, Enterobacter aerogenes and Klebsiella pneumonia which was at 54.2, 21.1, 17.6 and 7.04% respectively. The assay of bacterial isolates to L-asparginase produce illustrate that the E.coli species appear as the most production and the isolate no. 3 was the top one at 70.4 IU/ml of media. When the assay of environmental conditions and ingredients combination for media to access for higher production from enzyme, shows that the optimal conditions for production of E.coli-Iso.3 was in development at 35 º c, pH 7.0 for 24 hours with agitation at 150 rpm in the media consists of maltose, L-asparginase, NaCl and KH2PO4 at 10, 100, and 5.0 and 0.75 g/l respectively.
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References
1) Abu-shagra, Q. 2000. Occurance and antibiote sensitivity of Enterobacteriaceae isolated from group of Jordanian patients with community acquired urinary Tract Infection. Cytobios.
2) Alfred , E.B. 2005. Microbiological application in the laboratory manual in general microbiology .(9th ) ed. MC Grow Hill companies.
3) Aung, H.P., M. Bocola, S. Schleper and K.H. Rohm, 2000. Dynamics of a mobile loop at the active site of Escherichia coli asparaginase. Biochimica Biophysica Acta (BBA)-Protein Struct. Mol. Enzymol., 1481: 349-359.
4) Atalla, M.M ; M. M. Farag ; R. H. Eman ; M. S. Abd-El-lataif and E. A. Nehad , 2009 . Malaysian J. of microbiology , vol. 5(1) 2009, p.45-50.
5) Boyse, E.A., L.J. Old, H.A. Campbell and L.T. Mashburn, 1967. Suppression of murine leukemias by L-asparaginase. Incidence of sensitivity among leukemias of various types: Comparative inhibitory activities of guinea pig serum l-asparaginase and Escherichia coli L-asparaginase. J. Exp. Med., 125: 17-31.
6) Cohn, E.B. and Schaeffer, A.J. 1998. Urinary tract infection in adults. Northwestern Medical School, J. of Urol.77:122-130.
7) Collee, J.G.; Franser, A.G.; Marmion, B.P. and Sinmons, A. 1996. "Mackie and MacCartney practical medical microbiology". 4th. (Ed.). Churchill Livingstone, London.
8) Delzell, J.E. and Lefevre, M.L. 2000. Urinary tract infection during pregnancy. Am. Academy of Family Physicians, 35(3):40-66
9) Derst C, Wehner A, Specht V, Rohm KH. 1994. States and functions of tyrosine residues in Escherichia coli asparaginase. Eur J Biochem. 224:533-540.
10) Dhevagi, P. and E. Poorani, 2006. Isolation and characterization of L-Asparaginase from marine actinomycetes. Ind. J. Biotech., 5: 514-520.
11) Duncan.D .B. 1955.Multiple range and multiple Ftest,Biometric,11,1-42.
12) Eden, O.B., M.P. Shaw, J.S. Lilleyman and S. Richards, 1990. Non-randomised study comparing toxicity of Escherichia coli and Erwinia asparaginase in children with leukaemia. Med. Pediatric Oncol., 18: 497-502.
13) El-Bessoumy, A.A., M. Sarhan and J. Mansour, 2004. Production, isolation and purification of L-asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation. J. Biochem. Mol. Biol., 37: 387-393.
14) Eykan, S.J. 2001. Value disease: Endocarditis: basics. Heart. 86 – 480.
15) Fowler, B. A. ; Nordberg , G. F. Nordber, M. and Friberg g, L. 2007 . Hand book on the Toxicology of metals . Academic Press, Inc.
ISSN:1813 – 1662 2015 ) 5( مجلة تكريت للعلوم الصرفة، 20
10
16) Gillespie, S.A., and Hawkey, P.M. 2006. Principles and practice of clinical bacteriology, 2nd ed., John Wiley & Sons Ltd.
17) Givry, S. and F. Duchiron .2008 .Optimization of culture medium and growth condition for production L-arabinose isomerase and D-xylose by lactobacillus bifermentans. Microbiology. 77 : 281-287
18) Hummers-Pradier. E. 2004. Ohse AM, Koch M, Heizmann WR, Imada A, Igarasi S, Nakahama K, Isono M (1973). Asparaginase and glutaminase activities of micro-organisms. J Gen Microbiol: 76, 85-99.
19) Kumar, K. and N. Verma, 2012. The various sources and application of L-asparaginase. Asian J. Biochem. Pharma. Res., 3: 197-205.
20) Krasotkina, J., A.A. Borisova, Y.V. Gervaziev and N.N. Sokolov, 2004. One-step purification and kinetic properties of the recombinant l-asparaginase from Erwinia carotovora. Biotechnol. Appl. Biochem., 39: 215-221.
21) Mesas, J.M., J.A. Gil and J.F. Martin, 1990. Characterization and partial purification of l-asparaginase from Corynebacterium glutamicum. J. Gen. Microbiol., 136: 515-519.
22) Narta, U.K., S.S. Kanwar and W. Azmi, 2007. Pharmacological and clinical evaluation of L-asparaginase in the treatment of leukemia. Crit. Rev. Oncol. Hematol., 61: 208-221.
23) Nester, E.W.; Anderson, D.G.; Robert, C.E.; Pearsall, N.N. and Nestor, M.T. 2001. Microbiology a human perspective.3 rd ed.Mc Graw-Hill Higher Education .,291-295.
24) Orenstein, R. and Wong, E. 1999. Urinary tract infection in adults. American Academy of Family Physicians, 34(3): 70-79 .
25) Patro, K.K.R., S. Satpathy and N. Gupta, 2011. Evaluation of some fungi for L-asparaginase production. Indian J. Fundam. Applied Life Sci., 1: 219-221.
26) Pourhossein, M. and H. Korbekandi, 2014. Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli. Adv. Biomed. Res., Vol. 3. :10-41.
27) Prakasham, R.S., Ch.S. Rao, R.S. Rao, G.S. Lakshmi and P.N. Sarma, 2007. L-asparaginase
production by isolated Staphylococcus sp. -6A: Design of experiment considering interaction effect for process parameter optimization. J. Applied Microbiol., 102: 1382-1391.
28) Prescott L.M., Harley J.P., Klein D.A. Procaryotic Cell Structure and Function. In: Introduction to Microbiology. 7th ed. New York, USA, The McGraw-Hill, 2010, pp37-72
29) Raha, S.K., S.K. Roy, S.K. Dey and S.L. Chakrabarty, 1990. Purification and properties of an L-asparaginase from Cylindrocarpon obtusisporum MB-10. Biochem. Int., 21: 987-1000.
30) Raz, R. (2001). Post menopausal woman with recurrent UTI. Int. J. Antibiotic Agents. 17: 269 – 271.
31) Rehman, K;. Rajoka, M. I.; Tabish, T. and Zia, M. A. , 2006. J. of microbiology & biotechnology, 22: 288-291.
32) Reynolds, D.R. and J.W. Taylor, 1993. The Fungal Holomorph: A Consideration of Mitotic Meiotic and Pleomorphic Speciation. CAB International, Wallingford, UK.
33) SAS users (2004).Guid personal computer (ver.7) inst. Inc. Cary. Nc. USA.
34) Story, M.D., D.W. Voehring, L.C. Stephens and R.E. Mern, 1993. L-asparaginase kills lymphoma cells by apoptosis. Cancer Chemother. Pharmacol., 32: 129-133.
35) Vandepitte, J; Eng back, K.; Piot P Heuck, C.C. 2003. and Basic Laboratory procedures in clinical bacteriology, World Health Organization, Geneva.
36) Vandepitte, J; Eng back,K.; Piot P. and Heuck, C.C. 1991. Basic Laboratory procedures in clinical bacteriology, World Health Organization, Geneva.
37) Verma, N., K. Kumar, G. Kaur and S. Anand, 2007. L-asparaginase: A promising chemotherapeutic agent. Crit. Rev. Biotechnol., 27: 45-62.
38) Winn, J. W. ; Allen, S. ; Janda, W. ; Koneman, E. ; Procop, Schreckenberger, P. and Woods, G. 2006. “Konemans Color Atlas and Textbook of Diagnostic Microbiology” 6th ed., Lippincott Williams and Wilkins, U.S.A
39) Yoon ,S.H , P.Kim and D. Koh .2003. Properties of L- arabinose isomerase from Escherichia coil as biocatalyst for tagatose production World J .Microbiol. , 19:47-51 .