Effect of Multi-Walled Carbon Nanotubes on lung tissue and concentration of enzyme Carbonic anhydrase in the New Zealand white rabbit
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Abstract
The aim of the present study was to investigate the effect of variable doses of Multi Carbon Nanotubes (MWCNTs) 1,3,5 mg/ml/kg body weight on lung weight and their effect on the concentration of Carbonic anhydrase(CA), morphological and histopathological changes of the males New Zealand white rabbits, In this study was used 40 New Zealand white rabbit of males, their ages range between (12-18 months) divided into four groups, ten for each group which intratracheal instillation with Nanoparticles, At days 7 and 90 post exposure, The blood , lung and trachea tissue samples were collected for each group.
The exposure with Multi Walled Carbon Nanotubes led to significant increase in the lung weight of all groups by increasing the dose and the exposure period compared with control group, While the effect of carbon nanotubes in the lung function was represented by estimating the concentration of enzyme Carbonic anhydrase in blood serum that led to significant increase in the concentration of the enzyme for all groups compared with control group, And the effect of carbon nanotubes were studied on morphological and histological changes in each of the lung and trachea of exposure rabbit compared with control group, the morphological and histological changes in the lung and trachea of exposure rabbit with MWCNTs after 7 days of exposure, included whiting the upper surface of the lobes, sever infiltration of the mixed inflammatory cells (mono and polymorphonuclear), hyperatrophy of the tissue, muscular hyperatrophy of bronchi and fibrosis of the region, while after 90 days of exposure, the histological change were similar to that occurred after 7 days of exposure but more clear. Thus it shows that the lung was most affected organ by this tubes, which approved by histological examination of lung.
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