Physiological and histological study of the effect of Cumin Cyminum watery extract and vitamin E on the male reproductive system in rats exposed to oxidative stress
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Abstract
This study was conducted to investigate the effect of watery extract of Cumin plant (200 mg/kg B.W.) on some physiological and histological parameters of the male reproductive system in white albino rats exposed to oxidative stress by Hydrogen peroxide (H2O2 0.5%). Thirty adult male rats weighting about (200-250 g) and 10-12 weeks age were used in the present study and divided into (5) groups, 6 rats per group, group1: serve as a control group and given normal diet with drinking water, group2: Administrated H2O2 0.5% with drinking water, group3: Given Cumin extract (200 mg/kg B.W.) through oral tubular feeding + H2O2 (0.5%), group4: Given Cumin extract (200 mg/kg B.W.) alone and group5: Administrated Vitamin E 500 (mg/kg B.W.) with H2O2 0.5%, the experiment were continued 30 days for all groups. The results of the current study showed that treatment with H2O2 resulted significant increase (P<0.05) in malondialdehyde, significant decrease (P <0.05) in glutathione and significant decrease (P<0.05) in serum testosterone compared with control group, While the treatment with H2O2 plus the water extract of Cumin seeds (200 mg/kg B.W.) showed a significant decrease (P<0.05) in malondialdehyde, a significant increase in glutathione and a significant increase in testosterone in the serum compared to the H2O2 group. As for ectopic tissue, H2O2 has resulted in satisfactory tissue changes, while water extract (200 mg/kg) with H2O2 resulted in little improvement of the testes tissue. The water extract of Cumin plant acts as an antioxidant through its important role in curbing the harmful effects of some types of free radicals within the body and thus repair the damage in most of the physiological and histological changes and functions of the male reproductive system of rats with exposure to oxidative. Histological section of the testes in the rats treated with vitamin E and H2O2 observed repair of the cells lining the seminiferous tubules with the continuation of the formation of sperm, on the other hand the results showed that giving the Cumin for a long periods lead to reduce the level of hormone testosterone
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