Spectrophotometric determination of Folic acid (B9) by oxidative Coupling Reaction with 4-nitro-aniline
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Abstract
Development of an easy, inexpensive, fast and selective spectrophotometric method for the determination of Folic Acid (FA) by its reaction with 4-nitro aniline reagent, with good sensitivity, as an oxidative coupling method in the presence of Potassium permanganate solution, and it was found that the concentrations that were agreement with Beer-Lambert’s law at the wavelength of 374 nm ranged between 1- 21 µg/ml of folic acid solution, and a deviation from Beer’s law occurs at a concentration higher than 21 µg/mL, gave a linear coefficient and correlation coefficient its value is 0.9994, 0.9996, respectively, While the value of the molar absorption coefficient 12138.5 L/mol.cm and Sandel's sensitivity 0.0363 gm/cm2 with the limits of qualitative and quantitative detection 0.103 and 0.363 μg / ml-1, respectively. The proposed method was successfully applied for the determination of folic acid in its pharmaceutical preparations, from Erbil Company – Iraq. Trade name (folic acide).
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References
[1] Modupe O., Krishnaswamy K., Diosady L.L. Technology for triple fortification of salt with folic acid, iron, and iodine. J. Food Sci. 2019
[2] Delchier, N.; Herbig, A.-L.; Rychlik, M.; Renard, C.M. Folates in Fruits and Vegetables: Contents, Processing, and Stability. Compr. Rev. Food Sci. Food Saf., 15, 506–528 ,2016.
[3] Gazzali A.M., Lobry M., Colombeau L., Acherar S., Azaïs H., Mordon S. Stability of folic acid under several parameters. Eur. J. Pharmaceut. Sci.;93:419–430,2016 .
[4] Yin S., Yang Y., Li Y., Sun C. Analysis of natural and synthetic folates in pharmaceuticals and foods: a review. Analy. Methods;10(1):9–21. 2018.
[5] Andlid, T.A.; D’ Aimmo, M.R.; Jastrebova, J.” Folate and bifidobacteria. In the Bifidobacteria and Related Organisms; Elsevier: Amsterdam, The Netherlands,; pp. 195–212,2018..
[6] Maharaj, P.P.; Prasad, S.; Devi, R.; Gopalan, R. Folate content and retention in commonly consumed vegetables in the South Pacific. Food Chem., 182, 327–332,2015..
[7] Striegel, L.; Weber, N.; Dumler, C.; Chebib, S.; Netzel, M.E.; Sultanbawa, Y.; Rychlik, M. Promising Tropical Fruits High in Folates. Foods, 8, 363,2019..
[8] López-Nicolás, R.; Frontela-Saseta, C.; González-Abellán, R.; Barado-Piqueras, A.; Perez-Conesa, D.; Ros, G. Folate fortification of white and whole-grain bread by adding Swiss chard and spinach. Acceptability by consumers. LWT, 59, 263–269,2014.
[9] Altic, L.; McNulty, H.; Hoey, L.; McAnena, L.; Pentieva, K. Validation of Folate-Enriched Eggs as a Functional Food for Improving Folate Intake in Consumers. Nutrition. 8, 777, 2016.
[10] Riyadh R, Muthana S, Emad A,” Spectrophotometric Determination of Vitamin Folic Acid B9 in Some Drugs Using 1,2-Naphthoquine-4-Sulphonate (NQS)”, Nano Biomed. Eng, , Department of Chemistry, Faculty of Science, University of Kufa , Jul. 19, 2017.,
[11] Oluwasegun M, Julie B, Levente L,” A spectrophotometric method for determining the amount of folic acid in fortified salt “ , Journal of Agriculture and Food Research , 2020 Dec; 2
[12] Marcos Vinícius . “Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry “Braz. J. Pharm. Sci. 52 (4) • Dec 2016
[13] Matias, R.; Ribeiro, P. R. S.; Sarraguça, M. C.; Lopes, J. A. “UV spectrophotometric method for the determination of folic acid in pharmaceutical tablets and dissolution tests “, Journal Article, 2014
[14] Multi w .” carbon nanotubes/nickel hydroxide composite applied aselectrochemical sensor for folic acid (vitamin B9) in food samples” Journal of Food Composition and AnalysisVolume 92, , 103511, September 2020
[15] Asmaa m R, Khalid A, Gassim M, Spectrophotometric Determination of Sulphamethoxazole Drug by New Pyrazoline Derived from 2,4-Dinitro Phenyl Hydrazine. 2020 Jul ; 1002 : 350-359.
[16] Abd Alrahman K A , Asmaa A M . Faisal S. 2nd International Conference on Materials Engineering & Science (IConMEAS 2019 Seb 25-26.