Development of Spectrophotometric method for quantitative determination of dexamethasone in pharmaceutical preparations
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Abstract
This Study is concerned a fast, easy, sensitive, accurate and economical spectrophotometric method for the determination of Dexamethasone. This method based on the use of UV- spectra measurements at 242 nm.
The linearity of the method is performed between (3 - 65) μg.ml-1, the coefficient of correlation was = 0.9998. The value of Rec% and RSD% are found at the range (95.28 -102.86)% and (0 - 0.272 )% respectively, The LOD and LOQ were (0.024and 0.081) μg.ml-1, respectively .
The method was successfully applied to the determination of DMT in Bulk and pharmaceutical preparations
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References
1- Victor A. Rogozkin . Metabolism of Anabolic-Androgenic Steroids. CRC Press, 1991; pp:1-2.
2- Klaus Urich. Comparative Animal Biochemistry. Springer Science & Business Media, 1994; pp: 624.
3- "Dexamethasone". The American Society of Health-System Pharmacists. Retrieved, 2015.
4- Yalkowsky SH, He Yan, Handbook of Aqueous Solubility Data: An Extensive Compilation of Aqueous Solubility Data for Organic Compounds Extracted from the aquasol database. CRC Press LLC, Boca Raton, FL, 2003; pp: 1197
5- "Prescribing medicines in pregnancy database". Australian Government, 2014.
6- Brady CJ, Villanti AC, Law HA, et al. "Corticosteroid implants for chronic non-infectious uveitis". Cochrane Database Syst Rev 2, 2016.
7- Harousseau JL, Attal M, Leleu X, et al. "Bortezomib plus dexamethasone as induction treatment prior to autologous stem cell transplantation in patients with newly diagnosed multiple myeloma:
results of an IFM phase II study".Haematologica . 2006;91 (11),pp: 1498–505.
8- Rossana BF, Aline R, Luiz CC, Clarice MBR, Ruy CRB "Validation of a simple and rapid UV spectrophotometric method for dexamethasone assay in tablets" Quím. Nova, 2009;32 (4),pp: 1052-4 .
9- Kashyap Shah, Prasanna Ku, Pradhan , Shreya RS "Analytical Method Development and Validation for Simultaneous Estimation of Ondansetron and Dexamethasone In Synthetic Mixture" J Pharm Biomed Sci,2014;04(05),pp:448-458.
10- Karanam R, Sireesha, Katakam P, HPLC-UV Method for simultaneous determination of ofloxacin and dexamethasone sodium phosphate, Int J Pharm Pharm Sci, 2012; 4(1),pp:415-418.
11- Ankit A, Subhash D, Sunil KT, Kashyap N, Method Development and its Validation for Quantitative Simultaneous Determination of Dexamethasone and Gatifloxacin in Ophthalmic Solution by RP-HPLC, Int. J. Med. Pharm. Res,2013; 1(1),pp:139‐144.
12- Mohan GB, Lakshmana AR and. Enka TR, Validated Spectrophotometric and stability indicating RP-HPLC methods for the simultaneous estimation of gatifloxacin and Dexamathasone in ophthalmic dosage form, Int. J. Chem. Sci. 2016; 14(2),pp: 617-634.
13- Rangaraju D, Patel S, Sambrekar A, Karigar A, Nagesh C, Development and validation of UV spectroscopic methodsfor simultaneous estimation of ofloxacin and dexamethasone sodium phosphate, UJPB, 2013; 01 (02),pp: 37-39.
14- Ali M, Akhoundi-Khalafi and Masoud RS, A New Technique for Quantitative Determination of Dexamethasone in Pharmaceutical and Biological Samples Using Kinetic Spectrophotometric Method, Int.j. And. chem, , 2015; p: 6.
15- Vaghasiya V, and Dr. Kashyap R, First order derivative method development and validation for simultaneous estimation of atropine sulfate and dexamethasone, ejpmr, 2016;3(4),pp:367-372.
17- Bai G, Wang Y, Armenante PM. “Velocity profiles and shear strain rate variability in the USP Dissolution Testing Apparatus 2 at Different Impeller Agitation Speeds, ” ijpharm, 2011; 403 (1-2) ,pp: 1-14.
18- Kortejärvi H, Malkki J, Marvola M, et al. "Level A In Vitro-In Vivo Correlation (IVIVC) Model with Bayesian Approach to Formulation Series". J Pharm Sci. 2006; 95 (7),pp:1595-1605.
19- British Pharmacopoeia, The Department of Health, London: The Stationary of ice. 2012; Volume
ӀӀӀ, pp:2706.
20- United States Pharmacopoeia, 2004;27th ed. The USP Convention-Inc., Rockville, MD.
21- Rossana BF, Aline R, Luiz CC, Clarice MBR,Ruy CRB, Validation of a simple and rapid UV spectrophotometric method for dexamethasone assay in tablets, Quím. Nova, 2009;.32.4.
22- Worapan P, PhD, Dissapong J, Waraporn T, A modified quenchers extraction for the determination of Dexametasone , ESJ, 2014;10 (33),pp:138-144.