Isolation of Trypsin from Serum of pancreatic Cancer Patients and determination some biochemical parameters
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Abstract
The research included isolation and purification of Trypsin from serum of patient with pancreatic cancer, and estimation its molecular weight by using the two techniques of gel filtration (using sephadex G-50) and electrophoresis (using SDS). Therefore; it was noted that the proteinous peak isolated from ammonium sulfate solution is saturated (65%) of the serum by using the two techniques gel filtration and electrophoresis had apparent molecular weight (27000) Dalton. The study included (40) patients with pancreas cancer their ages ranged between (30-70) years who attended Al-Jumhuri and Atomic Medicine Hospitals in Mosul; the laboratory examination included estimation levels of: Trypsin, Gamma Glutamyl transferase, Amylase, Paraoxonase ,Glutathione, Sialic acid and Ceruloplasmin. Results showed that level of Trypsin and Amylase in the serum decreased significantly in patients with pancreatic cancer in comparison with the control group, but levels of Gamma Glutamyl transferase, paraoxonase, Glutathione, Sialic acid and Ceruloplasmin increased significantly in patients with pancreatic cancer in comparison with the control group.
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