Effect of grape seed and flaxseed oils on kidney and testicular tissue in male rabbits exposed to tramadol-induced oxidative stress
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Abstract
The study aimed to determine how tramadol affects oxidative stress and how flaxseed Oil (FSO) and grape seed oil (GSO) can enhance the biological functions of testicular and renal tissues in male rabbits exposed to tramadol-induced oxidative stress. The experiment was divided into four groups, each comprising five rabbits, with rabbits randomly assigned to groups. Findings: Tramadol significantly increased malondialdehyde (MDA) levels and decreased glutathione (GSH) levels (P<0.05). On the other hand, GSH levels significantly increased (P<0.05), and MDA levels decreased (P<0.05) in groups treated with (FSO) and (GSO). Histological examination of G1 showed stages of spermatogenesis; G2, the stages of sperm formation, are incomplete, with an observed proliferation of sperm-forming cells without the formation of mature sperm. Group G3 shows the stages of sperm formation and mature sperm in their natural state. Group G4 shows the stages of sperm formation and mature sperm in their natural state. In male rabbit kidney tissue, in group (G1), the renal corpuscle, the area surrounding the corpuscle (C), the proximal convoluted tubule, and the distal convoluted tubule are all evident. Group G2 shows swelling of the renal glomerulus and loss of the periglomerular space, with hemorrhage into the renal tissue (H) and enlargement of the distal convoluted tubule and proximal convoluted tubule cells. Group G3, by contrast, displays the renal glomerulus, the surrounding area, and the proximal and distal convoluted tubules in their typical configurations. The renal glomerulus, the area around the glomerulus, and the proximal convoluted tubule and distal convoluted tubule in their typical configuration. They are also visible in group G4.
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References
1. Dhesi M, Maldonado KA, Patel P, Maani CV. Tramadol. In: StatPearls [Internet] [Internet]. StatPearls Publishing; 2024 [cited 2025 Mar 7]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK537060/
2. Rahimi HR, Soltaninejad K, Shadnia S. Acute tramadol poisoning and its clinical and laboratory findings. Journal of research in medical sciences: the official journal of Isfahan University of Medical Sciences. 2014;19(9):855.
3. Lagard C, Chevillard L, Malissin I, Risède P, Callebert J, Labat L, et al. Mechanisms of tramadol-related neurotoxicity in the rat: does diazepam/tramadol combination play a worsening role in overdose? Toxicology and applied pharmacology. 2016;310:108–19.
4. Atici S, Cinel I, Cinel L, Doruk N, Eskandari G, Oral U. Liver and kidney toxicity in chronic use of opioids: An experimental long-term treatment model. J Biosci. 2005 Mar;30(2):245–52.
5. Nakhaee S, Hoyte C, Dart RC, Askari M, Lamarine RJ, Mehrpour O. A review on tramadol toxicity: mechanism of action, clinical presentation, and treatment. Forensic Toxicol. 2021 Jul;39(2):293–310.
6. Shahrajabian MH. Medicinal Herbs with Anti-Inflammatory Activities for Natural and Organic Healing. COC. 2021 Dec;25(23):2885–901.
7. Mueed A, Shibli S, Jahangir M, Jabbar S, Deng Z. A comprehensive review of flaxseed ( Linum usitatissimum L.): health-affecting compounds, mechanism of toxicity, detoxification, anticancer and potential risk. Critical Reviews in Food Science and Nutrition. 2023 Dec 21;63(32):11081–104.
8. Noreen S, Hashmi B, Tufail T, Ikram A, Arshad MT, Gnedeka KT. Synergistic Beneficial Effects of Flaxseed ( LINUM USITATISSIMUM L.) Oil and Olive (Olea europaea L.) Oil Against Metabolic Dysfunction Associated with Fatty Liver and Its Complications. Food Science & Nutrition. 2025 Jan;13(1):e4638.
9. Nowak W, Jeziorek M. The role of flaxseed in improving human health. In: Healthcare [Internet]. MDPI; 2023 [cited 2025 Mar 7]. p. 395. Available from: https://www.mdpi.com/2227-9032/11/3/395
10. Gupta M, Dey S, Marbaniang D, Pal P, Ray S, Mazumder B. Grape seed extract: having potential health benefits. J Food Sci Technol. 2020 Apr;57(4):1205–15.
11. Turcu RP, Panaite TD, Untea AE, Vlaicu PA, Badea IA, Mironeasa S. Effects of grape seed oil supplementation to broilers' diets on growth performance, meat fatty acids, health lipid indices and lipid oxidation parameters. Agriculture. 2021;11(5):404.
12. Yang C, Shang K, Lin C, Wang C, Shi X, Wang H, et al. Processing technologies, phytochemical constituents, and biological activities of grape seed oil (GSO): A review. Trends in Food Science & Technology. 2021;116:1074–83.
13. Ekpono EU, Aja PM, Ibiam UA, Agu PC, Eze ED, Afodun AM, et al. Cucurbita Pepo L. Seed Oil Modulates Dyslipidemia and Neuronal Dysfunction in Tramadol-Induced Toxicity in Wistar Albino Rats. Dose-Response. 2024 Oct;22(4):15593258241290458.
14. Bancroft JD, Gamble M. Theory and practice of histological techniques [Internet]. Elsevier health sciences; 2008 [cited 2024 Aug 16]. Available from: https://books.google.com/books?hl=ar&lr=&id=Dhn2KispfdQC&oi=fnd&pg=PR13&dq=Theory+and+practice+histological+technique&ots=JCkCkuVAD9&sig=wx3VFlp2DL7TuHM4Fph3AdCgSHU
15. Sharma M, Dadhwal K, Gat Y, Kumar V, Panghal A, Prasad R, et al. A review on newer techniques in the extraction of oleaginous flaxseed constituents. OCL. 2019;26:14.
16. Rombaut N, Savoire R, Thomasset B, Bélliard T, Castello J, Van Hecke É, et al. Grape seed oil extraction: Interest of supercritical fluid extraction and gas-assisted mechanical extraction for enhancing polyphenol co-extraction in oil. Comptes Rendus Chimie. 2014;17(3):284–92.
17. Anderoli TE, Carpenter J, Bennett TB. Cecil essentials of medicine : Disorder of lipid metabolism,5th ed. Herbert PN WB Standers company, London, Toronto. 2001;16(2001):526–32.
18. Mohammadnejad L, Soltaninejad K, Seyedabadi M, Ghasem Pouri SK, Shokrzadeh M, Mohammadi H. Evaluation of mitochondrial dysfunction due to oxidative stress in therapeutic, toxic and lethal concentrations of tramadol. Toxicology Research. 2021;10(6):1162–70.
19. Adikwu E, Bokolo B. Prospects of N-acetylcysteine and melatonin as treatments for tramadol-induced renal toxicity in albino rats. Pharmaceutical Sciences. 2017;23(3):172–81.
20. Sobeková A, Piešová E, Maková Z, Szabóová R, Sopková D, Andrejčáková Z, et al. The duration of flaxseed supplementation affects antioxidant defence mechanisms and oxidative stress in fattening pigs. Veterinary Sciences. 2023;10(9):586.
21. Shahid MS, Wu Y, Xiao Z, Raza T, Dong X, Yuan J. Duration of the flaxseed diet promotes deposition of n-3 fatty acids in the meat and skin of Peking ducks. Food & nutrition research. 2019;63:10–29219.
22. Nassiri-Asl M, Hosseinzadeh H. Review of the Pharmacological Effects of Vitis vinifera (Grape) and its Bioactive Constituents: An Update: Pharmacological Effects of Grape. Phytother Res. 2016 Sep;30(9):1392–403.
23. Rodríguez-Martín NM, Montserrat-de la Paz S, Toscano R, Grao-Cruces E, Villanueva A, Pedroche J, et al. Hemp (Cannabis sativa L.) protein hydrolysates promote anti-inflammatory response in primary human monocytes. Biomolecules. 2020;10(5):803.
24. Llobera A. Study on the antioxidant activity of grape stems (Vitis vinifera). A preliminary assessment of crude extracts. Food and Nutrition Sciences. 2012;3(4):500–4.
25. Sayed HY, Zidan AH. Histopathological and biochemical effects of acute and chronic tramadol drug toxicity on liver, kidney, and testicular function in adult male albino rats. J Forensic. 2016;1(2):41.
26. Aprioku JS, Okpe BT, Ben D. Therapeutic levels of short-term tramadol administration negatively affect testis function in rats. Asian Pacific Journal of Reproduction. 2021;10(1):29–35.
27. Singhal PC, Sharma P, Sanwal V, Prasad A, Kapasi A, Ranjan R, et al. Morphine modulates the proliferation of kidney fibroblasts. Kidney International. 1998;53(2):350–7.
28. Piotrowska K, Baranowska-Bosiacka I, Marchlewicz M, Gutowska I, Noceń I, Zawiślak M, et al. Changes in the male reproductive system and mineral metabolism induced by soy isoflavones administered to rats from prenatal life until sexual maturity. Nutrition. 2011;27(3):372–9.
29. Diab KA, Ibrahim NE, Fahmy MA, Hassan EM, Omara EA. Inhibitory activity of flaxseed oil against CdCl2-induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study. Toxicology Reports. 2020;7:1127–37.
30. Farag MF, Osman NN, Darwish MM. Grape Seed Oil as an Anti-hepatic and Anti-renal Dysfunction Agent in Paracetamol-Administered Rats. Journal of Radiation Research and Applied Sciences. 2010;3(3A):707–19.