Study the effect of compound Extracted from pomegranate peels and green tea on some biochemical variables in rabbit serum induced with Myocardial Infarction
Main Article Content
Abstract
This study aims to investigate the effect of compounds extracted from pomegranate peels and green tea on some biochemical variables in the serum of domestic male rabbits with recently myocardial infarction.
The study was conducted in the Animal House of the College of Veterinary Medicine. 24 male rabbits were used, with an average of weights ranging between 1800 - 2000 grams, which were divided into three groups (8 / group). The first group was fed a standard diet as a control group and the second and third group fed a standard diet added to it Cholesterol by 1%. After 45 days, the second and third group animals were dosed, in which the myocardial infarction developed the extracted compounds, and the myeloperoxidase enzyme activity and the level of troponin, potassium, and magnesium were measured.
Blood samples were collected from the control groups, the second and the third in which the disease was developed, and after 21 days the samples were collected from the second and third groups after treatment with the extracted compounds. The induction of myocardial infarction led to a significant increase in the activity of myeloperoxidase enzyme MPO and the level of troponin, potassium and magnesium, with a probability level of p≤0.01.
In comparison with control, there was a significant decrease in myeloperoxidase activity and potassium troponin level for groups in which the extracted compounds were dosed after disease onset, and with a probability p≤0.01 compared to the affected groups
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
[ 1 ] Guyton, A.C., and Hall, J.E., )2016(,” text book of medical physiology'' 13th ed., Philadelphia, Pennsylvania. Esevier Inc. PP:1.
[ 2 ] Partha, D., Gabriel, Ying, W., Florian., L., Rostic, G., Clinton , S., et.al. , (2012), Myocardial infraction accelerates atherosclerosis, Nature. 487 (7407): 325-329.
[ 3 ] Roger, S., Joanne, M., and Nathan, D., 2011, '' Preventive Cardiology: A Companion to Braunwald,s Heart Disease'' , 1st ed. Elsevier philadelphia, 1, chap. (2), p:14.
[ 4 ] Fredrika, A., Van, N., Michael, S., Cecil, P., Janus, M., Augustinus, D., et al., (2015), Cardiovascular disease risk. JAMA inter. Medi., 175(6): 1007 – 1017.
[5] Patric , T., Bradshaw , J., Nikhil ,K., Susan , L., Teitelbaum, A., Neugut, d., et al., (2016), Cardiovascular disease mortality among breast Cancer survivors , epidemiology Cambridge , Mass ., 27(1): 6-13.
[6] Lavie, C., McAuley P., Church T., Milani R., Blair, S., (2014), Obesity and cardiovascular diseases: implication regarding fitness, and severity in the obesity paradox. J. Am. Coll. Cardiol., 63 (14): 1345 – 54.
[7] Tortora, G., and Bryan Derrickson, (2011),” principles of Anatomy and physiology”, 13th ed.; Hoboken: Jone Wiley & son.
[8] Barnes, J., (2001), An introduction to herbal medicinal products, Pharmacol. J., 268: 804-806.
[9] Al-Mosawe, E., Al-Saadi, (2012), The Extraction and purification of Gallic acid from the pomegranate rind, Al-Mustansiriyah Jour. Sci. 23(6):53-60.
[10] Harborn, J., (1998)” Phytochemical Method” 3rd ed. Chapman &Hall.
[11]-Al-Jumaily, E., Al-abdli, Y., Nasser, N., (2011), Extraction of catechin from green tea plants camellia sinensis, Journal of Biotechnology Research Center, 5(3):9-14.
[12] National Research Council. (1994)” Nutrient requirements of poultry” 9th revised, National academy press, Washington. D. C.
[13] Mohamed, A., Abdelhalim, N., et al. (2008), effects of feeding periods of high cholesterol and saturated fat diet on blood biochemistry and hydroxyproline fraction in rabbits, Bio. Biol. Insights. 2:95-100.
[14]-Choudhury, R., Srai, S., et al. (1999), Urinary excretion of hydroxycinnamates and flavonoids after oral and intravenous administration, Free Radical Biology and Medicine, 27(3-4):278-289.
[15] Bogdanski, P., et al., (2012),Green tea extract reduced blood pressure, inflammatory biomarker, and oxidative stress and improves parameters associated with insulin resistance in obese, hypertensive patients, Nut. Res. 32(6):421-427.
[16] Lundquist, I., Josefsson, J., (1971), Sensitive method for determination of peroxidase activity in tissue by means of coupled oxidation reaction, Analytical Biochemistry. 41(2):567-577.
[17] Xia, Y., Zweier, J., (1997), Measurement of myeloperoxidase in leukocyte-containing tissues, Analytical Biochemistry., 245(1):93 -96.
[18] Wong, S., (1996), Strategic utilization of cardiac markers for diagnosis of acute myocardial infarction, Annals of Clinical and Laboratory Science Journal, 26(4):301 -312.
[19] Hilliman, G., et al. (1967), Rapid determination of serum potassium by turbidity measurement with kalignost after protein Precipation, Z kiln. Chem. Klin. Biochem. 5(2):93-94.
[20] Thomas, L., (1998), “Clinical laboratory diagnostic” 1st ed. Frankfurt. Th-Books Verlagsgesellshaft, pp: 231-241.
[21] Mann, C., You, J., (1957), Spectrophotometric determination of Mg Anal. Chem. Acta.16:155-60.
[22] Yuan, C., Jin, Z., et al. (2012), Inclusion complex of astaxanthin with hydroxypropyl-B-cyclodextrin: UV, FTIR, 1HNMR and molecular modeling studies, 89:492-496.
[23] Zhang, J., Li, C., et al. (2011) controllable exploding microcapsules as drug carriers, Chem. Cmmun. 47:4457-4459.
[24] Abri, A., & Maleki, M., (2016), Isolation and identification of gallic acid from the elaeagnus angustifolia leave and determination of total phenolic, flavonoids contents and investigation of antioxidant activity, Inter. Chem. Comm. 4:146-154.
[25] Ferenc, B., Ziegler, M., et al. (2007), Vibrational spectroscopic study on the quantum chemical model and the X- ray structure of gallic acid, solvent effect on the structure and spectra, Vibrational Spectroscopy, 43(1):193-202.
[26] Xu, H., Liu, T., et al. (2019), Interactions between B- cyclodextrane and catechins, and potential anti-osteoclastogenesis activity of the (-)-epigallocatechin-3-gallate –B-cyclodextrane complex, RSC. Advance, 9(48):28006-28018.
[27] Ponnuraj, R., et al. (2015), Formulation and characterization of epigallochatechin gallate nanoparticles, Indo Amer. Jour. Pharm. Rec. 5:387-399.
[28] Tian, R., Ding, Y., et al. (2017), Inhibition of myeloperoxidase and neutrophil-mediated hypochlorous acid formation in vitro and endothelial cell injury by (-)-epigallocatechin gallate, Jour. Agric. Food Chem. 65(15):3198-3203.
[29] Yeoh, B., Olvera, R., et al. (2016), Epigallocatechin-3-gallate inhibition of myeloperoxidase and its counter-regulation by dietary iron and lipocalin 2 in murine model of gut inflammation, Am. J. Pathol. 186(4):912-926.
[30] Kroes, B., et al. (1992), Anti-inflammatory activity of gallic acid, Plant. Med. 58(06):499-504.
[31] Perron, N., et al. (2009), A review of the antioxidant mechanisms of polyphenol compounds
related to iron binding, Cell, Bio. Biophys. 53(2):75-100.
[32] Priscilla, D., et al. (2009), Cardioprotective effect of gallic acid troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in wistar rats. Chem. Biol. Interact. 179(2-3):118-124.
[33] Kelemen, K., et al. (2007), green tea flavonoid epigallocatechin-3-gallate (EGCG) inhibits cardiac hERG potassium channels, Bio. & Biophs. Rese. Comm., 364(3):429-435.
[ 34 ] Sora, J., et al (2010), European resuscitation guidelines for resuscitation 2010 section 8. Cardiac arrest in special circumstance: electrolyte abnormalities, poisoning, drowning, accidental hypothermia, hyperthermia, asthma, anaphylaxis, cardiac surgery, trauma, pregnancy, electrocution, Resuscitation, 81(10):1400-1433.