The Inhibitory Effect of some Plant Extracts on some Pathogenic Bacteria
Main Article Content
Abstract
The purpose of the present study is to assay the inhibitory effect of five concentrations 10, 20, 30, 40 and 50 % of the aqueous and alcoholic extracts for leaves of the Sidr (Zizyphusspin-csit) and Eucalyptus leaves (Eucalyptus camaldulensis) in vitro, against the three types of the experimental bacteria, which Escherichia coli, Staphylococcus aureus and Salmonella typhimurium for their clinical importance as common, pathogenic in urinary tract infection by using the well diffusion assay method .The results showed that antimicrobial activity depends on kind of extract and tested microorganism. and the most bioactivity was done by methanol extract than the aqueous extract, the methanolic extract of Sidr leaves presented the inhibitory effectiveness of all the species used in this study ,the highest inhibition was 36 mm at 50%concentration against E. coli and methanolic extract of eucalyptus leaves showed an inhibitory effect on the growth of all bacteria except E. coli, showed resistance to the extract, As for the aquatic extracts, all the bacteria species illustrated resistance to the aquatic extracts of the leaves of the Sidr except Staph .aureus Inhibition rates were recorded at 17 and 15 mm at the concentration (30 and 20%), respectively, and the aquatic extracts of Eucalyptus leaves had no inhibitory effect on the growth of all the species used in this study, except S. typhimurium bacteria and all concentrations used. The result of the qualitative chemical detection of the active compounds found in the extract indicated that it s contains, mainly like flavonids, alkaloids, Tannins and phenols, Saponins, Glycosides. in aquatic and alcoholic extraction.
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
[1] Joy P. P.; Thomas J.; Mathew S.; Skaria B. P. (1998). Medicinal Plants. Kerala Agricultural University. Aromatic and Medicinal Plants Research Station, India. pp. 1-211.
[2] Nair, R.; Kalariya, T.; Chanda, S. (2005). Antibacterial Activity of Some Selected Indian Medicinal Flora. Turk. J. Biol. Vol. 29: 41-47.
[3] Genetu, A.; Yared, M.; Moges, T.; Andargachew, M. (2008). In vitro antibact-erial activity of crude preparation of myrtle (Myrtus communis) on common human pathogens. Ethiopian medical journal, 46 ( 1):
63-9.
[4] Tepsorn, R. (2009). Antimicrobial Activity of Thai Traditional Medicinal Plants Extract Incorporated Alginate-Tapioca Starch Based Edible Films against Food Related Bacteria Including Foodborne Pathogens. Ph.D. thesis. University of Hohenheim, Thailand, 1-370.
[5] Husein, A. I. A. (2010). Modification of Biologically Active Compounds from Selected Medicinal Plants in Palestine. Thesis for Ph.D. thesis. An-Najah National University, Nablus, Palestine. pp. 1-149.
[6] Nutrition Research (1995) .15 (1): 37-51 .
[7] Wasim, K.; Hag, I. and Asraf, M. (1995). Antimicrobial studies of the leaf of Cannabis sativa L.. Pak. J. Pharm. Sci., 8(1): 29-38.
[8] Al-Rawi, Ali .(1988). Medicinal plants in Iraq. I2, General Authority for Agricultural Research and Water Resources, Ministry of Agriculture and Irrigation.
[9] Schulze, R. (2003). Herbs hands healingltd, california Univ . J ., 8 : 82 -88.
[10] Barnes ,J and Anderson, L.A . (2002). Herbal medicines: A guide for health care professional . 2nd . ed . London pharmaceutical press .
[11] Newall, A.C.; Anderson. A.L. and Phillipson, D.J .(1996). Herbal medicines aguide for health-care professionals. London . The Pharmaceutical Press.
[12] Amin, Ahmed. (2002). Treatment of nasal sinusitis by natural methods -Redine clinic facilities -Road of the wind-treatment -6-infections rayaheen. net. .http; www. rayaheen.net.
[13] Hernandez, M; Lopez, R.; Abanas, R.M.;V. and Arias, A. (1994). Antimicrobial activity of visnea mocanera leaf extracts .J. Ethno pharma cology, 41; 115 -119.
[14] Odebiyi, A., Sofowora, E. A.(1978). Phytochemical Screening of Nigeria Medicinal Plants. Part II, Liyodia. Vol. 403, pp. 234-246.
[15] Saxena, G. ; Faemer, S. ; Hancock, R. E. W. and Towers, G. H. N. (1995) . Antimicrobial compounds from Alnus rubra . J. of pharmcognosy , 33 (1) : 33-36 .
[16] Kuete V, Ngameni B, Simo CCF, Tankeu RK, Ngadjui BT, Meyer JJM, Lall N, Kuiate JR, (2008). Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae). J Ethnopharmacol 120:17-24.
[17] Shihata, I. M. (1951). A pharmacological study of Anagallis arvensis M. D. Vet. Thesis. Cairo Unversity.
[18] Ngbele, J., Yakubu, R. A., Nyam, D. A. (2008). Phytochemical Screening for active Compounds in Canarium schweinfurthii (Atile) leaves from Jos North, Plateau State Nigeria, Research Journal Of Biological Sciences. 11 ( 39): 1076-1078.
[19] Odebiyi, A., Sofowora, E. A.(1978). Phytochemical Screening of Nigeria Medicinal Plants. Part II, Liyodia. Vol. 403, pp. 234-246.
[20] Sofowora, A.( 1982). Medicinal Plants and Traditional Medicine in Africa. John Wiley and sons Ltd. Chichester England, pp. 142-146.
[21] Shami .S.A. (1982). A study of some of Genetic and Toxic traits of Cesium flowering Master thesis , college of Veterinary Medicine-university of Baghdad .
[22] Jaffer, H. J.; Mahmod, M. J.; Jawad, A. M.; Naji, A. and AL-Naib, A.(1983). Phytochemical and biological screening of some Iraqi plan Fitoterapia Lix 299.
[23] Harborne, J. B. (1984). Phytochemical Methods, A guide to modern techniques of plant analysis . 2nd. ed. Chapman and Hail New York .PP.1-4.
[24] Atlas, R. M.; Brown, A. E. & Parks, L. C. (1995). Laboratory Manual of Experimental Microbiology. Mosby Company-Yearbook, Inc., U.S.A.
[25] Abid, Kawther Fouad (2008). Antibacterial activity and anti - Candida in volatile oils of some medicinal plants in Saudi Arabia Microbiology, S.aureus, Bacillus, Escherichia coli in vitro. M.Sc. thesis - College of Education - University of Riyadh.
[26] Al-Rawi, Ali .(1988). Medicinal plants in Iraq. I2, General Authority for Agricultural Research and Water Resources, Ministry of Agriculture and Irrigation.
[27] Kosar, M. Bozan, B. Temelli, F and Baser, K. (2007). Antioxidant activity and phenolic composition of sumac (Rhus coriaria L.) extracts. Food Chem., 103(3): 952-959.
[28] Aytul, K.( 2010). Antimicrobial and Antioxidant Activities OF Olive Leaf Extract and Its Master Of Science ,Izmir p 51- 52.
[29] Fennema, O. R. (1996). "Food Chemistry". 3rd. ed. Marcel Dekker Inc., New York.
[30] Cowan, M. M. (1999). Plant products as antimicrobial agents clin. Microbiol. Rev.; 12(4): 564-582 .
[31] Aliabadi, M. Darsanaki, R, Rokhi, M. Nourbakhsh, M. Raeisi, G. (2012). Antimicrobial activity of olive leaf aqueous extract Scholars Research Library Annals of Biological Research, 3 (8):4189-4191.
[32] Mahasneh, A. M.; Abbas, J. A. and El-Oqilah, A. A. (1996) . Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Bahrain phytotherapy Res. 10 : 257-258 .
[33] Ghazal, S. A.; Auzarqa, M. and Mahasneh, A. M.(1992). Antimicrobial activity of Polygonum equisetiforme extracts and flavonoids. J. Phytotherapy . Res. 6 : 265-269 .
[34] Chakravarty, H. L. (1976). Plant wealth of Iraq, Adictionary of conomic plants volume 1. Botany directorate. Ministry of Agricultuer and agrarian Reform. Baghdad
[35] AL.Ammiri. H.H. (2016). Isolation and Identification of Aerobic Bacteria detection from Sheep infection with pneumonia advances in environmental biology .10 (5): 214-219.
[36] Ahmed, A.A. ; Mahmoud, A.A.; Williams, H.J.; Scott, A.I.; Rribenspies , J.H. and Mabry, T.J. (1993). New sesquiterpene &- methylene lactones from the Egyption plant Jasonia candicans. J. Nat. Prod. 26: 1276-1280.
[37] Takahashi,T. , KOKubo,R. , Sakaino,M.2004. “Anti Microbial activities of Eucalypts leaf extracts and Flavonoids From Eucalyptus maculata”.Letter in Applied microbiology (39) : 60-64.
[38] Trivedi, N.A. and Hotchandani, S.C.(2004). “Astudy of antimicrobial activity of oil of Eucalyptus”. Indian Journal of pharmacology,36 (2) :93-94.
[39] Sule, I.O. and Agbabiaka, T.O. (2008). “Antibacterial Effect of some plant Extracts on selected Enterobacteriaceae”. Department of microbiology, university of Ilovin, P.M.B.1525, Ilorin, Nigeria corresponding E-mail. Is Email ao lawales@yahoo.co.uk. Issued (17) November (2008).
[40] Al-Rubayee, Zaid Shaker Naji. (2000). Effect of aquatic and alcohol extracts of Citrullus colocynthis and Solanum nigrum in the growth of some isolated bacteria from burns. M.Sc. thesis. College of Science. Mustansiriya University.
[41] Saleh, D. S.(1990). Microbiology. Committee of teaching the Department of Life Sciences. Dar al-Hikma . Baghdad University . Iraq.
[42] Hanafy , M. S. and M. E. Hatem (1991). Studies on the antimicrobial activity of Nigella sativa seeds (black cumin ). J, Ethnopharmacol.34 (2-3):275-278.
[43] Korukluoglu M, Sahan Y, Yigit A, Ozer ET, Gucer S.(2010). Antibacterial activity and chemical constitutions of Olea europaea L. leaf extracts. Journal of Food Processing and Preservation; 34: 383-396.