Antibacterial extract and active content activity for Erodium cicutarium
Main Article Content
Abstract
Result revealed that all tested extract( hot and cold water) alcoholic (ethanol & methanol) glycosidic and tannic extract at concentration 25, 50, 100 mg/ml were tested of Erodium cicutarium species (Geraniaceae) grown in north and middle of Iraq for their antibacterial (against some gram negative Staphylococcus aureus , Streptococcus lactis & Gram negative Eschichia coli , Proteus mirabilis pathogenic bacteria) That the methanol extract and extract glycosid an average of 14.540 and 14.530 mm, respectively, are the most effective against the bacteria studied compared to other tested extracts. The results also show that there is variation in the concentration of the active compounds studied depending on the environment (geographical location) Tannin and flavonoid content of aerial plant part and different plant part (aerial part, flower, fruit, root) was assessed for studied plant using HPLC techniques, and depending on the plant part to the rutin showed the highest concentration in the vegetative parts compared to other compounds and vegetable segment studied.
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
1- Miller. D.M. (2002). The taxonomy of Pelargonium species and cultivars, their origins and growth in the wild. Geraniums and Pelargonium. The genera Geranium and Pelargonium. In Maria Lis-Balchin (ed.). Medicinal and Aromatic Plants-Industrial Profiles. Published by Taylor and Francis, London. pp. 49-79.
2- Iancu. C.E, Cioancă. O. Hăncianu. M and Mircea. C. (2016). Phytochemical profile OF Two cultivated pelargonium (Geraniaceae) Species. FARMACIA, 64, (6): 840-843.
3- Wagh. V.V. Datt. B. Husain. T. (2015). An Assessment of Diversity of Genus Geranium L. (Geraniaceae) in India withSpecial Emphasis on Indian Himalayan Region. Journal of Biodiversity Management and Forestry 4(2) :1-6.
5- Meran . S.H.S.(2009) . Systematic study of the genera Biebersteinia Stephan and Geranium L. (Geraniaceae) in Iraqi Kurdistan Region . Doctoral thesis , University of Sulaimany .Iraq.
6- Ben Hsouna A., Hamdi N.(2012).Phytochemical composition and antimicrobial activities of the essential oils and organic extracts from Pellargonium graveolens growing in Tunisia. Lipids in Health and Disease, 11:167.
7- Leyton, S,M; Beltramino. J.B.(2015). Evaluation of a Nipple Sealer Based on Brad Infusion. "Science Stays True Here .Biological and Chemical Research, Volume, 345-355 | Science Signpost Publishing
8- Igwenyi, I.O.& Elekwa, A.E.(2016). hytochemical Analysis and Determination of Vitamin Contents of Geranium Robertianum. IOSR Journal of Dental and Medical Sciences (IOSR-JDMS). e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 13, Issue 6 Ver. , PP 44-47.
9- Bautista. M; Madrigal –Santillán. E; Morales -González.A; Gayosso–del ucio.J.A; Madrigal -Bujaidar. E; Chamorro -Cevallos. G; Álvarez -G onzález.I; Benedi. J; Aguilar-Faisal. J.L; Morales -González. J.A. (2015). An Alternative hepatoprotective and Antioxidant agent: The Geranium . Afr J Tradit Complement Altern Med. . 12(4): 96-105.
10- Lakhdari W, Dehliz A, Acheuk F, Mlik R, Hammi H, Doumandji-Mitiche. B, Gheriani. S, Berrekbia. M, Guermit. K, Chergui. S.(2016). Ethnobotanical study of some plants used in( Algerian Sahara)raditional medicine in the region of Oued Right. Journal of Medicinal Plants Studies; 4(2): 204-211
11- Petrova. I; Petkova. N; Ivanov.I.(2016). Five Edible Flowers – Valuable Source of Antioxidants in Human Nutrition. International Journal of Pharmacognosy and Phytochemical Research; 8(4); 604-610.ISSN: 0975-4873
12- Grand, A ., Verpoort , R., Wondergem, P.A . and Pousset , J.L.(1988). Anti-infection phytotherapies of tree-Sarannah Sengal (west-africa). 11-Antimicrobial activity of 33 species . J. Ethanopharmacol., 22:25-31.
13- Ukida, M.; Akihisa, T.; Yasukawa, K.; Tokuda, H.; Suzuki, T. & Kimura, Y. (2006) .Anti- in flammatory, anti- tumor promoting and cytotoxic activities of constituents of Marigold (Calendul aofficinallis) flowers. J. Nat. Prod., 96:1692- 1696.
Ahmad, M. & Nazil, S. (1989). Studies on tannins from bark of pinus roxburghi. J . Chem. Soc. Pakis., 15: 213- 217.
14- Mitscher, L.A, Leu, R., Bathala, M.S. ,Beal, J.L. and White, R.(1972). Antimicrobial agents from higher plants . lioydia ; 35:157-166 .
15- Seal .T.(2016). Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India. Journal of Applied Pharmaceutical Science , 6 (02: 157-166.
16- Egorove, N.S. (1985). Antibiotics a scientific approach . Mir Publishers .Moscow.
17- Öhretoulu. D. Sakar. M. K. Sterner. O. (2011). Polyphenolic compounds from Geranium purpureum Vill. growing in Turkey . Turk J Chem., 35: 809 – 814.
18- Fecka. I. Kowalczyk. A. Cisowski. W. (2001). Phenolic Acids and Depsides from Some Species of the Erodium Genera. Z. Naturforsch. 56c, 943-950
19- Pantev. A., Ivancheva. S., Staneva. L And Serkedjieva. J. (2006). Biologically Active Constituents of a Polyphenol Extract from Geranium sanguineum L. with Anti-Influenza Activity . Verlag der Zeitschrift für Naturforschung, Tübingen . Naturforsch. 61c, 508-516
20- Gohar. A.A. Lahloub. M.F. Niwa. M. (2003). Antibacterial Polyphenol from Erodium glaucophyllum. Verlag der Zeitschrift für Naturforschung, Tübingen. 58c,pp: 670-674.
21- Radulovic . N , Dekic . M , Stojanovic Radi´ C. Z .(2011). Chemical composition and antimicrobial activity of the essential oils of Geranium columbinum L. and G.lucidum L. (Geraniaceae). Turk J Chem. Tubitak. 499 – 512
22- Nostro, N., Germano, M. P., dangeto, V., Marino, A. and Cannatelli, M. A. (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letter in Applied Microbiology, 30 : 379 – 384.
23- Fazary, A. E., Taha, M., & Ju, Y. H. (2008). Iron complexation studies of gallic acid. Journal of Chemical & Engineering Data, 54(1), 35-42
24- Cowan, M. M. (1999). Plant products as antimicrobial agents. Clin. Microbiol. Rev.,12: 564- 582.
26- Okuda, T. 2005. Systematics and health effects of chemically distinct tannins in medicinal plants. Phytochem., 66: 2012- 2031.
27- Engels, C.; Schieber, A. & Ganzle, M. G.(2011). Inhibitory spectra and modes of antimicrobial action
of gallotannins from mango kernels (Mangifera india L.). Appl. Environ. Microbiol., 77: 2215- 2223.
28- Dyubeni, L.; Mayekiso, B. and Magwa, M.L. (2012). A comparative study on essential oil yield and composition of rose-scented geranium (P. c. v. Rose) commercially grown on three different sites of the Amathole region in the eastern Cape, South Africa. African Journal of Agricultural Research, 7(43): 5842- 5848.
29- Abd El-Wahab, M.A.; Toaima, W.I.M. and Hamed, E.S. (2016). Effect of different planting locations in Egypt on volatile oil of geranium (Pelargonium graveolens L.) plant. Journal of basic and applied Research, 2(4): 522-533