Evaluation of Geh gene expression in Staphylococcus aureus isolated from acne patients
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Abstract
Acne vulgaris is a chronic skin disease that affects the hair follicle and sebaceous glands. Staphylococcus aureus colonizes human skin and causes infections ranging from local skin infections to systemic infections that may threaten human life due to its ability to produce many virulence factors and antibiotic resistance. The objective of this study is to measure the genetic expression of Geh gene responsible for producing the lipase enzyme from S. aureus isolated from acne patients. This study was conducted by collecting 130 swabs from acne patients at Tikrit Teaching Hospital. Using routine bacterial isolation and identification methods, 50 samples (38.4%) of the total samples were identified as S. aureus through its ability to grow in mannitol salt agar medium, the fermentation of mannitol sugar, and the change of the medium color to yellow. The Geh gene expression of ten S. aureus isolates from acne patients was examined using RT-PCR. The results showed that Geh gene was expressed in all isolates, with an expression ranging from 1.5 to 7.2, and that the mean folding of the Geh gene for ten samples was 4.2. This indicates the presence of high gene expression of the Geh gene in S. aureus isolates. Therefore, it is concluded that Lipase enzyme plays an important role in the pathogenesis of S. aureus in acne patients.
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References
[1] Lichtenberger, R., Simpson, M. A., Smith, C., Barker, J., & Navarini, A. A. (2017). Genetic architecture of acne vulgaris. Journal of the European Academy of Dermatology and Venereology, 31(12), 1978-1990. https://doi.org/10.1111/jdv.14385
[2] Ramadani, A. H., Karima, R., & Ningrum, R. S. (2022). Antibacterial activity of pineapple peel (Ananas comosus) eco-enzyme against acne bacterias (Staphylococcus aureus and propionibacterium acnes). Indonesian Journal of Chemical Research, 9(3), 201-207. https://doi.org/10.30598//ijcr.2022.9-nin
[3] Masalha M, Borovok I, Schreiber R, Aharonowitz Y, Cohen G (December 2001). "Analysis of transcription of the Staphylococcus aureus aerobics class Ib and anaerobic class III ribonucleotide reductase genes in response to oxygen" Journal of Bacteriology. 183 (24): 7260–72 https://doi.org/10.1128/jb.183.24.72607272.2001
[4] Cheung, G. Y. C., Bae, J. S., & Otto, M. (2021). Pathogenicity and virulence of Staphylococcus aureus. Virulence, 12(1), 547–569. https://doi.org/10.1080/21505594.2021.1878688
[5] Hu, C., Xiong, N., Zhang, Y., Rayner, S., & Chen, S. (2012). Functional characterization of lipase in the pathogenesis of Staphylococcus aureus. Biochemical and biophysical research communications, 419(4),617-620. https://doi.org/10.1016/j.bbrc.2012.02.057 [6] Chen, X., & Alonzo III, F. (2019). Bacterial lipolysis of immune-activating ligands promotes evasion of innate defenses. Proceedings of the National Academy of Sciences, 116(9), 3764-3773. https://doi.org/10.1073/pnas.1817248116
[7] Cadieux, B., Vijayakumaran, V., Bernards, M. A., McGavin, M. J., & Heinrichs, D. E. (2014). Role of lipase from community-associated methicillin - resistant Staphylococcus aureus strain USA300 in hydrolyzing triglycerides into growth-inhibitory free fatty acids. Journal of bacteriology, 196(23),4044-4056. https://doi.org/10.1128/jb.02044-14
[8] Nowicka, D., & Grywalska, E. (2019). Staphylococcus aureus and host immunity in recurrent furunculosis. Dermatology, 235(4),295-305. https://doi.org/10.1159/000499184
[9] Felipe, V., Morgante, C. A., Somale, P. S., Varroni, F., Zingaretti, M. L., Bachetti, R. A., ... & Porporatto, C. (2017). Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms. Microbial pathogenesis, 104, 278-286. https://doi.org/10.1016/j.micpath.2017.01.047
[10] Delekta, P. C., Shook, J. C., Lydic, T. A., Mulks, M. H., & Hammer, N. D. (2018). Staphylococcus aureus utilizes host-derived lipoprotein particles as sources of fatty acids. Journal of bacteriology, 200(11), 10-1128. https://doi.org/10.1128/jb.00728-17
[11] AL-Juhaishy, M. G., & Husain, A. A. (2019). Optimization of L-Glutaminase Enzyme Production by Staphylococcus Aureus Clinical Samples. Indian Journal of Public Health Research & Development, 10(8). 10.5958/0976-5506.2019.02224.1
[12] Ahmad, M. F. and Abas, H. M. 2014. Effect of some amino acids on biofilm for Staphylococcus aureus. Diyala Agricultural Sciences Journal, 6(2):27-38. http://148.72.244.84:8080/xmlui/handle/xmlui/9819
[13] Smyth, R. W., & Kahlmeter, G. (2005). Mannitol salt agar-cefoxitin combination as a screening medium for methicillin-resistant Staphylococcus aureus. Journal of clinical microbiology, 43(8),3797-3799. https://doi.org/10.1128/jcm.43.8.3797-3799.2005 [14] Munson, E., Block, T., Basile, J., Hryciuk, J. E., & Schell, R. F. (2007). Mechanisms to assess Gram stain interpretation proficiency of technologists at satellite laboratories. Journal of clinical microbiology, 45(11),3754-3758 https://doi.org/10.1128/jcm.01632-07
[15] Becker, P., Hufnagle, W., Peters, G. and Herrmann, M. 2001. Detection of differential gene expression in biofilm-forming versus planktonic populations of Staphylococcus aureus using micro-representational-difference analysis. Appl. Environ. Microbiol., 67(7): 2958-2965. https://doi.org/10.1128/AEM.67.7.2958-2965.2001
[16] Schneider, M. R., & Paus, R. (2010). Sebocytes, multifaceted epithelial cells: lipid production and holocrine secretion. The international journal of biochemistry & cell biology, 42(2),181-185 https://doi.org/10.1016/j.biocel.2009.11.017 [17] Gundogan, N., & Ataol, O. (2013). Biofilm, protease and lipase properties and antibiotic resistance profiles of staphylococci isolated from various foods. African Journal of Microbiology Research, 7(28), 3582-3588. DOI: 10.5897/AJMR2012.2316
[18] Rollof, J., Braconier, J. H., Söderström, C., & Nilsson-Ehle, P. (1988). Interference of Staphylococcus aureus lipase with human granulocyte function. European Journal of
Clinical Microbiology and Infectious Diseases, 7,505-510 https://doi.org/10.1007/BF01962601
[19] Rosenstein, R., & Götz, F. (2000). Staphylococcal lipases: biochemical and molecular characterization. Biochimie, 82(11),1005-1014 https://doi.org/10.1016/S0300-9084(00)01180-9.
[20] Atshan, S. S., Shamsudin, M. N., Karunanidhi, A., van Belkum, A., Lung, L. T. T., Sekawi, Z., ... & Hamat, R. A. (2013). Quantitative PCR analysis of genes expressed during biofilm development of methicillin resistant Staphylococcus aureus (MRSA). Infection, genetics and evolution, 18,106-112. https://doi.org/10.1016/j.meegid.2013.05.002