A bacteriological and histopathological study of sheep pneumonia in Salah Aldeen Governorate

Main Article Content

Noor W. AL-Yousif
Nihad Abdul-Hussain Jafar
Bader Khatlan Hameed

Abstract

This study aimed to isolate and diagnose the bacterial pathogens that causes pneumonia in sheep, study the biochemical and histopathological changes in lung tissue of infected animals and determining the most effective types of antibiotics for treating sheep diseased with pneumonia. 150 samples (50 nasal swabs, 50 tracheal samples, 50 lungs samples from slaughtered sheep) were collected randomly from different areas of Salah Aldeen governorate during the period (September 2019 till January 2020) .


The results showed that no significant differences in the values between positive and negative results from nasal, tracheal and infected lungs at a probability level  P< 0.05. And showed that Staphylococcus aureus and Klebsiella  pneumonia are two main bacterial causes of pneumonia in sheep .


In macroscopic examination the prominent lesion in most lung samples was congestion and presence of emphysema with an expansion of  the bronchitis and  found that the most common type of pneumonia infection is broncho pneumonia.


All the isolated bacterial species; however, show sensitivity to the antibiotics chloramphenicol and trimethoprim + sulfamethoxazole except Pasteurella Spp., that it shows resistance to trimethoprim+ sulfamethoxazole.

Article Details

How to Cite
Noor W. AL-Yousif, Nihad Abdul-Hussain Jafar, & Bader Khatlan Hameed. (2020). A bacteriological and histopathological study of sheep pneumonia in Salah Aldeen Governorate. Tikrit Journal of Pure Science, 25(6), 33–41. https://doi.org/10.25130/tjps.v25i6.309
Section
Articles

References

[1] Stephen O. Akpavie and Tony O. Agyemang. (2019). Histological and immunohistochemical assessments of pneumonia in sheep slaughtered at Ibadan, Nigeria and Kumasi, Ghana. Immunoassay and Immunochemistry J. 40(3): 253-255.

[2] Hind, H.A., Asmaa, H.A. and Mohammed, H. (2016). Isolation And Identification Of Aerobic Bacteria Detected From Sheep Infected With Pneumonia. Adv. Environ. Biol. 10(5): 214-219.

[3] Nahed S. Saleh and Tamer S. Allam. (2019). Pneumonia in Sheep: Bacteriological and Clinicopathological Studies. American Journal of Research Communication, 2 (11): 70-88.

[4] Berhanu Mekibib, Tadesse Mikir, Amene Fekadu, and Rahmeto Abebe.(2019). Prevalence of Pneumonia in Sheep and Goats Slaughtered at Elfora Bishoftu Export Abattoir, Ethiopia: A Pathological Investigation. Veterinary Medicine J. 5 (3):1-10.

[5] Mariane F. Franco2, Natália C. Gaeta, Mario A.R. Alemán, Priscilla A. Mellville, Jorge Timenetsky, Mário F.A. Balaro and Lilian Gregory.(2019). Bacteria isolated from the lower respiratory tract of sheep and their relationship to clinical signs of sheep respiratory disease. Vetenaria Brasiliera. 39(10):796-801.

[6] Hasso S.A.; (2016); An update review of confirmed pathogens of six animal species in Iraq; Iraqi Journal of Veterinary Sciences. 30 ( 1):22-29.

[7] Mohammed A. Y. Alamery and Zainab Shayal Nejiban; (2018); Clinical and diagnostic study of sheep Pneumonic pasteurellosis in Basrah, Iraq . Al-Qadisiyah Journal of Veterinary Medicine Sciences. 17 (1):1-6.

[8] James H. Jorgensen, Karen C. Carroll, Guido Funke. (2020). Identification of Staphylococcus species, Micrococcus species and Rothia species. the National Health Service (NHS) and Public Health Agency(HSC).PP1-26.

[9] Cristina Pesca, Katia Forti , Andrea Felici, Eleonora Scoccia, Claudio Forte , Pietro Antenucci, Sabina Muntoni, Lucia Anzalone , Antonella Dipaolo and Silvia Crotti. (2020). Enzootic pneumonia in sheep: ewe and lamb immune response after Mannheimia haemolytica vaccine administration under field condition in Italy. Animal J. 26: 73-76. [10] Quinn, P.J., B.K. Markey, F.C. Leonard, E.S. Fitz Patric, S. Fanning and P.J. Hartigan. (2011). Veterinary Microbiology & Microbial disease. 2nd ed, UK, WiLey-Blackwell, USA. PP: 67-69 ,745. [11] Bancroft JD and Gamble M (2002): Theory and practice of histological techniques. In: Swisher, B. (ed.), Microorganisms. Churchill Livingstone, Philadelphia. [12] Wayne,et al.. (2011). Performace standards for antimicrobial susceptibility testing; twenty-first informational supplement. Vol. 30 No.1 and Vol. 30 No. 15

[13] Leech, NL, Barrentand G KC, Morgan A. (2007). SPSS for Intermediat Statistics: Use and Interpretation. 1stEdn.,Routledge, London, pp: 20-51.

[14] Zuoyong Zhou, Mengsi Zhang, Hexian Li, Haoyue Yang, Xiaoxia Li1, Xinyue Song and Zhiying Wang.(2017). Prevalence and molecular characterization of Staphylococcus aureus isolated from goats in Chongqing, China. BMC Veterinary Research J. 352(13):2-8.

[15] Katerina Neradova, Vladislav Jakubu, Katarina Pomorska and Helena Zemlickova. (2020). Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic. BMC Veterinary Research. 16:4.

[16] Theophilus A. Jarikre, O. S. Alao and B. O. Emikpe. (2018). Cultural and immunohistochemical evaluation of bacterial agents in sheep pneumonia in Nigeria. Comparative Clinical Pathology. 27(2):1051–1055.

[17] Sonawane, Singh, Tripathi and Kumar Dixit. (2012). Investigation of an outbreak in lambs associated with Escherichia coli O95 Septicaemia; Animal Health Division, Central Sheep and Wool Research Institute, Avikanagar (Rajasthan). j. Veterinary Practitioner. Vol. 13 No. 1

[18] Shahrzad Azizi and Ahmad Oryan .(2013). Pneumonia in slaughtered sheep in south-western Iran: Pathological characteristics and aerobic bacterial aetiology, Veterinaria Italiana, Iran .J.49 (1), 109-118

[19] Dapgh AN, Hakim AS, Abouelhag HA, Abdou AM, Elgabry EA .(2019) .Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat.J. Veterinary World, 12(10): 1524-1528.

[20] Patel, S.S., H.C. Chauhan, A.C. Patel, M.D. Shrimali, K.B. Patel, B.I. Prajapati, J.K. Kala, M.G. Patel, Manish rajgor and Patel, M.A.( 2017). Isolation and Identification of Klebsiella pneumoniae from Sheep-Case Report. Int. J. Curr. Microbiol. App. Sci. 6(5): 331-334. doi:

http://dx.doi.org/10.20546/ijcmas.2017.605.037

[21] Anna Catharina B. Berge, William M. Sischo, Arthur L. Craigmill. )2006(. Antimicrobial susceptibility patterns of respiratory tract pathogens from sheep and goats. Journal of the American Veterinary Medical Association. Vol 229, No. 8.