Study of Leptin hormone concentration and other Immunological Parameters in Some People with Rheumatoid Arthritis in Kirkuk city
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Abstract
This research was designed for study the effect of rheumatoid arthritis diseases on Leptin hormone and some immunological parameters (Interleukins), This study involve 58 blood sample from 48 individual with rheumatoid arthritis compared with 10 healthy individual having no any health problems considered them as control group .
The estimation of secretes cytokines by leukocytes The interleukins (IL-1 α and IL- 6 ) was done using Enzyme Linked Immuno Sorbent Assay (ELISA) and the result of this study showed significant increase at significancy level (P<o.o5 ) in concentration of Immunological Interleukins (IL-1 α and IL- 6) in rheumatoid arthritis patients group in comparism with control group .
Also the results revealed significant increase significancy level (P<o.o5) of Leptin hormone concentration in rheumatoid arthritis patients group in comparism with control group .
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References
1. Gary S. Firestein.Evolving concepts of rheumatoid arthritis. Nature Puplishing Group. 2003; vol 423.
2. Gibofsky Allan,. Overview of Epidemiology, Pathophysiology, and Diagnosis of Rheumatoid Arthritis. Am J Manag Care. 2012;18:S295-S302.
3. McInnes Iain B., and Georg Schett. The Pathogenesis of Rheumatoid Arthritis. N Engl J Med 2011;365:2205-19.
4. Wassreman Amy M. Diagnosis and Management of Rheumatoid Arthritis. American Academy of Family Physicians.2011.
5. Lili Magyari, Dalma Varszegi, Erzsebet Kovesdi, Patricia Sarlos, Bernadett Farago, Andras Javorhazy,Katalin Sumegi, Zsolt Banfai, Bela Melegh . Interleukins and interleukin receptors in rheumatoid arthritis: Research, diagnostics and clinical implications. WJO 5th Anniversary Special Issues (10): Rheumatoid arthritis, 2014 September 18; 5(4): 516-6 ISSN 2218-5836.
6. Rneste h. S Choy and Gabrial S. Panayi. Cytokine Pathway and Joint Inflammation in Rheumatoid Arthritis. N Engl J Med, 2001, Vol. 344, No. 12.
7. Muraoka S, Kusunoki N, Takahashi H, Tsuchiya K, Kawai S. Leptinstimulates interleukin-6 production via janus kinase 2/signal transducer and activator of transcription 3 in rheumatoid synovial fibroblasts. Clin Exp Rheumatol 2013; 31:589–95.
8. Rhee DK, Marcelino J, Baker M. The secreted glycoprotein lubricin protects cartilage surfaces and inhibits synovial cell overgrowth. J Clin Invest 2005; 115:622-31.
9. Bokarewa M, Bokarew D, Hultgren O, Tarkowski A. Leptin consumption in the inflamed joints of patients with rheumatoid arthritis. Ann Rheum Dis 2003; 62:952–6.
10. Otero M, Lago R, Lago F. Leptin, from fat to inflammation: old questions and new insights. FEBS Lett 2005; 579:295– 301.
11. MacDougald OA, Hwang CS, Fan H, Lane MD. Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes. Proc Natl Acad Sci USA 1995; 92:9034–7.
12. Matarese G, Carrieri PB,Montella S, De Rosa V, La Cava A. Leptin as a metabolic link to multiple sclerosis. Nat Rev Neurol 2010; 6:455–61.
13. Rey LK, Wieczorek S, Akkad DA, Linker RA, Chan A, Hoffjan S.Polymorphisms in genes encoding leptin, ghrelin and their receptors in German multiple sclerosis patients. Mol Cell Probes 2011; 25:255–9.
14. Roth B, Manjer J, Ohlsson B. Microscopic colitis is associated with several concomitant diseases. Drug Target Insights 2013; 7:19–25.
15. Agata Burska, Marjorie Boissinot, and Frederique Ponchel. Cytokines as Biomarkers in Rheumatoid Arthritis. Hindawi Publishing Corporation Mediators of Inflammation ,Volume 2014, Article ID 545493, 24 pages.
16. Sugioka Y, Tada M, Okano T, Nakamura H, Koike T. Acquired leptin resistance by high-fat feeding reduces inflammation from collagen antibody-induced arthritis in mice. Clin Exp Rheumato 2012; 30:707–13.
17. Yoshino T, Kusunoki N, Tanaka N et al. Elevated serum levels ofresistin, leptin, and adiponectin are associated with C-reactive protein and also other clinical conditions in rheumatoid arthritis. Intern Med 2011; 50:269–75.
18. Xibille - Friedmann D, Bustos- Bahena C, Hernandez - Gongora S, Burgos - Vargas R, Montiel-Hernandez JL. Two-year follow-up of plasma leptin and other cytokines in patients with rheumatoid arthritis. Ann Rheum Dis 2010; 69:930–1.
19. Rho YH, Solus J, Sokka T. Adipocytokines are associated withradiographic joint damage in rheumatoid arthritis. Arthritis Rheum 2009; 60:1906–14.
20. Otero M, Lago R, Gomez R. Changes in plasma levels of fatderived hormones adiponectin, leptin, resistin and visfatin in patients with rheumatoid arthritis. Ann Rheum Dis 2006; 65:1198–201.
21. Anders HJ, Rihl M, Heufelder A, Loch O, Schattenkirchner M. Leptin serum levels are not correlated with disease activity in patients with rheumatoid arthritis. Metabolism 1999; 48:745–8.
22. Mohammad-Shahi M, Haidari F, Rashidi B, Saei AA, Mahboob S,Rashidi MR. Comparison of the effects of genistein and daidzein with dexamethasone and soy protein on rheumatoid arthritis in rats. BioImpacts 2011; 1:161–70.
23. Otero M, Lago R, Lago F, Reino JJ, Gualillo O. Signalling pathwayinvolved in nitric oxide synthase type II activation in chondrocytes: synergistic effect of leptin with interleukin-1. Arthritis Res Ther 2005; 7:R581–91.
24. Otero M, Gomez Reino JJ, Gualillo O. Synergistic induction of nitric oxide synthase type II: in vitro effect of leptin and interferon-gamma in human chondrocytes and ATDC5 chondrogenic cells. Arthritis Rheum 2003; 48:404–9.
25. Targonska - Stepniak B, Majdan M, Dryglewska M. Leptin serum levels in rheumatoid arthritis patients: relation to disease duration and activity. Rheumatol Int 2008; 28:585–91.
26. Figenschau Y, Knutsen G, Shahazeydi S, Johansen O, Sveinbjornsson B. Human articular
chondrocytes express functional leptin receptors. Biochem Biophys Res Commun 2001; 287:190–7.
27. Tian G M, Liang J.-N, Wang Z.-Y,and Zhou D. Emerging role of leptin in rheumatoid arthritis. British Society for Immunology, Clinical and Experimental Immunology, 2014; 177: 557–570.
28. Adewale, Adebago. ABC of Rheumatology. fourth edition , wlley – blackwell .2010; : 85-89.
29. Jhon R .Crewther. The ELISA Guid book. Humana Press Inc. 2001 ; 26-28.
30. Gerald Van Belle, Lloyd D. Fisher, Patrick J. Heagerty, Thomas Lumley. Biostatistics A Methodology for the helth sciences . second edition , welley interscience ; 2004 .
31. Shahlaa M. Saleh and Jabbar R. Zangor. Proinflammatory cytokines profile in patients with Rheumatoid Arthritis. J Fac Med Baghdad , 2009; vol.51 ,No . 1.
32. Yuji Yoshida, Toshio, and Tanaka. Interleukin 6 and RheumatoidArthritis. Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 698313, 12 pages.
33. Ernest Choy. Understanding the dynamics: pathways involved in the pathogenesis of rheumatoid arthritis. Rheumatology 2012;51:v3_v11. 34. Clifton O. Bingham. The Pathogenesis of Rheumatoid Arthritis: Pivotal Cytokines Involved in Bone Degradation and Inflammation. J Rheumatol 2002;29 Suppl 65:3-9.
35. Falah S. Manhal. Cytokine Profile in Patients with Rheumatoid Arthritis. Fac Med Baghdad . 2009; Vol. 51, No. 4.
36. Peter K. K. Wong, Ian K. Campbell, Paul J. Egan, Matthias Ernst, and Ian P. Wicks . The Role of the Interleukin-6 Family of Cytokines in Inflammatory Arthritis and Bone Turnover. Arthritis & Rheumatism , Vol. 48, No. 5, May 2003, pp 1177–1189.
37. Misato Hashizume and Masahiko Mihara . The Roles of Interleukin-6 in the Pathogenesis of Rheumatoid Arthritis. Hindawi Publishing Corporation Arthritis. 2011, Article ID 765624, 8 pages.