Molecular detection of Helicobacter pylori isolated from biopsies of gastritis patients based on the presence of 16srRNA and VacA S1/S2-UreA virulence genes
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Abstract
Background: Although most infections with Helicobacter pylori are asymptomatic, some people with this bacterium end up with chronic gastritis, peptic ulcers, or even stomach cancer. The study's objectives wasto detectthe prevalence of Helicobacter pylori by identifying 16sRNA and to determine the virulence genes (ureA and vacAS1/S2) in biopsies of patients with gastritis.
Method: The study was conducted between September 2022 and June 2023 at the public hospital and several private clinics in Balad City, Iraq. A total of 50 samples were taken, spanningfrom 15-85 years of age (50 patients). Balad General Hospital and a private upper surgical endoscopy clinic. The specialist doctor (gastroenterologist) took the gastric biopsy samples from the stomach's antrum and corpus during normal endoscopy. The gastric biopsies were used for PCR.
Results: The current study includes 50 samples suffering from gastritis. Patients according to age groups 26-34 and 45-54 had the highest frequency, while, 55-64 had the lowest frequency. Helicobacter Pylori was detected by16SrRNA. According to the result 16SrRNA by, the research found that positive gastric patients was42 (84%) and 8(16%) negative. In addition, this research found that the UreA gene was39 (78%) positive and 11(22%) negative and theVacS1/S2 gene was22 (44%) positive and 28(56%) negative.
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References
1. Pegu, S.R.R., Rajkhowa, S., Das, P.J., Sonowal, J., Sengar, G.S., Deb, R., Choudhury, M., Deka, N., Paul, S., Doley, J. and Sarma, D.K., 2023. Incidences of Helicobacter infection in pigs and tracing occupational hazard in pig farmers. bioRxiv, pp.2023-03. 2. Canzian, F., Rizzato, C., Obazee, O., Stein, A., Flores‐Luna, L., Camorlinga‐Ponce, M., Mendez‐Tenorio, A., Vivas, J., Trujillo, E., Jang, H. and Chen, W., 2020. Genetic polymorphisms in the cag pathogenicity island of Helicobacter pylori and risk of stomach cancer and high‐grade premalignant gastric lesions. International journal of cancer, 147(9), pp.2437-2445. 3. Faass, L., Stein, S.C., Hauke, M., Gapp, M., Albanese, M. and Josenhans, C., 2021. Contribution of Heptose Metabolites and the cag Pathogenicity Island to the Activation of Monocytes/Macrophages by Helicobacter pylori. Frontiers in Immunology, 12, p.632154. 4. Bustos-Fraga, S., Salinas-Pinta, M., Vicuña-Almeida, Y., de Oliveira, R.B. and Baldeón-Rojas, L., 2023.Prevalence of Helicobacter pylori genotypes: cagA, vacA (m1), vacA (s1), babA2, dupA, iceA1, oipA and their association with gastrointestinal diseases. A cross-sectional study in Quito-Ecuador. BMC gastroenterology, 23(1), pp.1-10. 5. Khelaifia, S., Virginie, P., Belkacemi, S., Tassery, H., Terrer, E. and Aboudharam, G., 2023. Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques. Microorganisms, 11(4), p.836. 6. Săsăran, M.O., Meliț, L.E. and Dobru, E.D., 2021.MicroRNA modulation of host immune response and inflammation triggered by Helicobacter pylori. International Journal of Molecular Sciences, 22(3), p.1406.
7. Altobelli, A., Bauer, M., Velez, K., Cover, T.L. and Müller, A., 2019. Helicobacter pylori VacA targets myeloid cells in the gastric lamina propria to promote peripherally induced regulatory T-cell differentiation and persistent infection. MBio, 10(2), pp.10-1128. 8. Ansari, S. and Yamaoka, Y., 2020. Helicobacter pylorivirulence factor cytotoxin-associated gene A (CagA)-mediated gastric pathogenicity. International journal of molecular sciences, 21(19), p.7430. 9. Schröder, D., Müller, F., Heesen, G., Hummers, E., Dopfer-Jablonka, A., Vahldiek, K., Klawonn, F., Steffens, S., Mikuteit, M., Niewolik, J. and Heinemann, S., 2023. Feasibility of self-organized blood sample collection in adults for study purposes in a primary care setting. Plos one, 18(5), p.e0286014. 10. Hussein, R.A., Al-Ouqaili, M.T. and Majeed, Y.H., 2021. Detection of Helicobacter Pylori infection by invasive and non-invasive techniques in patients with gastrointestinal diseases from Iraq: A validation study. Plos one, 16(8), p.e0256393. 11. Kagihara, J., Matsuda, B., Young, K.L., Li, X., Lao, X., Deshpande, G.A., Omata, F., Burnett, T., Lynch, C.F., Hernandez, B.Y. and Kuwada, S.K., 2020. Novel association between Helicobacter pylori infection and gastrointestinal stromal tumors (GIST) in a multi-ethnic population. Gastrointest. Stromal Tumor, 3(1).
12. Abdulrahman, A.T., Hussain, S.S., Ahmed, N.J. and Hassan, A.N., 2022. Detection of Helicobacter Pylori’sVirulence Gene (UreA) and its Influence on the Result of Rapid Urease Test (RUT). Al-Mustansiriyah Journal of Science, 33(4), pp.42-48.
13. Njenga, P., Njau, A., Moloo, Z., Revathi, G., Tshibangu, E. and Yamaoka, Y., 2023. Pattern and trends of Helicobacter pylori genotypes in gastric cancer: A Kenyan 8-year study. Frontiers in Medicine, 10, p.1119513 14. Majeed, P.D. and Khoshnaw, K.J.S., 2020. Seroprevalence of Helicobacter pylori infection among patients with gastroduodenal disorders in Erbil city. Diyala Journal of Medicine, 18(1), pp.91-101.
15. Almashhadany, D. A. (2020). Epidemiology of Helicobacter Pylori amonhuman at Erbil Governorate/Kurdistan region/Iraq. World Journal ofPharmacy and Pharmaceutical Sciences, 9(11), 435-447 16. Almashhadany, D.A., Mayas, S.M. and Ali, N.L., 2022.Isolation and identification of Helicobacter pylori from raw chicken meat in Dhamar Governorate, Yemen. Italian Journal of Food Safety, 11(2).
17. Zhu, Y.; Zhou, X.;Wu, J.; Su, J. and Zhang, G. (2014). Risk factors and prevalence of Helicobacter pylori infection in persistent high incidence area of gastric carcinoma in Yangzhong city. Journal Gastroenterology Research and Practice.
18. Graham, D. Y. (2000). Helicobacter pylori infection is the primary cause of gastric cancer. Journal of gastroenterology, 35 19. Ahmed , K. S.; Madompoyil , B.; Ghebremedhin , A. A.; Issac, J. ; Ahi ,J. D.; Khan , A. A.; Tiwari, S. K. 2013 . Studying the Importance of VacA Gene of Helicobacter pylori in Identifying the Pathogenicity of Strains by Comparing It with the Disease Status of the Subjects . Journal of Cancer Therapy, 4: 68-74. 20. Ali, S.F. 2013. Detection of Helicobacter pylori in saliva from some Iraqi patients in comparison with other methods. M.Sc thesis, College of Science, Baghdad University. 21. Smith, S.M.; O’Morain, C. and Namara, D .M. 2014.Antimicrobial susceptibility testing for Helicobacter pyloriin times of increasing antibiotic resistance. World J Gastroenterl, 20(29): 9912-9921. 22. Mamoun, M.; Elsanousi, S. ; Khalid, A.; Abdelmounem E. and Mohamed A. 2015. Molecular Identification 0f 16s Ribosomal RNA Gene of Helicobacter pylori Isolated from Gastric Biopsies in Sudan. American Journal of Microbiological Research, 3(2): 50-54. 23. Chiquet, C., Bron, A.M., Lundström, M. and Maurin, M., 2022. Acute postoperative endophthalmitis: Microbiology from the laboratory to the bedside. Survey of Ophthalmology. 24. AlObaidi, A., Alquraishi, Z.H.O. and Alsadawi, A.A., 2022. Bassociation among Biofilm Formation and Antibiotic Resistance Genes in Klebsiella PneumoniaeIsolates from Urinary Tract Infections. HIV Nursing, 22(2), pp.1336-1340. 25. .Bouhenni, H., 2023. Study of combined effect of some medicinal plants and probiotics against Helicobacter pylori responsible for gastroduodenal diseases (Doctoral dissertation, Faculty of Nature and Life Sciences)
26. .Sachs G., Expression of Helicobacter pylori urea I gene required for acidic PH activation ofcytoplasm urease, Infect. Immune. 68, 470-477 (2000). 27.Bibi, F., Alvi, S.A., Sawan, S.A., Yasir, M., Sawan, A.JimaFatani, A.A. and Azhar, E.I., 2017. Detection and genotyping of Helicobacter pylori among gastric ulcer and cancer patients from Saudi Arabia. Pakistan journal of medical sciences, 33(2), p.320.
28.Quezado, M., Cavalcante, D. F., Igor, C., Silva, S., Braga-neto, M. B.,Bessa, A., Fialho, C., Fialho, A. N., Barbosa, A. M. C., Will, F., Cruz,
S., Rocha, G. A., Maria, D., Queiroz, M. De, Libanez, L., & Campelo,
B. (2012). Helicobacter pylori vacA and cagA genotypes in patientsfrom northeastern Brazil with upper gastrointestinal diseases.
107June), 561–563.
29. Segar R.K.2007) Pathogenic and molecular study on Helicobacter pylori isolated from patients of gastric and duodenal ulcers. Ph.D. Thesis Al-Mustansiriyah University. Iraq