Molecular detection of Hemolycin in Escherichia coli and attempt to inhibition by using the Probiotics
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Abstract
The current study included of (60) urine samples were collected from patients suffering from urinary tract infection in Tikrit Hospital during the period from April 2017 to June 2017 The results of identification showed that among (49) at percentage (81.6 %) positive samples were found (24) of samples with percentage (48.97 %) were Escherichia coli isolates .
The susceptibility of isolates to avariety of antibiotics has been investigated, the results revealed that the isolates were (100%) resistant to (Erythromycin and Nalidixic acid), also these isolates have the highest percentage of resistant to Ceftriaxone (91.6%), Cephalothin (95.8%), Ampicillin sulbactam (91.6%), Nitrofurantoin (87.5%), where as isolates have shown lowest percentage of resistant to Chloramphenicol (12.5), Trimethoprime (20.83%). antagonistic activity of four types of probiotics bacteria against (24) isolates Escheriachia coli were performed. Results showed that all probiotics types have inhibition activity against E.coli isolates and the diameter of inhibition zone was highest (20.8 mm) when probiotic L.casei used ,while Bifido.bifidum showed less inhibitory activity against E.coli isolates with (14.5mm)of inhibition zone
In this study the Polymerase chain reaction technique (PCR) were used for detection of hemolysin gene (hly A). Two primers ( hlyA) with 561 bp and (hlyA) with 1177 bp were used for this purpose. The results were shown that only (62.5%) and (50%) of 24 E.coli isolates were carried the genes hlyA (561bp), hly A (1177bp) respectively. Furthermore the investigate of molecular influence of probiotics on hemolysin genes were done by using PCR. The results showed some of them lost hly A gene after treating with probiotics. In conclusion, hemolysin toxin gene is important virulence factor for uropathogenic E.coli and using PCR technique was appeared highly specific,very sensitive method, more than it serves as asuitable molecular diagnostic tool for detection UPEC producing hemolysin toxin .
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