Detection of gene stn in some non-typhoidal Salmonella spp. which isolated from patients with diarrhea
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The present study was conducted to estimate the prevalence of Non-typhoidal Salmonella spp. From April 2017 to September 2017 approximately 222 samples from diarrhoeic stool of human ages between one month till above sixty years were collected in Rizkary , children's and Kirkuk hospitals to determine the prevalence stn gene among Non-typhoidal Salmonella spp(S. enteritidis). A total of 220 samples were collected, The colonial morphology, staining, microscopical examination, cultural media as well as biochemical characteristics of the isolates found out the presence (72) 32.7% out of 220 salmonella species isolates, (41) 57% were belong to NTS from 72 isolates while S. enteritidis were 28(68.2%) from 41 isolates of NTS. PCR assay was carried out to detect the presence of stn gene, (24) 85.7% isolates of S. enteritidis contained the gene among 41 isolates of S. enteritidis. 100% of isolates were susceptible to Norfloxacin, while 16.7% were sensitive to Cefotaxime, 70% of isolates appeared multidrug resistance. 32.1% infections recorded in ages above sixty,25% age less than one year.
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