Study Some Factors Predisposing the Prevalence of Otitis Media in the Diyala Governorate.
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Abstract
Otitis media is a prevalent condition affecting individuals of all ages, often accompanied by symptoms such as ear pain, fluid accumulation, and possible hearing loss. Bacterial infections are among the leading causes, with Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae frequently implicated. This study aimed to identify the bacterial agents responsible for otitis media among patients attending hospitals in Diyala Governorate. A total of 172 ear swab samples were collected between September 1, 2024, and January 1, 2025. Bacterial identification was carried out using biochemical tests and the VITEK 2 system. The findings revealed Pseudomonas aeruginosa as the most prevalent isolate (36%), followed by Staphylococcus aureus and Klebsiella pneumoniae (11% each). Other less frequent isolates included Escherichia coli, Proteus mirabilis, and Staphylococcus epidermidis. Infection was more common among males, individuals aged 16–30, those with lower educational levels, urban residents, and smokers. These results emphasize the necessity of enhanced public health awareness, accurate microbial diagnostics, and responsible antibiotic use to improve treatment outcomes and combat antimicrobial resistance.
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References
1. Al-Jubouri SNA, Dahham SN. Molecular Detection of Virulence Factor Genes for Some Species of Bacteria That Cause Otitis Media in Kirkuk Governorate. Journal of Bioscience and Applied Research. 2024;10(3):452-65.
https://dx.doi.org/10.21608/jbaar.2024.379934
2. Jamal A, Alsabea A, Tarakmeh M. Effect of ear infections on hearing ability: a narrative review on the complications of otitis media. Cureus. 2022;14(7). https://dx.doi.org/10.7759/cureus.27400
3. Algburi A, Abdelhameed A, Naser RH, Alharbi NK, Quinn GA, Popov IV, et al., editors. Antibiotic susceptibility of bacterial isolates from chronic otitis media in Diyala Province, Iraq. AIP Conference Proceedings; 2024: AIP Publishing. https://doi.org/10.1063/5.0191689
4. Abdullah Al-Jubouri SN, Dahham SN. Isolating and Identifying Some Bacterial Types that Cause Otitis Media and Detecting Their Antibiotic Resistance. Kirkuk Journal of Science. 2023;18(4).
https://doi.org/10.32894/kujss.2023.143295.1120
5. Mahmood YS, Abed SM, Alwan AM. Isolation, Identification of Bacterial Species Causing Chronic Suppurative Otitis Media and Detection of Some of Their Virulence Factors. Tik J of Pure Sci. 2019;24(7):45-51.
http://dx.doi.org/10.25130/j.v24i7.910
6. Latif IA. Swarming motility patterns of Pseudomonas aeruginosa isolated from otitis media. Tikrit Journal of Pure Science. 2018;20(4):26-9. https://doi.org/10.25130/tjps.v20i4.1208
7. Forbes BA, Sahm DF, Weissfeld AS. Bailey & Scott's Diagnostic Microbiology. 12th ed. St. Louis, MO: Mosby Elsevier; 2007.
8. Wang L, Hu C, Shao L. The antimicrobial activity of nanoparticles: current status and prospects. International journal of nanomedicine. 2017:1227-49.
http://dx.doi.org/10.2147/IJN.S121956
9. Lamiaa Saud. A. Identify some virulence factors of bacteria, Proteus mirabilis, isolated from people with otitis. Tikrit Journal of Pure Science. 2023;22(8):27-32. https://doi.org/10.25130/tjps.v22i8.848
10. Sah BP, Chettri ST, Bhattarai NR, Shah SP, Paudel D, Sarraf DP, et al. Microbiological profile and antibiotic sensitivity patterns in patients with chronic suppurative otitis media at an eastern tertiary care center in Nepal. IP Journal of Otorhinolaryngology and Allied Science. 2020;3(3):86-90. https://doi.org/10.18231/j.ijoas.2020.019
11. Tadesse B, Shimelis T, Worku M. Bacterial profile and antibacterial susceptibility of otitis media among pediatric patients in Hawassa, Southern Ethiopia: cross-sectional study. BMC pediatrics. 2019;19:1-8.
https://doi.org/10.1186/s12887-019-1781-3
12. Yousif FH, Mohammed SH, Ali MJ. Isolation and identification of bacteria and fungi from patients with otitis media in the city of Hawija and the study of their antibiotic sensitivity. Journal of Education and Scientific Studies. 2024;1(23).
13. AL-Halbousi SMF. The relationship of bacterial otitis media with some environmental factors in AL-Tarmiyah City and its suburbs [Master’s thesis]: Tikrit (Iraq): Tikrit University, College of Science, Department of Biology; 2023.
14. Al-Ani R. Prevalence of otitis media among patients attending the otorhinolaryngology clinic in Ramadi City, Iraq. Egyptian Journal of Ear, Nose, Throat and Allied Sciences. 2020;21(1):17-21. https://dx.doi.org/10.21608/ejentas.2019.15103.1136
15. Mahdiani S, Lasminingrum L, Anugrah D. Management evaluation of patients with chronic suppurative otitis media: A retrospective study. Annals of medicine and surgery. 2021;67:102492. https://doi.org/10.1016/j.amsu.2021.102492
16. Al-Lahibi EFA. Effect of Rosmarinus officinalis L. and Hydrastis Canadensis L. extracts, titanium dioxide and zinc oxide nanoparticles on bacteria isolated from otitis media [Master's thesis]—Tikrit (Iraq): Tikrit University, College of Science, Department of Biology; 2021.
17. Sim JH, Kim KT, Lee SH, Yun SH. Bacteriologic Study of Chronic Suppurative Otitis Media. Korean Journal of Otorhinolaryngology-Head and Neck Surgery. 1997;40(6):819-26.
18. DeAntonio R, Yarzabal J-P, Cruz JP, Schmidt JE, Kleijnen J. Epidemiology of otitis media in children from developing countries: A systematic review. International Journal of Pediatric Otorhinolaryngology. 2016;85:65-74.
https://doi.org/10.1016/j.ijporl.2016.03.032
19. Ahmed AS. Direct identification of bacteria causing otitis media by real-time PCR compared with other methods [Master’s thesis]: University of Mosul, College of Education for Pure Sciences; 2024.
20. Abed IM, Al-Shibly IK, Sultan SSN, Alturaihy SH. Host Immune Responses to Otopathogens during Otitis Media. Annals of the Romanian Society for Cell Biology. 2021;25(6):6040-54.
21. Çetin YS, Mollamehmetoğlu SO, Düzenli U, Turan M, Bozan N. Treatment of multi-drug resistant microorganisms in chronic suppurative otitis media. B-ENT. 2022;18(1):44-51.
https://doi.org/10.5152/B-ENT.2022.21425
22. Gautam N, Shivalingesh K, Kushwaha A, Singh V, Kumar S. Effect of smoking on status of hearing: A cross-sectional study. Archives of Otolaryngology and Rhinology. 2022;8(3):009-13.
https://doi.org/10.17352/2455-1759.000146
23. Paneru M, Shah SP, Chettri ST. Association of passive smoking with otitis media among school children of eastern Nepal. Annals of Otology and Neurotology. 2021;4(02):062-8.