Astudy of organic solvents to obtain bio-products from two genus of cyanobacteria, Anabaena sp. 65, Nostoc sp. 69 and identification of toxins in these bio-products by Hplc/Ms technique
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Abstract
This study was conducted to determine the effect of the types of organic solvents (Ethanol, Methanol, Diethyl ether, Acetone and water) on the amount of bio-products derived from two genus of cyanobacteria (Anabaena sp. Tu 65 and Nostoc sp. Tu 69) who were isolated and growth on selective media ASM-1 under temperature at (26) °C and pH (7.6) under continuous lighting intensity (2500) Lux for 30 days. The growth were measured by optical density by spectrophotometer at wave length 436 nm. The daily growth of the genus Anabaena sp. Tu 65 showed higher than that of the genus Nostoc sp. Tu 69.Extractionby organic solvents shown that the Methanol is the best solvent for extraction of bio-products from the two genus followed by Ethanol, Diethyl ether, Acetone and water respectively.
Identification the toxic compounds in the bio-products by HPLC_MS technique shown that the bio-products from the genus Anabaena sp. Tu 65, the presence of neurotoxin (anatoxin a (s)) as well as the presence of the compound ADDA (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid), one of the key parts for all types of Microcystins which considered as important of liver toxins produced by cyanobacteria, which, as the spectrum readings showed the presence of hepatic toxin [D-Asp3, Dha7] MCYST-LR is one of the types of Microcystins.
While the bio-products from the genus Nostoc sp. Tu 69 shown the presence of the compound (MCYST-YM (O)) which also one of the types of liver toxin Microcystin, as well as the presence of another hepatic toxin [D-Asp3, Dha7] MCYST-LR, whichis another type of Microcystins too, as well as the presence of neurotoxin Anatoxin a( s),also the probability of the presence of another neurotoxin Anatoxin, which appeared in the spectrum readings.
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