Isolation and diagnosis of Streptococcus mutans and Streptococcus sorbinus from patients with tooth decay and gingivitis
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Abstract
The Streptococcus bacteria Streptococcus sobrinus and Streptococcus mutans are one of the most common types in the mouth, where tooth decay, necrosis and gingivitis are among the health problems that the Iraqi society suffers from, as the treatment of bacteria has become a challenge due to their ability to resist many antibiotics. The study aimed to isolate and diagnose Streptococcus mutans and Streptococcus sobrinus and study their resistance to a number of antibiotics, and evaluate the susceptibility of Streptococcus mutans and Streptococcus sobrinus isolates in biofilms. Using biochemical tests and phenotypic diagnostics. The study included the collection of (275) oral swabs from patients with tooth decay from the area of caries on the surface of the tooth and from cysts for infected patients and visitors at the Medical City Hospital in Baghdad Governorate with ages ranging between (6-70) years and of both sexes for the period from 1/9/2022 to 1/11/2022. the number of isolates was 6 (2.18%) belonging to Streptococcus mutans and only one isolate (0.36%) of Streptococcus sobrinus, the sensitivity of all the isolates of the bacteria under study was tested towards 10 antibiotics, and the results showed that the isolates were resistant to most of the antibiotics used in this study, and all the isolates were chosen to test their ability to form biofilms using the microtitration plate, and the results showed that most of the isolates were strong in the formation of biofilms.
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References
[1] Garcke, H. and Nürnberg, R. (2021). Structure-preserving discretizations of gradient flow for axisymmetric two-phase biomembranes. IMA Journal of Numerical Analysis, 41(3): 1899-1940. [2] Elgamily, H. M.; Gamal, A. A.; Saleh, S. A.; Wahab, W. A. A.; Hashem, A. M.; and Esawy, M. A. (2019). Microbiological and environmental assessment of human oral dental plaque isolates. Microbial pathogenesis, 135: 103626. [3] Poza-Pascual, A., Serna-Muñoz, C., Pérez-Silva, A., Martínez-Beneyto, Y., Cabello, I., & Ortiz-Ruiz, A. J. (2021). Effects of Fluoride and Calcium Phosphate-Based Varnishes in Children at High Risk of Tooth Decay: A Randomized Clinical Trial. International journal of environmental research and public health, 18(19): 10049. [4] Taketo. K.; Naoki, N.; Saori,Y.; Yoshiaki, T.; Jun. I.; Yasutaka. H.; Hidenobu. S.(2016). Inhibition of Streptococcus mutans biofilm formation. [5] Lemos, J. A.; Palmer, S. R.; Zeng, L.; Wen, Z. T.; Kajfasz, J. K.; Freires, I. A.; Abranches, J. and Brady, L. J. (2019). The Biology of Streptococcus mutans. Microbiology spectrum, 7(1), 10.1128/microbiolspec.GPP3-0051-2018 . [6] Matsumoto-Nakano. (2018). Role of Streptococcus mutans surface proteins for biofilm formation Japanese Dental Science Review . [7] Salehi,B.; Kregiel,D.; Mahady.G.; Sharifi-Rad.J.; Martins.N.; Rodrigues.C . (2020). Management of Streptococcus mutans-Candida spp. Oral Biofilms’ Infections: Paving the Way for Effective Clinical Intervention. J Clin . 9(2): 517. [8] Sales, MJ.; Herbert, WG.; Du, Y.; Sandur, AS.; Stanley, NM.; Jensen, PA. (2018). Complete genome sequences of Streptococcus sobrinus SL1 (ATCC 33478 = DSM 20742), NIDR 6715-7 (ATCC 27351), NIDR 6715-15 (ATCC 27352), and NCTC 10919 (ATCC 33402). Microbiol Resour Announc 7:e00804-18. [9] Abd, Suha T., and Abbas F. Ali. (2016) "The Effect of Zinc Oxide Nanoparticles on Streptococcus mutans of Human Saliva (In Vitro Study)." Journal of baghdad college of dentistry 28.2: 158-164. [10] Nagamine, Y., Hasibul, K., Ogawa, T., Tada, A., Kamitori, K., Hossain, A., and Miyake, M. (2020). D-Tagatose Effectively Reduces the Number of Streptococcus mutans and Oral Bacteria in Healthy Adult Subjects: A Chewing Gum Pilot Study and Randomized Clinical Trial. Acta Medica Okayama, 74(4), 307-317. [11] MacFaddin, J.F. (2000). Biochemical test for identification of medical bacteria .Lippincott Willims and Wilkins. Philadelphia, USA. [12] Forbes, B.A. ; Sahm, D.F. ; and Weissfeld, A.S. (2007). Bailey and Scott's diagnostic microbiology. 12th ed, evolve, china. [13] Al-Mudallal, N. H., Al-Jumaily ,E. F., Muhimen. N. and Al-Shaibany A. A.(2008). Isolation and Identification of Mutans Streptococci Bacteria from Human dental Plaque samples. Journal of Al-Nahrain University Vol.11(3), pp.98-105.
[14] Levinson, W. (2016). Review of Medical Microbiology and Immunology. 14thed. McGraw-Hill education, Inc. PP 821. [15] Wanger, A.; Chavez, V.; Huang, R.; Wahed, A.; Dasgupta, A. and Actor, J.K. (2017). Microbiology and Molecular Diagnosis in Pathology: A Comprehensive Review for Board Preparation, Certification and Clinical Practice. Elsevier 285. [16] Brooks, G.F.; Carroll, K.C; Butel , J.S.; Mores, S.A. and Mietzner, T.A. (2018). Jawetz , Melnick and Adelbergs Medical Microbiology .25th ed. thr McGraw-Hill companies ,United states of American . [17] Leber, A.L.(2016).Clinical Microbiology Procedures Handbook, 4th ed, vol 2.Washington DC: ASM Press. [18] Harvey, R.A.; Cornelissen, C.N. and Fisher, B.D. (2013). Lippincott's Illustrated Review Microbiology. 3d ed. Lippincott Williams and Wilkins Wolters Kluwer business. USA . [19] Bauer, A. T. (1966). Antibiotic susceptibility testing by a standardized single disc method. Am J clin pathol, 45, 149-158. [20] Clinical and Laboratory Standards Institute (CLSI). (2015). Performance Standards foAntimicrobial Susce-ptibility Testing; Twenty-Fifth Informational Supplement. Approvedstandard M100-S25. CLSI,Wayne, PA. [21] Kirmusaoglu, S. (2019). The Methods for Detection of Biofilm and Screening Antibiofilm Activity of Agents. In ExopolysaccharidesMethods of Preparation and Application. In technology Open. DOI: http:// dx.doi.org / 10.5772 / intechopen.84411 [22] Flayyih, A.S.; Hassani, H.H. and Wali,M.H. (2016). Identfication of Streptococcus mutans from human dental plaque and Dental caris using 16SrRNA gene, Iraqi J. Of science; 57 (1C): 552-557. [23] Mozaal,H and Chlelab,R .(2019). Isolation and Iodenfication of Streptococcus mutans from oral infection and testing their sensitivity to some plant extractions. Maaysan Research Journal, 14 (28):256. [24] Pisarska,A.; Wolinowska,R.; Rudnicka,J.; Iwanicka-Grzegorek,A. (2022). Characteristics of Clinical Isolates of Streptococcus mutans. J. MDPI Applied Sciences. 12(9), 4579. [25] Zeng,Y.; Fadaak,A.; Alomeir,N.; Wu,Y .; Wu,T.; Qing,S.; Xiao,J. (2023). Effect of Probiotic Lactobacillus plantarum on Streptococcus mutans and Candida albicans Clinical Isolates from Children with Early Childhood Caries. Int J Mol Sci. 24(3): 2991. [26] Saleh, M.; Abd Al-Zahra, H. (2019). Isolation and identification of Streptococcus mutans from dental caries patients at Thi-Qar province/Iraq. University of Thi-Qar Journal of Science, 6(4), 22–27. [27] Zadeh, E.T.; Aref, P.; Askarizadeh, N.; Emadi F.(2023) In vitro Antimicrobial Effect of Punica granatum Extract versus Chlorhexidine on Streptococcus sobrinus, Streptococcus sanguinis, and Candida albicans. J Res Dent Maxillofac Sci. .8(1):18-27. [28] Abranches,J.; Zeng,L.; Kajfasz,J, et al. (2018). Biology of oral Streptococci, Mi crobiol. Spectr.; 6(5) : 1-18. [29] Hussein,Sh.(2020). Study of the diagnosis and isolation of bacteria associated with dental caries in pregnant women in Baghdad provice, EurAsian J . of Bio Sciences; 14 (1) : 1-7 . [30] Abo Bakr, R.; Tawfick, M.; Mostafa, Z.; Abdulal,l A.(2022). Prevalence and Antibiogram of Streptococcus mutans in Dental Plaque and Caries Samples. Azhar International Journal of Pharmaceutical and Medical Sciences. 2022; 2 (2): 83-93, doi: 10.21608/AIJPMS.2022.87671.1084
[31] Salh,A .; Risan,M . and Jasim,H . (2022). Biochemical characteristics and antibiotics susceptibility of Streptococcus mutans isolates from dental caries in baghdad city, International J. of Advanced Biological and Biomedical Research; 10 ,Issue 1: 32-34. [32] Maasi,G.; Štšepetova,J.; Jõesaar,M.; Olak,J. and Mändar,R. (2022). Different Patterns of Virulence Genes in Streptococcus mutans and Streptococcus sobrinus Originating from Estonian Toddlers—Mothers Cohort. Microbiol. Res. 13(4), 928:936. [33] Saleh,B.H.; Ibrahim,R.N.; Al-Ugaili,D.N. (2023). The Effect of Diode Laser on Viability and Antibiotic Sensitivity of StreptococcusmutansIsolated From Dental Caries. Iraqi Journal of Science. Vol. 64, No. 2, pp: 583-593DOI: 10.24996/ijs.2023.64.2.8 [34] Aabdl-Rahimea,A.M and Saleh.N.F .(2017). Evaluation of some antibiotics resistance of different oral bacteria types isolated from patients with gingivitis and periodontitis. University of Thi-Qar Journal Of Science (UTsci). Volume 6, Number 3.
[35] Bachmeier,E.; Goitea,M.E.; Linares,J.; Wietz,F.M.; Jarchum,S.; Jarchum,G.; Brunotto,M.; Mazzeo,M. (2021). Determination of Streptococcus sp. and Candida albicans in the oral cavity of patients undergoing bone marrow transplantation. . Journal of Oral Research. Vol(10) Nº. 6. págs. 1-10. [36] Salman, H.A .; Senthikumar,R . (2015). Identification and Antibiogram Profile of Streptococcus mutans and Streptococcus sobrinus from Dental Caries Subjects. Journal of Applied Pharmaceutical Science. Vol.5 (06), pp. 054-057 [37] OECD, Safety Assessment of Transgenic Organisms, Volume 4: OECD Consensus Documents, 2010, pp.171-174. [38] Zayed,S,M.; Aboulwafa,M.M..; Hashem,A.M and Saleh,S.S .(2021). Biofilm formation by Streptococcus mutans and its inhibition by green tea extracts, AMB Express.; 11: 73. doi: 10.1186/s13568-021-01232-6. [40] Alhasani,A.H.; Ishag,R.A.; Al-Akwa,A.A.; Al Shamahy,H.A.W.; M.A . (2020). Association between the streptococcus biofilm fromation and dental caries experience and antibiotics resistance in adult females. Universal Journal of Pharmaceutical Research; 5(6):18-23.