Study of some Biochemical Parameters and Partial Purification of amylase from diabetic patients
Article Sidebar
Main Article Content
Abstract
The aim of the study was to evaluate the level of amylase in the serum of diabetic patients compared to the level of healthy controls, in glucose, lipids, lipoproteins, thyroid hormones, electrolytes (calcium, potassium, and sodium) and transporter enzymes were also studied. (AST), (ALT),(ALP). the amylase enzyme was purified and the association between the enzyme and the previously mentioned variants was studied. it was studied amylase and other biochemical variables and it was found that there was a significant positive correlation when comparing the enzyme level with the variants (TG, VLDL, ALP) and there was no significant correlation. between enzyme activity and other biochemical variables. Where one peak was obtained in the fourth part of the purification, the degree of purification was 16.1; the result of the enzyme was 108.2% and the specific activity (0.189 ng/ mg, kinetic studies of the partially purified enzyme. The substrat concentration was 10 ng/mg, and the value of Michaelis Menten’s constant km (5.55 ng) and maximum velocity Vmax was (0.98 ng/ml) the optimum temperature of the enzyme were reached (37 °C) while the optimum pH was (7.5).
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
[1] Hamid, Rafah, Razzouk, Hamid, Othman Rashid Thabet, Noor Muhammad, Abdul-Razzaq, Light Essam (2013), A mobile cautery study of liver function in patients with type II disease with arterial hypertension, Anbar University Journal of Pure Sciences. Volume (7), Number (1(
[2] Sultan, a. (2000). "Evaluation of alkaline phosphatase in diabetes", MSC. Thasis University. Libyan.
[3] Muhammad, IAS. (1999) “Liver disease in diabetic patients: a biochemical profile, university thesis, Tikrit, Iraq.
[4] Angulo, Pang; Lindor, KD (2002). Quadrennial review of nonalcoholic fatty liver disease. Journal of Gastroenterology and Hepatology. Supplement 17: S186 - S190.
[5] Richard, PA (2001). “Effect of sodium supplementation on glucose tolerance and insulin concentrations in patients with hypertension and diabetes,” published by Elsevier Science Inc, 14, NO. 7, part 1.
[6] Nabil, A.H., and Abdel Basset, Y. (2016). "Evaluation of electrolyte imbalance and dyslipidemia in patients with diabetes", med. Science 4 (7(
[7] Kelly, G. S. (2000). Insulin resistance: lifestyle and dietary interventions. Med. Rev. 5 (2): pp. 109 - 132.
[8] Safaa A. Wejdan, AR. Nazik, A. Nabila May (2016). Serum calcium levels in association with glycated hemoglobin in Sudanese type 2 diabetic patients, Advances in Diabetes and Metabolism, 4 (4): 59-64.
[9] Nelson, D.L. Cox, M.M. (2000). "Lehninger's Principles of Biochemistry," 3rd ed. New York, USA, p.: 130.
[10] Yassin, Israa Ismail. 2012) “Alkaline phosphate purification