The Impact of G6PD Deficiency on Liver Function in Children Under 15
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Abstract
Glucose-6-phosphate dehydrogenase (G6PD) is involved in the first phase of the pentose phosphate pathway (PPP), entailing the reduction of nicotinamide adenine dinucleotide phosphate (NADP+) and the oxidation of glucose-6-phosphate to 6-phosphogluconolactone. A deficiency in the G6PD activity can result in numerous illnesses, including liver diseases. The first three enzymes in PPP are responsible for the oxidative phase. They make NADPH, which is needed to deal with oxidative stress, especially in people who do not have enough G6PD. The study included (90) samples from people with hemolytic anemia (Favzim) and control to determine the levels of G6PD, albumin, total protein, liver function, alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and bilirubin. The results showed that there was a significant decrease (P<0.05) in the serum levels of G6PD in the patients group when compared with the control group. Also, there was a significant increase (P<0.05) in the serum levels of albumin, total protein, ALP, ALT, AST and total bilirubin in patients with the G6PD deficiency when compared with the control group. In addition, this study revealed a strong positive correlation between some biochemical parameters studied, such as: Total protein with TSB (r2 = 0.897), TSB with ALT (r2 = 0.893), AST with ALT (r2 = 0.623) and total protein with ALT (r2 = 0.804).
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References
[1] Li, W., Liu, X., Cheng, X., Zhang, W., Gong, C., Gao, C., ... & Tao, H. (2022). Effect of Malt-PEG-Abz@ RSL3 micelles on HepG2 cells based on NADPH depletion and GPX4 inhibition in ferroptosis. Journal of Drug Targeting, 30(2), 208-218.
[2] N. Maheshwari, N. Qasim, R. Anjum, and R. Mahmood. (2020). “Ecotoxicology and Environmental Safety Fluoride enhances generation of reactive oxygen and nitrogen species , oxidizes hemoglobin , lowers antioxidant power and inhibits transmembrane electron transport in isolated human red blood cells,” Ecotoxicol. Environ. Saf., vol. 208, p. 111611, 2021, doi: 10.1016/j.ecoenv.111611.
[3] Z. Liu et al. (2020). “Chinese newborn screening for the incidence of G6PD deficiency and variant of G6PD gene from 2013 to 2017,” Hum. Mutat., vol. 41, no. 1, pp. 212–221, doi: 10.1002/humu.23911.
bbbb Conclusions: Figure (1): Correlation between total protein and TSB Figure (2): Correlation between TSB and ALT Figure (3): Correlation between AST and ALT Figure (4): Correlation between total protein and ALT
[4] F. Rajas, A. Gautier-Stein, and G. Mithieux. (2019). “Glucose-6 phosphate, A central hub for liver carbohydrate metabolism,” Metabolites, vol. 9, no. 12, Dec. doi: 10.3390/metabo9120282.
[5] J. Zou et al. (2023). “Zuogui-Jiangtang-Qinggan-Fang alleviates high-fat diet-induced type 2 diabetes mellitus with non-alcoholic fatty liver disease by modulating gut microbiome-metabolites-short chain fatty acid composition,” Biomed. Pharmacother., vol. 157, no. November 2022, p. 114002, doi: 10.1016/j.biopha.2022.114002.
[6] Y. Shu, F. Hassan, M. C. Ostrowski, and K. D. Mehta. (2021). “Role of hepatic PKCβ in nutritional regulation of hepatic glycogen synthesis,” JCI Insight, vol. 6, no. 19, pp. 1–15, doi: 10.1172/jci.insight.149023.
[7] B. Cadet, D. Meshoyrer, and Z. Kim. (2021) “Atypical hemolytic uremic syndrome: when pregnancy leads to lifelong dialysis: a case report and literature review,” Cardiovasc. Endocrinol. Metab., vol. 10, no. 4, pp. 225–230, doi: 10.1097/xce.0000000000000247.
[8] T. B. Bennike et al.( 2020). “Preparing for Life: Plasma Proteome Changes and Immune System Development During the First Week of Human Life,” Front. Immunol., vol. 11, no. October, pp. 1–18, doi: 10.3389/fimmu.2020.578505.
[9] Wu, H., Oliveira, G., & Lila, M. A. (2023). Protein‐binding approaches for improving bioaccessibility and bioavailability of anthocyanins. Comprehensive Reviews in Food Science and Food Safety, 22(1), 333-354.
[10] M. Orsaria, A. P. Londero, S. Marzinotto, C. DI Loreto, Di. Marchesoni, and L. Mariuzzi. (2017). “Placental type alkaline phosphatase tissue expression in ovarian serous carcinoma,” Cancer Biomarkers, vol. 17, no. 4, pp. 479–486, doi: 10.3233/CBM-160665.
[11] J. X. Xu et al., “Co-inheritance of G6PD deficiency and 211 G to a variation of UGT1A1 in neonates with hyperbilirubinemia in eastern Guangdong,” BMC Pediatr., vol. 21, no. 1, pp. 1–8, 2021, doi: 10.1186/s12887-021-03010-6.
[12] S. S. Saleh. (2019). “Study the effectiveness of (G-6-phd) enzyme and the level of fats in peoples with leukemia,” Int. J. Pharm. Qual. Assur., vol. 10, no. 3, pp. 142–146, doi: 10.25258/IJPQA.10.3.6.
[13] J. Xu, Y. Yang, A. Jiang, and H. Zhu. (2020). “Critical Reviews in Analytical Chemistry Detection Methods and Research Progress of Human Serum Albumin Detection Methods and Research Progress of Human Serum Albumin,” Crit. Rev. Anal. Chem., vol. 0, no. 0, pp. 1–21, doi: 10.1080/10408347.2020.1789835.
[14] B. T. Doumas. (1975). “Standards for total serum protein assays: a collaborative study,” Clin. Chem., vol. 21, no. 8, pp. 1159–1166, doi: 10.1093/clinchem/21.8.1159.
[15] Ndeh, F. J., Ojong, E. W., Akpan, U. O., & Ekeagba, I. I. (2022). Serum Amino Transaminases Activities and De Ritis Ratio amongst Apparently Healthy Secretors and Non-Secretors of ABH Substances Dwelling in Uyo Urban, Akwa Ibom State, Nigeria. Asian Journal of Research and Reports in Hepatology, 4(1), 1-20.
[16] Rami, L., Roura, M., & Canalias, F. (2012). Evaluation of commutability of several materials for harmonization alkaline phosphatase catalytic concentration measurements. Clinica Chimica Acta, 413(15-16), 1249-1254.
[17] Pearlman, F. C., & Lee, R. T. (1974). Detection and measurement of total bilirubin in serum, with use of surfactants as solubilizing agents. Clinical chemistry, 20(4), 447-453.
[18] M. Tiwari. (2017). “Glucose 6 phosphatase dehydrogenase (G6PD) and neurodegenerative disorders: Mapping diagnostic and therapeutic opportunities,” Genes Dis., vol. 4, no. 4, pp. 196–203. doi: 10.1016/j.gendis.2017.09.001.
[19] M. Scott, L. Zuo, B. Lubin, and D. Chiu. (1991). “NADPH, not glutathione, status modulates oxidant sensitivity in normal and glucose-6-phosphate dehydrogenase-deficient erythrocytes,” Blood, vol. 77, no. 9, pp. 2059–2064. doi: 10.1182/blood.v77.9.2059.bloodjournal7792059.
[20] Prakoeswa, F. R. S., Satria, Y. A. A., Prasetyo, B., Martini, S., Listiawan, M. Y., Endaryanto, A., & Prakoeswa, C. R. S. (2021). Nutritional Status and Blood Profile amongst Patient withChildand Maternal Leprosy in Endemic and Non-Endemic Area of Indonesia. Indian Journal of Forensic Medicine & Toxicology, 15(3), 3010-3017..
[21] E. C. Britt et al.( 2022). “Switching to the cyclic pentose phosphate pathway powers the oxidative burst in activated neutrophils,” Nat. Metab., vol. 4, no. 3, pp. 389–403. doi: 10.1038/s42255-022-00550-8.
[22] M. Z. Hong et al. (2022). “Different clinical features of children and adults in regional outbreak of Delta COVID-19,” BMC Infect. Dis., vol. 22, no. 1, pp. 1–7. doi: 10.1186/s12879-022-07707-6.
[23] Kadiiska, M. B., Hanna, P. M., Jordan, S. J., & Mason, R. P. (1993). Electron spin resonance evidence for free radical generation in copper-treated vitamin E-and selenium-deficient rats: in vivo spin-trapping investigation. Molecular pharmacology, 44(1), 222-227.
[24] Xiao, Y., Lu, J., Chang, W., Chen, Y., Li, X., Li, D., ... & Yang, H. (2019). Dynamic serum alkaline phosphatase is an indicator of overall survival in pancreatic cancer. BMC cancer, 19, 1–8.
[25] O. T. Kayode, D. E. Rotimi, T. D. Olaolu, and O. Stephen. (2020). “Biomedicine & Pharmacotherapy Ketogenic diet improves and restores redox status and biochemical indices in monosodium glutamate-induced rat testicular toxicity,” Biomed. Pharmacother., vol. 127, no. May, p. 110227. doi: 10.1016/j.biopha.2020.110227.
[26] Kamal, A. N., & Hassan, A. F. (2021). Comparative Study of Liver Function and Rh Blood Group between both Physiological and Pathological Neonatal Jaundice. Iraqi Journal of Pharmaceutical Sciences (P-ISSN 1683-3597 E-ISSN 2521-3512), 30(1), 101-109.
[27] Airaodion, A. I., Ngwogu, A. C., Ekenjoku, J. A., & Ngwogu, K. O. (2020). Hepatoprotective potency of ethanolic extract of Garcinia kola (Heckel) seed against acute ethanol-induced oxidative stress in Wistar rats. International Research Journal of Gastroenterology and Hepatology, 3(2), 1-10.
[28] U. Cl. (2017). “Correlates of Steady State Lipid Profile and Anthropometric Parameters in Adult Sickle Cell Anaemia Patients in South-West Nigeria,” IOSR J. Dent. Med. Sci., vol. 16, no. 02, pp. 76–8. doi: 10.9790/0853-1602017681.
[29] Airaodion, A. I., Ene, A. C., Ogbuagu, E. O., Okoroukwu, V. N., Ekenjoku, J. A., & Ogbuagu, U. (2019). Biochemical changes associated with consumption (by rats) of “garri” processed by traditional and instant mechanical methods. Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(4), 1-11.
[30] Uche, C. L., Isaiah, A. O., Ezirim, E. O., & Airaodion, A. I. (2022). Sickle cell anemia contributes to liver abnormality. International Journal of Research and Reports in Hematology, 5(2), 122-134.
[31] Airaodion, A. I., Megwas, A. U., Ekenjoku, J. A., & Ngwogu, K. O. (2020). Nephro-and hepato-toxicity of common household insecticides used in Nigeria. International Research Journal of Gastroenterology and Hepatology, 3(2), 21-28.
[32] Airaodion, A. I., Alabi, O. J., Ogbuagu, E. O., Atiba, F. A., & Olawoyin, D. S. (2020). Nephro and hepatotoxic effect of air-freshener in Wistar rats. Asian Journal of Research in Nephrology, 3(2), 1-9.
[33] Nnachi, O. C., Orih, M. C., Edenya, O. O., Okoye, A. E., & Ezenwenyi, I. P. (2022). Micronutrient levels and haemato-biochemical status of patients with sickle cell anaemia at a tertiary hospital in Abakaliki, south-eastern Nigeria: a cross-sectional study. The Pan African Medical Journal, 41(303), 5-10.
[34] Creeden, J. F., Gordon, D. M., Stec, D. E., & Hinds Jr, T. D. (2021). Bilirubin as a metabolic hormone: The physiological relevance of low levels. American Journal of Physiology-Endocrinology and Metabolism, 320(2), E191-E207.
[35] Jameel, Z. J., Al-Assie, A. H., & Badawy, A. S. (2018). Isolation and characterization of enteroaggregative Escherichia coli among the causes of bacterial diarrhea in children. Tikrit Journal of Pure Science, 20(4), 30-37.
[36] Bakr, A. Y., Marbut, M. M., & AL-Ani, A. H. (2019). Study the relationship between the liver functions and interleukin-10 in female thalassaemia patients in Kirkuk city. Tikrit Journal of Pure Science, 24(2), 1-4.
[37] D. Aydemir and N. N. Ulusu. (2020). “Is glucose-6-phosphate dehydrogenase enzyme deficiency a factor in Coronavirus-19 (COVID-19) infections and deaths?,” Pathog. Glob. Health, vol. 114, no. 3, pp. 109–110. doi: 10.1080/20477724.2020.1751388.
[38] M. M. Loniewska, A. Gupta, S. Bhatia, I. MacKay-Clackett, Z. Jia, and P. G. Wells. (2020). “DNA damage and synaptic and behavioural disorders in glucose-6-phosphate dehydrogenase-deficient mice,” Redox Biol., vol. 28, no. July 2019, p. 101332. doi: 10.1016/j.redox.2019.101332.