Evaluation of the level of 5'-nucleotidase and some biochemical parameters in the sera of beta-thalassemia patients
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Abstract
One of the hydrolytic enzymes that catalyzes the hydrolysis of 5' -nucleotides into ribonucleosides and phosphate is the 5'nucleotidase, which is found in several organs. Oxidative stress shows depletion of antioxidants, such as glutathione (GSH), and the increased concentration of product of lipid peroxidation, such as MDA. The study comprised a total of 54 subjects including patients with beta thalassemia (n=27) and healthy volunteers (n=27) matched by age and gender. Hematology (Hb and % PCV), 5'-nucleotidase, Ferritin, GSH and MDA were measured using different methods and devices. The 5'nucleotidase, Ferritin, and MDA levels were significantly increased (p<0.001) in beta-thalassemia patients compared to the controls. As opposed to that, the Hb, % PCV and GSH levels were significantly decreased (p<0.001) in patients with beta-thalassemia. There was a negative significant correlation (r = -0.146, -0.245, p<0.05) between Hb and 5'- NT enzyme and PCV% with 5'- NT enzyme, respectively. While Hb had a high significant correlation (r = 0.956, p<0.05) with PCV%. In addition, 5'- NT enzyme had a positive significant correlation with GSH, MDA and Ferritin (r = 0.584, 0.442, 0.735, p<0.05), respectively. The present findings indicated an increase in the level of effectiveness of the 5'nucleotidase enzyme, ferritin and GSH in β thalassemia patients while decreasing in MDA.
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References
Adaay, M. H., Al-anzy, M. M., Al-tikriti, K. A., & Mbchb, F. A. A. (2011). Some Observations on the Occurrence of β-Thalassemia in Mosul. Iraqi Journal of Medical Sciences, 8, 270–274. https://www.iasj.net/iasj/article/31437
Ahmed, S. N. (2015). ALTERATIONS IN SERUM 5 ’ NUCLEOTIDASE AND MALONDIALDEHYDE IN BREAST TUMORS. International Journal of Technical Research and Applications, 3(2), 170–173. https://www.ijtra.com/abstract.php?
Al-Mudhaffar, S. A., & Al-Salihi, F. (1980). Kinetics of aspartate aminotransferase fractions from normal human serum. Indian Journal of Biochemistry & Biophysics, 17(2), 127–129. https://europepmc.org/article/MED/7450789
AL-Taii. A. I. Y. (2015). Estimation of 5 ` -nucleotidase activity from saliva of diabetic patients with partial purification of its isoenzymes. Tikrit Journal of Pure Science, 20(2), 105–109. https://doi.org/https://doi.org/10.25130/tjps.v20i2.1167
Bogusławska, D. M., Skulski, M., Bartoszewski, R., Machnicka, B., Heger, E., Kuliczkowski, K., & Sikorski, A. F. (2022). A rare mutation (p.F149del) of the NT5C3A gene is associated with pyrimidine 5′-nucleotidase deficiency. Cellular and Molecular Biology Letters, 27(1). https://doi.org/10.1186/s11658-022-00405-w
Carsote, M., Vasiliu, C., Trandafir, A. I., Albu, S. E., Dumitrascu, M. C., Popa, A., Mehedintu, C., Petca, R. C., Petca, A., & Sandru, F. (2022). New Entity—Thalassemic Endocrine Disease: Major Beta-Thalassemia and Endocrine Involvement. Diagnostics, 12(8), 1–29. https://doi.org/10.3390/diagnostics12081921
David, O., Sacchetti, L., Vota, M. G., Comino, L., Perugini, L., & Pescarmona, G. P. (1990). Pyrimidine 5’-nucleotidase and oxidative damage in red blood cells transfused to beta-thalassemic children. Haematologica, 75(4), 313–318. https://europepmc.org/article/MED/2276676
David, O., Vota, M. G., Piga, A., Ramenghi, U., Bosia, A., & Pescarmona, G. P. (1989). Pyrimidine 5′-nucleotidase
acquired deficiency in β-thalassemia: Involvement of enzyme-SH groups in the inactivation process. Acta Haematologica, 82(2), 69–74. https://doi.org/10.1159/000205286.
Dehghani, M., Karimzadeh, P., Azadeh, N., & Rezvani, A. (2021). Serum ferritin and hematological indices in thalassemia minor and nontransfusion dependent hemoghlobinopathy. Iraqi Journal of Hematology, 10, 17–22. https://doi.org/10.4103/ijh.ijh
Demosthenous, C., Vlachaki, E., Apostolou, C., Eleftheriou, P., Kotsiafti, A., Vetsiou, E., Mandala, E., Perifanis, V., & Sarafidis, P. (2019). Beta-thalassemia: renal complications and mechanisms: a narrative review. Hematology (United Kingdom), 24(1), 426–438. https://doi.org/10.1080/16078454.2019.1599096
Goldberg, D. M. (1973). 5’Nucleotidase: Recent Advances in Cell Biology, Methodology and Clinical Significance. Digestion, 8(1), 87–99. https://doi.org/10.1159/000197303
Huang, K., Ge, S., Yi, W., Bi, H., Lin, K., Sun, H., Huang, X., Chu, J., Ma, S., & Yang, Z. (2019). Interactions of unstable hemoglobin Rush with thalassemia and hemoglobin E result in thalassemia intermedia. Hematology (United Kingdom), 24(1), 459–466. https://doi.org/10.1080/16078454.2019.1621020
Kadir, M. A., El-Yassin, S. T., & AM, A. (2018). Estimation of Nitric Oxide, Malondialdehyde, and Adenosine Deaminase in Serum of Hypertensive Patients and Normotensive Individuals in Erbil City. Tikrit Journal of Pure Science, 23(9), 1813–1662. http://dx.doi.org/10.25130/tjps.23.2018.141
Knight, J. A., Pieper, R. K., & McClellan, L. (1988). Specificity of the thiobarbituric acid reaction: Its use in studies of lipid peroxidation. Clinical Chemistry, 34(12), 2433–2438. https://doi.org/10.1093/CLINCHEM/34.12.2433
LOWRY, O. H., & LOPEZ, J. A. (1946). The determination of inorganic phosphate in the presence of labile phosphate esters. The Journal of Biological Chemistry, 162(3), 421–428. https://doi.org/10.1016/s0021-9258(17)41386-x
Mehsen, J. T., Madhi, Z. S., & Madhi, I. S. (2019). Biochemical Significance of Ecto-5’-Nucleotidase, Xanthine Oxidase, and Glutathione S-Transferase Determinations in Sera of Cigarette and Water Pipe Young Men Smokers. Medical Journal of Babylon, 16(1), 117–121. https://doi.org/10.4103/MJBL.MJBL
Miess, H., Dankworth, B., Gouw, A. M., Rosenfeldt, M., Schmitz, W., Jiang, M., Saunders, B., Howell, M., Downward, J., Felsher, D. W., Peck, B., & Schulze, A. (2018). The glutathione redox system is essential to prevent ferroptosis caused by impaired lipid metabolism in clear cell renal cell carcinoma. Oncogene 2018 37:40, 37(40), 5435–5450. https://doi.org/10.1038/s41388-018-0315-z
Mohammed, K. N., & Marbut, M. M. (2019). Effect of sex class on the levels of some biochemical variables in thalassemia patients. Tikrit Journal of Pure Science, 24(6), 1–9. https://doi.org/http://dx.doi.org/10.25130/tjps.24.2019.101
Nasar, M. A., Subhani, T. F., & Ranjan Sinha, R. (2018). Antioxidant status, Oxidative stress and 5’-Nucleotidase activity in alcoholic and drug induced cirrhotic patients. Journal of Dental and Medical Sciences (IOSR-JDMS),
17(1), 36–43. https://doi.org/10.9790/0853-1701173643
Nickavar, A., Qmarsi, A., Ansari, S., & Zarei, E. (2017). Kidney function in patients with different variants of beta-thalassemia. Iranian Journal of Kidney Diseases, 11(2), 132–137. https://pubmed.ncbi.nlm.nih.gov/28270645/
Pratibha, K., Anand, U., & Agarwal, R. (2004). Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis. Indian Journal of Clinical Biochemistry, 19(2), 128–131. https://doi.org/10.1007/BF02894271
Rathnakumar G, R. A. (2000). Can 5‟-nucleotidase estimation be a predictor of liver metastases? Indian Journal of Cancer, 37(1), 23–26. https://europepmc.org/article/med/11261232
Sedlak, J., & Lindsay, R. H. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Analytical Biochemistry, 25(C), 192–205. https://doi.org/10.1016/0003-2697(68)90092-4
Şen, V., Ece, A., Uluca, Ü., Söker, M., Güneş, A., Kaplan, I., Tan, I., Yel, S., Mete, N., & Sahin, C. (2015). Urinary early kidney injury molecules in children with beta-thalassemia major. Renal Failure, 37(4), 607–613. https://doi.org/10.3109/0886022X.2015.1007871
Srihirun, S., Tanjararak, N., Chuncharunee, S., Sritara, P., Kaewvichit, R., Fucharoen, S., Pattanapanyasat, K., & Sibmooh, N. (2015). Platelet hyperactivity in thalassemia patients with elevated tricuspid regurgitant velocity and the association with hemolysis. Thrombosis Research, 135(1), 121–126. https://doi.org/10.1016/J.THROMRES.2014.10.010
Vyas, S., R.K.Vyas, D., & Sharma, H. (2019). ‘Role of Serum Cholinesterase and 5-Nucleotidase Enzyme Activity in the Diagnosis of Alcoholic and Non-Alcoholic Liver Disease and Compared With Healthy Subjects of Rajasthan.’ Saudi Journal of Medical and Pharmaceutical Sciences, 05(12), 1120–1126. https://doi.org/10.36348/sjmps.2019.v05i12.017
Warang, P., Kedar, P., Ghosh, K., & Colah, R. B. (2012). A new simple approach for the determination of pyrimidine 5′-nucleotidase activity in human erythrocytes using an ELISA reader. International Journal of Laboratory Hematology, 34(3), 232–236. https://doi.org/10.1111/j.1751-553X.2011.01381.x
Weatherall, D. J. (2010). The inherited diseases of hemoglobin are an emerging global health burden. Blood, 115(22), 4331–4336. https://doi.org/10.1182/blood-2010-01-251348
Woolley, Timothy MSc, CSci, FIBMS; Davies, Bethan Jade MSc; Rutter, Emma MSc; Probert, Charlotte; Fitzgerald, Leanne MSc; Relf, W. (2017). A Comparison Between the HORIBA Yumizen H500 Point-of-Care Hematology Analyzer With a 5-Part White Cell Differential and the HORIBA Pentra 120. The Journal of Near-Patient Testing & Technology, 16(2), 89–92. https://doi.org/10.1097/POC.0000000000000133
Yang, H., Chen, Y., Mao, H., Nursing, K. L.-H. li yan jiu=, & 2001, undefined. (n.d.). Illness knowledge, social support and self care behavior in adolescents with beta-thalassemia major. Europepmc.Org. Retrieved March 21, 2023, from https://europepmc.org/article/med/11548457