The prevalence of the Cryptosporidium parvum among children in Ramadi city
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Abstract
The purpose of this study was to determine the prevalence of Cryptosporidium parvum among children in the city of Ramadi and made a comparison between ziehl -Neelsen method and the ELISA method, which is usually used to diagnose this parasite in Ramadi hospital (administration of Obstetrics and Gynecology Hospital and Ramadi Teaching Hospital).
Fecal samples were collected from 813 children under 5 years. Oocysts of Cryptosporidium parvum parasite were determined by using ELSIA and Ziehl-Neelsen (acid-fast stain, ZN).
Our study was shown that the rate of prevalence of the C. parvum was 7.87% from the total of 813 samples of diarrhea by using Zeihl-Neelsen method while ELISA technique result was 11.56% From our results, ELISA technique more specific than Ziehl-Neelsen method in diagnosis of C. parvum and the different is significant (P <0.05)..
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References
[1] Zintl, A., Proctor, A. F., Read, C., Dewaal, T., Shanaghy, N., Fanning, S., & Mulcahy, G. (2009). The prevalence of Cryptosporidium species and subtypes in human faecal samples in Ireland. Epidemiology & Infection, 137(2), 270-277.
[2] Troeger, C., Forouzanfar, M., Rao, P. C., Khalil, I., Brown, A., Reiner Jr, R. C., ... & Mokdad, A. H. (2017). Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015. The Lancet Infectious Diseases, 17(9), 909-948.
[3] Leitch, G. J., & He, Q. (2011). Cryptosporidiosis-an overview. Journal of biomedical research, 25(1), 1-16.
[4] Pignata, C., Bonetta, S., Bonetta, S., Cacciò, S. M., Sannella, A. R., Gilli, G., & Carraro, E. (2019). Cryptosporidium Oocyst contamination in drinking water: a case study in Italy. International Journal of Environmental Research and Public Health, 16(11), 2055.
[5] Checkley, W., White Jr, A. C., Jaganath, D., Arrowood, M. J., Chalmers, R. M., Chen, X. M., ... & Houpt, E. R. (2015). A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium. The Lancet Infectious Diseases, 15(1), 85-94.
[6] Alali, F., Abbas, I., Jawad, M., & Hijjawi, N. (2021). Cryptosporidium infection in humans and animals from Iraq: a review. Acta Tropica, 220, 105946 .
[7] Marques, F. R., Cardoso, L. V., Cavasini, C. E., Almeida, M. C. D., Bassi, N. A., Almeida, M. T. G. D., ... & Machado, R. L. D. (2005). Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples. Brazilian Journal of Infectious Diseases, 9(1), 3-5.
[8] AL-Ezzy, A. I. A., Khadim, A. T., & Humadi, A. A. (2021, June). Clinical Agreements Between Ziehl Neelsen And Methylene Blue Staining Modifications For Detection Of C. parvum Infection In Claves. In Proceedings of 2nd National & 1st International Scientific Conference (Vol. 1, No. 2).
[9] Nichols, R. A., Campbell, B. M., & Smith, H. V. (2006). Molecular fingerprinting of Cryptosporidium oocysts isolated during water monitoring. Applied and environmental microbiology, 72(8), 5428-5435.
[10] Farhang, H. H. (2017). Isolation of Cryptosporidium parvum Oocyst From Infected Feces. Crescent Journal of Medical and Biological Sciences, 4(3), 150-152.
[11] Al-Zubaidi, M. T. S. (2009). Some epidemiological aspects of Cryptosporidiosis in goats and Ultrastructural study (Doctoral dissertation, Doctoral thesis submitted to the university of Baghdad-college of veterinary medicine).
[12] Kawan, M. H. (2018). Calculation of the Shedding Rate of Cryptosporidium Oocysts from the Natural Infected Sheep. Iraqi Journal of Agricultural Sciences, 49(3).
[13] Qader, A. M. A., Kubti, Y., & Khan, A. H. A Comparative Evaluation of Stool Microscopy and Coproantigen - ELISA in the Diagnosis of Cryptosporidiosis.
[14] Carisbad EIA3467 crypto. Ag stool DRG (CA.2016). [15] Khanal A.B Biostatistics for Medical Students and Research Workers , Jaypee Brothers Medical Publishers, New Delhi, Indi a 2016.
[16] Peat, J., & Barton, B. (2008). Medical statistics: A guide to data analysis and critical appraisal. John Wiley & Sons.
[17] Khurana, S., & Chaudhary, P. (2018). Laboratory diagnosis of cryptosporidiosis. Tropical parasitology, 8(1), 2.
[18] Medema, G. J., Schets, F. M., Teunis, P. F. M., & Havelaar, A. H. (1998). Sedimentation of free and attached Cryptosporidium oocysts and Giardia cysts in water. Applied and Environmental Microbiology, 64(11), 4460-4466.
[19] Borowski, H., Thompson, R. C. A., Armstrong, T., & Clode, P. L. (2010). Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system. Parasitology, 137(1), 13-26.
[20] Arrowood, M. J. (2002). In vitro cultivation of Cryptosporidium species. Clinical Microbiology Reviews, 15(3), 390-400.
[21] Chappell, C. L., Okhuysen, P. C., Sterling, C. R., & DuPont, H. L. (1996). Cryptosporidium parvum: intensity of infection and oocyst excretion patterns in healthy volunteers. The Journal of Infectious Diseases, 173(1), 232-236.
[22] Rahi, A. A., Magda, A., & Al-Charrakh, A. H. (2013). Prevalence of Cryptosporidium parvum among children in Iraq. American Journal of Life Sciences, 1(6), 256-260.
[23] Checkley, W., Epstein, L. D., Gilman, R. H., Black, R. E., Cabrera, L., & Sterling, C. R. (1998). Effects of Cryptosporidium parvum infection in Peruvian children: growth faltering and subsequent catch-up growth. American journal of epidemiology, 148(5), 497-506.