Detection of auto-transporter Sat and Vat genes among Escherichia coli strains isolated from urinary tract infections
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Abstract
This study was designed to detect the presence of genes encoding autotranspoter proteins in E. coli that causes UTI by using PCR techniques. Seventy two urine sample were collected from patients infected with UTI whom attended to Salah-AL-deen general hospital in Tikrit city, during three months period (September to November 2016). All samples were cultivated on Blood agar and MacConkey agar. The 47(65.2%) E. coli isolates were confirmed using standard biochemical tests for E. coli. The results indicate the frequencies of Sat gene was 27 strains(57.5%) while Vat gene was 12 strains (25.5%) while the Duplex PCR detected 8(17%) strains of E. coli contained two genes. With this method, we confirmed that autotransporter genes are pathospecifically distributed among the E. coli strains studied.
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References
[1] Santo, E.; Salvador, M.M. and Marin, J.M. (2007). Multidrug-Resistant Urinary Tract Isolates of Escherichia coli from Ribeira Oreto, Sao Paulo. Braz. J. Infect. Dis. 11(6):1-5
[2] Kaper, J.B.; Nataro, J. P. and Mobley, H. L. (2004). Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2:123–140.
[3] Russo, T.A. and Johnson, J.R. (2003). Medical and economic impact of extraintestinal infections due to Escherichia coli: focus on an increasingly important endemic problem. Microbes Infect., 5:449–456.
[4] Russo, T.A. and Johnson, J.R. (2000). Proposal for a new inclusive design nation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC. J. Infect. Dis.,181:1753–1754.
[5] Marrs, C.F.; Zhang, L. and Foxman, B.(2005). Escherichia coli mediated urinary tract infections: are there distinct uropathogenic E. coli (UPEC) pathotypes? FEMS Microbiol. Lett., 252:183–190.
[6] Guyer, D.M.; Radulovic, S., Jones, F.E., et al.(2002). Sat, the secreted autotransporter toxin of uropathogenic Escherichia coli, is a vacuolating cytotoxin for bladder and kidney epithelial cells. Infect. Immun.; 70 (8): 4539-46.
[7] Henderson, I.R.; Navarro-Garcia, F., Desvaux, M. et al. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev., 68 (4): 692-744.
[8] Dutta, P.R.; Cappello, R., Navarro-Garcı´a, F. and Nataro, J.P. (2002). Functional comparison of serine protease autotransporters of Enterobacteriaceae. Infect. Immun.,70:7105–7113.
[9] Parham, N.J.; Srinivasan, U., Desvaux, M., Foxman, B., Marrs, C. F. and Henderson, I.R. (2004). PicU, a second serine protease autotransporter of uropathogenic Escherichia coli. FEMS Microbiol. Lett., 230:73–83.
[10] Roche, A.; McFadden, J. and Owen, P.(2001). Antigen 43, the major phase-variable protein of the Escherichia coli outer membrane, can exist as a family of proteins encoded by multiple alleles. Microbiology, 147:161–169.
[11] Parreira, V.R.; and Gyles, C.L. (2003). A novel pathogenicity island integrated adjacent to the thrW tRNA gene of avian pathogenic Escherichia coli encodes a vacuolating autotransporter toxin. Infect. Immun.,71:5087–5096.
[12] Forbes, BA.; Sahm, D.F. and Weissfled, A.S. (2007). Baily & Scott’s Diagnostic Microbiology. 12th ed. St. Louis Missouri: Mosby.
[13] Saraylu, J; Fallah, M. J; Imani F. AA; Sabaghi, A; Molla, A. H; Hasankhani M. (2012). Prevalence and Evaluation of Toxin Genes among Uropathogenic
Escherichia coli Clinical Isolates by Duplex PCR. J. Med. Bacteriol., 1 (1, 2): pp. 17-22.
[14] Ejernaes K. (2011). Bacterial characteristics of importance for recurrent urinary tract infections caused by Escherichia coli. Dan Med Bull; 58 (4): B4187.
[15] Abdallah K.S., Cao Y., Wei D.J. (2011). Epidemiologic Investigation of Extra-intestinal pathogenic E. coli (ExPEC) based on PCR phylogenetic group and fimH single nucleotide polymorphisms (SNPs) in China. Int J Mol Epidemiol Genet; 2 (4): 339-53.
[16] Hagan E.C., Mobley H.L.T. (2007) Uropathogenic Escherichia coli Outer Membrane Antigens Expressed during Urinary Tract Infection. Infect Immun; 75 (8): 3941-9.
[17] Sharma, A.; Shrestha, S., Upadhyay, S., and Rijal, P. (2011). Clinical and Bacteriological profile of urinary tract infection in children at Nepal Medical College Teaching Hospital Nepal Med Coll J; 13(1): 24-26.
[18] Khoshbakht R, Salimi A, Shirzad Aski H, Keshavarzi H. (2013). Antibiotic Susceptibility of Bacterial Strains Isolated From Urinary Tract Infections in Karaj, Iran. Jundishapur J Microbiol.;6(1):86-90. DOI: 10.5812/jjm.4830.
[19] Mahesh, E.; Ramesh, D., Indumathi, V.A., Punith, K., Raj, K., and Anupama, H.A. (2010). Complicated urinary tract infection in a tertiary care centre in south India, Al amen journal of medical sciences;3(2):120-127.
[20] Paryani, J.P.; Memon, S.R., Rajpar, Z.H., Shah, S. A. (2012). Pattern and Sensitivity of Microorganisms Causing Urinary Tract Infection at Teaching Hospital JLUMHS ; Vol 11: No. 02.,97-100.
[21] Koch, V.H.; Zuccolotto, S.M.C. Urinary tract infection: a search for evidence. J Pediatr (Rio J)2003;79 Suppl 1:S97-S106.
[22] Smith, D.R. (1993). Agarose gel electrophoresis. Methods in molecular biology: Transgenesis Techniques. (D Murphy & DA Carter, Ed.). Humana Press Inc., Totowa, NJ.
[23] Guyer, D.M.; Henderson, I.R., Nataro, J.P. and Mobley, H.T.L. (2000). Identification of Sat, an autotransporter toxin produced by uropathogenic Escherichia coli. Mol. Microbiol., 38: 53–66. 21-
[24] Maroncle, N.M.; Sivick, K.E., Brady, R. et al., (2006). Protease activity, secretion, cell entry, cytotoxicity, and cellular targets of secreted autotransporter toxin of uropathogenic Escherichia coli . Infect. Immun., 74 (11): 6124-6134.
[25] Nichols, K.B.; Totsika, M., Moriel, D.G., Lo, A.W., Yang, J., Wurpel Daniël J., Rossiter Amanda E., Strugnell Richard A., Henderson Ian R., Ulett Glen C., Beatson, S. A. and Schembri, M.A.(2016). Molecular Characterization of the Vacuolating Autotransporter Toxin in Uropathogenic Escherichia coli. jb.asm.org. Vol.198:No.(10):1487-1498.
[26] Ewers, C.; Li, G., Wilking, H., Kiebling, S., Alt, K., Antao, E. M., Laturnus, C., Diehl, I., Glodde, S., Homeier, T., Bohnke, U., Steinruck, H., Philipp, H.C. & Wieler, L.H. (2007). Avian pathogenic, uropathogenic and newborn meningitis-causing Escherichia coli : J How closely related are they? Int J Med Microbial;297:163-176.
[27] Heimer, S.R.; Rasko, D.A., Lockatell, C.V., Johnson, D.E. and Mobley, H.L. (2004). Autotransporter genes pic and tsh are associated with Escherichia coli strains that cause acute pyelonephritis and are expressed during urinary tract infection. Infect. Immun., 72:593–597.
[28] Vila, J.; Vargas, M., Henderson, I.R., Gascon, J.and Nataro, J.P.(2000). Enteroaggregative Escherichia coli virulence factors in traveler’s diarrhea strains. J. Infect. Dis.,182:1780–1783.
[29 Escobar-Paramo, P.; Clermont, O., Blanc-Potard, A.B., Bui, H., Le Bouguenec, C. and Denamur, E. (2004). A specific genetic background is required for acquisition and expression of virulence factors in Escherichia coli. Mol. Biol. Evol., 21:1085–1094.