Prophylactic role of lactic acid prepared from lactobacillus acidophilus on cryptosporidiosis
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Abstract
Cryptosporidium has considered as a prominent enteropathogen of humans and animals. Until recently, there is no totally effective therapy other than a healthy intact immune system. Probiotics reported to stimulate both innate and acquired immunity at mucosal and systemic levels. It has been suggested that probiotics inhibit infection by excretion of substances harmful to one of the parasite’s developmental stages and possibly offer new therapeutic factors for the treatment of cryptosporidiosis .The aim of the present study was to detect the prophylactic effect of lactic acid prepared by Lactobacilli acidophillus against the infection with Cryptosporidium in an rabbits model, by estimating hematological and immunological criteria. The results showed that the using of probiotics induced significant reduction in parasite burden, lymphocyte numbers were increased significantly in infected and treated animals. There is a significant increase in the level of IgG ,IgM , C3 and C4 in infected animals.
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References
[1] Checkley, W.; White, A.C.; Jaganath, D.; Arrowood, M.J.; Chalmers, R.M. and Chen, X, (2015). A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium. Lancet Infect Dis;15: 85–94.
[2] Shirley, D.A.; Moonah, S.N.; Kotloff, K.L. (2012). Burden of disease from cryptosporidiosis. Curr Opin Infect Dis: 25(5):555–563.
[3] Nemes, Z.(2009). Diarrhea from the infectologist’s point of view. Orv Hetil : 150(8):353–361
[4] Speich, B.; Croll, D.; Furst, T. ; Utzinger, J. and Keiser, J.(2016) Effect of sanitation and water treatment on intestinal protozoa infection: a systematic review and meta-analysis. Lancet Infect Dis; 16:87–99.
[5] Ehsan, A.M.; Geurden, T.; Casaert, S.; Parvin, S.M.; Islam, T.M.; Ahmed, U.M.;Levecke, B.; Vercruysse, J. and Claerebout, E. (2015). Assessment of zoonotic transmission of Giardia and Cryptosporidium between cattle and humans in rural villagesin Bangladesh. PLoS One, 10(2), 1–11.
[6] Yang, Y.; Zhou, y. Xiao, P. and Shi, Y.(2017). Prevalence of and risk factors associated with Cryptosporidium infection in an underdeveloped rural community of southwest China, Yang et al. Infectious Diseases of Poverty, DOI 10.1186/s40249-016-0223-9.
[7] Bowman, D.D., Lynn, R.C. and Eberhard, M.L. (2003). Georgis parasitology for veterinarians. 8th. ed. Saunders, St. Louis, pp: 98-100.
[8] Maria, J.; Saez, L.; Carolina G.; Julio P. and Angel, G. (2015). "The Role of Probiotic lactic acid bacteria and Bifidobacteria in the Prevention and Treatment of Inflammatory Bowel Disease and Other Related Diseases: A Systematic Review of Randomized Human Clinical Trials". Biomed Res Int (Systematic review). : 15. doi:10.1155/2015/505878. PMC 4352483PMID 25793197 [9] Singhi, S.C. and Kumar, S. (2016). "Probiotics in critically ill children.". F1000 Res (Review). 5:407. doi:10.12688/f1000research.7630.1. PMC 4813632. PMID 27081478
[10] Henriksen, S.A. and Pohlenz, J.F.J. (1981). Staining of Cryptosporidia by a modified Ziehl-Neelsen Technique. Acta Vet. Scand. 22: 594-596.
[11] Anderson, B.C. (1981). Petterns of shedding of cryptosporidial oocyst in Indah calves. J. Am. Vet .Med. Assoc:178 (9):982-984.
[12] Arrowood, M.J. and sterling,C.R.(1987). Isolation of Cryptosporidium oocyst and Sporozoite using discontinuous sucrose and isopoycnic percoll gradients .J.parasitol.,73:314-319.
[13] Baron, E.J.; Peterson, L.R. and Finegold, S. M. (1994): Bailey and Scotts diagnostic Microbiology. 8th ed. Mosby Year book. Inc. America. 792.
[14] Savage, D.C. (1984). Adherence of the normal flora in: attachment of organism to the gut mucosa. voll. Boedeker, E.C.; Bocaraton crc press.pp. 3-10.
[15] Fuller, R. (1975). Nature of the determenat responsible for adhesion of Lactobacillus to chicken crop epithelial cell. J .microbial.: 87(2):245-150.
[16] Henry L. and Davidson, I. ( 1974). Clinical diagnosis by lab. Methods, Saunders Company. Landon.
[17] Laxer, M.A.; Alcantara, A. K.; Javato-Laxer, M.; Menorca, D.M.; Fernado, M.T. and Ronga, C.P. (1990). Immune response to cryptosporidiosis in philippine children. Am. J. Trop. Med. Hyg.: 42: 131-139.
[18] Nawaf, M.G. (2005). The use of Lactobacillus acidophilus as a probiotic for the prevention and treatment of experimental rat enteritis.Msc.th. College of medicine, Tikrit University.
[19] Muhsen, R.K. (2007). The use of Lactobacillus acidophilus as a probiotic in the prevention and treatment of Salmonella typhimurium infection in puppies' .PH.D. Thesis. College of Veterinary Medicine. University of Baghdad
[20] Alak, J. I.; Wolf, B.W.; Mdurvwa, E.G.; Pimentel-Smith, G.E.; Kolavala, S.; Abdelrahman, H.and Suppiramaniam,V. (1999). Supplementation with Lactobacillus reuteri or L. acidophilus reduced intestinal shedding of Cryptosporidium parvum oocysts in immunodeficient C57BL/6 mice. Cell Mol Biol. :45(6):855-863.
[21] Jens, W.r (2015). Ecological Role of Lactobacilli in the Gastrointestinal Tract: Implications for Fundamental and Biomedical Research. Applied and Enviromental Microbiology: l(81), Issue(23).
[22] Schiffrin, E.J., Rochat, F., Link-Amster,H., Aeschlimann, J. M., and Donnet-H. (1995). Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. J. Dairy Sci.78: 491-497.
[23] Isolauri E.; Sütas Y.; Kankaanpää, P.; Arvilommi, H. and Salminen. S. (2001). Probiotics: effects on immunity. Am J Clin Nutr.73 (2):444S-450S.
[24] Haghighi, H.R.; Gong, J.; Gyles, C.L.; Hayes, M.A.; Sanei, B. Parvizi, P.; Gisavi, H.; Chambers, J.R. and Sharif. S. (2005). Modulation of antibody-mediated immune response by probiotics in chickens. Clin. Diagn. Lab. Immunol. 12: 1387-1392.
[25] Riggs, M. W. (2002). Recent advances in cryptosporidiosis: The immune response. Microbes and Infection. 4(10), 1067-1080
[26] McDonald V.; Korbel, D.S.; Barakat F.M.; Choudhry N. and Petry F. (2013). Innate immune responses against Cryptosporidium parvum infection. Parasite Immunol., 35 (2): 55–64.
[27] Gupta V. and R. Garg (2009). Probiotics. Ind J Med Microbiol. 27(3):202–209
[28] Wollowski, I.; Rechkemmer, G. and B. L. Pool-Zobel. (2001). Protective role of probiotics and prebiotics in colon cancer. Am. J. Clin. Nutr. 73: 451S - 455S