The role of SEM and infrared spectroscopy techniques in identification of the mineralogy of kidney stones: case study from Fallujah City, Western Iraq
Main Article Content
Abstract
The current study is concerned with the mineralogical aspects of kidney stones from urolithiasis patients of different ages and genders in Fallujah city hospital. The following techniques were implemented as follows; X - ray diffraction (XRD) used to recognize the mineral components of the studied kidney stones, Infrared Spectroscopy (IR) to discover the type of chemical compounds of the studied kidney stones, Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDX) to determine the mineralogy and the various chemical components in the kidney stones. The results show that the kidney stones are composed of five mineral groups and these are; mixed stone group, calcium oxalate, phosphate stone, uric acid, and cholesten. The cholesten has been diagnosed for the first time in Iraq, which might be linked to the diet type that includes high levels of cholesterol. Furthermore, the most common mineral group in the study area is the mixed stone which occurs in males more than females for the ages of 40 to 50 years old
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
[1] Lifshitz, D.A.; Shalhav, A.L.; Lingeman, J.E.and Evan, A.P. (1999). Metabolic evaluation of stone disease patients: a practical approach. Journal Endourol, 13(1):669-678.
[2] Stamatelou, K.K.; Francis, M.E.; Jones, C.A.; Nyberg, L.M.and Curhan ,G.C. (2003). Time trends in reported prevalence of kidney stones in the United States: 1976-1994. Kidney International; 63(1): 23- 1817.
[3] Frick, K.K.and Bushinsky D.A. (2003). Molecular mechanisms of primary hypercalcaemia. J Am Soc Nephrol, 1(4):1082-1095.
[4] Bazin, D.; Daudon, M.; Combes, C.and Rey, C. (2012). Characterization and some physicochemical aspects of pathological microcalcifications. Chem. Rev. 11(12): 5092–5120.
[5] Zarasvandi, A.; Carranza, E. J. M.; Heidari, M. and Mousapour, E. (2014). Environmental factors of urinary stones mineralogy, Khouzestan Province, Iran. Journal of African Earth Sciences, 97(1): 368–376. [6] Chandrajith, R. (2019). Mineralogical, compositional and isotope characterization of human kidney stones (urolithiasis) in a Sri Lankan population. Environmental geochemistry and health, 22(1):1-14.
[7] Durgawale, P.; Shariff, A.; Hendre, A.; Patil, S. and Sontakke, A. (2010). Analysis of stones and its significance in urolithiasis. Biomedical Research, 21(3):.1-12.
[8] Evan, A. P. (2010). Physiopathology and etiology of stone formation in the kidney and the urinary tract. Pediatric Nephrology, 25(5): 831– 841.
[9] Holmes, R.P.; Goodman, H.O. and Assimos, D.G. (2001). Contribution of dietary oxalate to urinary oxalate excretion. Kidney International, 59(1):270–276.
[10] Komatina, M.M. (2004). Medical geology: effects of geological environments on human health. Elsevier, Amsterdam: 488pp.
[11] Golovanova, O.; Palchik, N.; Maksimova, N.; and Dar’In, A.V. (2006). Comparative characterization of the microelement composition of kidney stones from patients in the Novosibirsk and Omsk regions. Chemistry for Sustainable Development, 15(1):55-61.
[12] Safarinejad, M. R. (2007). Adult urolithiasis in a population-based study in Iran: prevalence, incidence, and associated risk factors. Urological Research, 35(2):73–82.
[13] Pourmand, G. and Pourmand, B. (2012). Epidemiology of Stone Disease in Iran. In Urolithiasis; Springer London, 12(1): 85–87. [14] Pearle, M.S. and Lotan, Y. (2012). Urinary lithiasis: etiology, epidemiology, and pathogenesis. Campbell-Walsh Urology, 2(1):13-92.
[15] Robertson, W.G. (1969). Measurement of ionized calcium in biological fluids. Clinical Chemistry Acta, 24(1):149–157.
[16] Fullerton, H. (2003). Transposition of directive 2002/46/EC in food supplements: draft food supplements regulations (WALES). PhD Thesis, 5 Bryngelli, Carmel, Llanelli, Carms. 147 P.
[17] Ackermann, D.; Baumann, J.M.; Futterlieb, A. and Zingg, E.J. (1988). Influence of calcium content in mineral water on chemistry and
crystallization conditions in urine of calcium stone formers. European Urology,14(1):305–308.
[18] Kohri, K.et al. (1989). Magnesium-to-calcium ratio in tap water, and its relationship to geological features and the incidence of calcium-containing urinary stones. Journal of Urology, 142(2) :1272–1275.
[19] Bellizzi, V.et al. (1999). Effects of water hardness on urinary risk factors for kidney stones of patients with idiopathic nephrolithiasis. Nephron, 81(1): 66–70.
[20] Nasir S.; Kassem, M.E.; El-Sherif, A.and Fattah, T. (2004).Physical investigation of urinary calculi: example from the Arabian Gulf. State of Qatar, 25(12):1-7.
[21] Bichler, K. H.et al. (2002): Urinary infection stones. International journal of antimicrobial agents, 19(6):488-498.
[22] Al-Naam, L. M.; Baqir, Y.; Rasoul, H.; Susan, L. P. and Alkhaddar, M. (1987). The incidence and composition of urinary stones in southern Iraq. Saudi Medical Journal, 8(5): 456–461.
[23] Al-Maliki, M.A. (1998). Renal stone: A study in medical geochemistry. M.Sc. Thesis, Baghdad Universty Baghdad, Iraq: 100pp (in Arabic).
[24] AL-Shammary, E.J. (2001). Mineralogy and Chemistry of Urinary Stones in Pediatric Age Group in Iraq: A Study in Medical Geochemistry. M.Sc. Thesis. Baghdad University, Baghdad, Iraq:143 p.
[25] Afaj, A.H. and Sultan, M.A. (2005). Mineralogical composition of the urinary stones from different provinces in Iraq. The Scientific World Journal, 5(1):24-38.
[26] Al-Jumaily; Hassan A.A. and Al-Maadhidi; Anwaar S.J. (2018). Chemistry of Major and Trace Elements of Kidney Stones in Fallujah City/Iraq. Journal of Environment and Earth Science, 8(12): pp. 1-7.
[27] Lin, S.M.; Chiang, C.H.; Huang, C.H.;Tseng, C.L.and Yang, M.H. (1985). Instrumental neutron activation analysis of urinary calculi. Journal of Radioanalytical and Nuclear Chemistry, 96(2):153-160. [28] Estepa, L. and Daudon, M. (1997). Contribution of Fourier transform infrared spectroscopy to the identification of urinary stones and kidney crystal deposits. Biospectroscopy, 3 (5): 347-369.
[29] Abboud, I.A. (2008). Mineralogy and chemistry of urinary stones: patients from North Jordan. Environmental Geochemistry and Health, 30(3): 445–463.
[30] Prasanchaimontri, P. and Monga, M.( 2020). Predictive Factors for Kidney Stone Recurrence in Type 2 Diabetes Mellitus. Urology. In Press. Available online 25 April 2020.
https://doi.org/10.1016/j.urology.2020.04.067
[31] Holzbach, R. T.; Marsh, M.; Olszewski, M. and Holan, K. (1973). Cholesterol solubility in bile. Evidence that supersaturated bile is frequent in healthy man. Journal of Clinical Investigations, 52(4):1467–1479. [32] Abu-Farsakh, F. (1997). Correlation between copper, zinc and some lipids in serum, bile and stones of patients with gall stone disease. Dirasat-University of Jordan. Series B: Pure and Applied Sciences, 24(1):54-59.
[33] Hosseini, M.M.; Eshraghian, A.; Dehghanian, I.; Irani, D.and Amini, M. (2010). Metabolic abnormalities in patients with nephrolithiasis: Comparison of first-episode with recurrent cases in Southern Iran. Int. Urol. Nephrol. 42(1): 127–131.
[34] Keshavarzi, B.et al. (2016). Mineralogical composition of urinary stones and their frequency in patients: relationship to gender and age. Minerals,6(4):1-31. [35] Chen, Z., Prosperi, M. and Bird, V. Y. (2019). Prevalence of kidney stones in the USA: The National Health and Nutrition Evaluation Survey’, Journal of Clinical Urology, 12 (4): 296–302. doi: 10.1177/2051415818813820.
[36] Sofia, N.; Manickavasakam, K. and Walter, T. (2016). Prevalence and Risk Factors of Kidney Stone. Global Journal for Research Analysis, 5(3):183-187.
[37] John C. (2014). Lieske: New insights regarding the interrelationship of obesity, diet, physical activity, and kidney stones. Journal of the American society of nephrology, 25(2):211-212.