Isolation and diagnosis of some types of Gram negative bacteria from different sources of output at the Republican Educational Hospital in the city of Mosul

Main Article Content

Aeman Mohammed Khaleel
Hajur Ali Shareef
Hala Arshad Ali

Abstract

The study included the isolation and diagnosis of some Gram negative bacterial species from different sources of extraction (blood, vaginal swabs, wound swabs, burns, middle ear secretions and fractures) from patients in the Republican Education Hospital in Mosul for both sexes from August 2014 to February 2015 And in different ages.


The results showed isolating (21) type of Gram negative bacteria (86.7%). E.coli was the most common among isolates 38 isolates (45.6%) followed by Pseudomonas spp. 27 isolates and (31.86%) followed by Enterobactar spp. (22.6%), Klebsiella pneumonia 9 (10.62%), Proteus mirabilis 7 (8.26%), Acinetobacter bumannii 6 (7.08%), Serratia spp 4 (4.72%) and 2 isolates (2.36% ) Of the Raoutella spp. The bacterial species Providencia stuartii, Morganella morgana, Shigellaflexneri, Moraxella lacunatae, Hafnia alvi and Yersina were isolated by 1.2% each.


The results revealed that the isolates were the most isolated isolates from isolates, with isolates isolating 30 isolates and 36% followed by wound samples 28 (33.6%) and eye burns (28.6%). The percentage of isolation in the samples (vaginal swabs, fractures and purulent secretions of the middle ear) was 15 (18%), 12 (14.4%) and 7 (8.4%), respectively.


In the investigation of the dominance of the bacterial species in the source of the isolation, E.coli was found to be found in the samples of the citrus, followed by Enterobacter spp isolates, while in the samples of burns, the dominance of Pseudomonas spp isolates was followed by acinetobacter bumannii isolates. In the wounds samples, Pseudomonas spp isolates and Enterobacter spp isolates Eucoli isolates were more frequent, while the isolates of Pseudomonas spp were larger by 6%, followed by E. coli, Klebsiella pneumonia by 8.4%. Within the samples of the respiratory secretions, Pseudomonas spp .

Article Details

How to Cite
Aeman Mohammed Khaleel, Hajur Ali Shareef, & Hala Arshad Ali. (2023). Isolation and diagnosis of some types of Gram negative bacteria from different sources of output at the Republican Educational Hospital in the city of Mosul. Tikrit Journal of Pure Science, 22(8), 68–73. https://doi.org/10.25130/tjps.v22i8.854
Section
Articles

References

1. Sepehri G, Nejad HZ, Sepehri E, Razban S.

(2010). Bacterial profile and antimicrobial resistance

to commonly used antimicrobials in intra-abdominal

infections in two teaching hospitals. Am J Applied

Sci.;7:38-43.

2. Karlowsky JA, Jones ME, Thornsberry C, et al.

(2003a). Trends in antimicrobial susceptibilities

among Enterobacteriaceae isolated from hospitalized

patients in the United States from 1998 to 2001.

Antimicrob Agents Chemother; 47:1672–80.

3. Karlowsky JA, Draghi DC, Jones ME, et al.

(2003b). Surveillance for antimicrobial susceptibility

among clinical isolates of Pseudomonas aeruginosa

and Acinetobacter baumannii from hospitalized

patients in the United States, 1998 to 2001.

Antimicrob Agents Chemother;47:1681–8.

4. Khalili H., Soltani R., Afhami S., Dashtikhavidaki

S. and Alijani B.(2012). Antimicrobial

resistance pattern of gram-negative bacteria of

nosocomial origin at a teaching hospital in the Islamic

Republic of Iran. East Mediterr Health

Journal.18(2):172-7.

5. Public Health England, (2014). Identifigation of

nterobacteriaceae. UK Standards for Microbiology

Investigation. ID 16 Issue 3. 2. http: //www. hpa. org.

uk/SM/pdf.

6. Nataro, J. P. and Kaper, J. B. (1998). Diarrhe- a

genic Escherichia coli. Clin microbial Rev, 11, 142,

201.

7. Paterson DL. (2006). Resistance in gram-negative

bacteria: Enterobacteriaceae. AM J Med; 119: 20-28.

8. Mishra, S. K.; Acharya, J.; Kattel, H. P.; Koirala,

J.; & Rijal, B. P. (2012). Metallo-beta-lactamase

producing gram-negative bacterial isolates. Journal of

Nepal Health Research Council. 10(22), page 208-

213.

9. Koneman E, W.; Allen S. D.; danda W. M,

Schreck enberger pc and winn, WC. (1997). Color

atlas and text book of diagnostic microbiology. 5th

(ed). Lippin- cot- Raven publisher, philadephia, USA.

10. Salih ,M.K. Alrabadi, N.I., Thalij, K.M. and

Hussien, A.S.(2016).Isolation of Pathogenic Gram-

Negative Bacteria from urinary Tract infected

Patients.open Journal of Medical Microbiology,6:59-

65.

12. Shill, M.C., Huda, N.H., Moain, F.B. and

Karmakar, U.K. (2010) Prevalence of Uropathogens

in Diabetic Patients and Their Corresponding

Resistance Pattern: Results of a Survey Conducted at

Diagnostic Centers in Dhaka, Bangladesh. Oman

Medical Journal, 25, 282-285.

13. Hashim, B., Husin S. and Rahman, M.M.(2009).

Genetic analysis of extended spectrum B-lactamase

producing bacteria in hospital Kulallumpur,

Malaysia. Int J. Biores., 2:17-21.

14. Bano, K., Khan, J., Begum, H., Munir, S., Akbar,

N. and Ansari, J.A. (2012) Patterns of Antibiotic

Sensitivity of Bac-terial Pathogens among Urinary

Tract Infections (UTI) Patients in a Pakistani

Population. African Journal of Microbi-ology

Research, 6, 414-420.

15. Chukwuani, C.M., Onifade, M. and Sumonu, K.

(2002) Survey of Drug Practices and Antibiotic

Prescribing Pattern at a General Hospital in Nigeria.

Pharmacy World and Science, 24, 188-195.

18. Alghalibi, S. M. S.; Humaid, A.A.; Alshaibani, E.

A. S. and Alhamzy, E.H. L.(2011).Microorganisms

associated with burn Wound infection in sanaa,

Yemen.Egypt. Acad.J.Biolog.Sci.3(1):19-25.

19. Vural, M.K.; Altoparlak, U.; Celebi, D. and

Akcay, M.N.(2013).Comparison of Surface Swab and

Quantitative Biopsy Cultures Dependent on Isolated

Microorganisms from Burn Wound. the Eurasian J.

Medicine., 45:34-38.

20. Tanela, N.; Chari, P.S.; Singh, M.; Singh, Biswal,

M. and Sharma, M.(2013).Evolution of bacterial flora

in burn wound :keyrole of environmental disinfection

in control of infection.Int. J.Burn Trauma.3(2):102-

107.

21. Asghar AH, Faidah HS.( 2009) Frequency and

antimicrobial susceptibility of gram-negative bacteria

isolated from 2 hospitals in Makkah, Saudi Arabia.

Saudi Med. J.; 30(8); 79-185.

22. Okon K.O, Blogun, S.T, Askira U.M, Jibrin Y.B,

Aguoru C.U, Isyaka T.M, and Ghamba

P.E.(2014).Retrospective Analysis of Gram Negative

Bacteria Isolation at aTertairy Hospital in Maiduguri,

Niegeria, British Microbiology Research Journal

4(11):1235-1247.

23. Studemeister, A. (2013). Community-Acquired

Acinetobacter baumannii Infections in Northern

California. Clin Microbial 2: 126. doi: 10. 4172/2327-

5073. 1000126

26. Rasheed, J. K.; Kitchel, B.; Zhu, W.; Anderson,

K. F.; Clark, N. C.; Ferraro, M. J.; ... & Limbago, B.

M. (2013). New Delhi Metallo-β-Lactamase–

producing Enterobacteriaceae, United States.

Emerging infectious diseases, 19(6), 870.

27. Altun, S.; Tufan, Z. K.; Yağcı, S.; Önde, U.;

Bulut, C.; & Kiniki, S. (2013). Extended spectrum

beta-lactamases, AmpC and metallo beta-lactamases

in emerging multi-drug resistant Gram-negative

bacteria in intensive care unit. Sci Rep, 2(4), 707.

28. Alabi A, Frielinghaus L, Kaba H, Koster K

(2013). Retrospective analysis of antimicrobial

resistance and bacterial spectrum of infection in

Gabon Central Africa. BMC Infection Diseases.; 13;

455.

29. Elaha Tajbakhsh; Sara Tajbakhsh and Faham

Khamesipour. (2015).Isolation and Molecular

Detection of Gram Negative Bacteria Cousing

Urinary Tract infection in Pationts Referred to

Shahrekord Hospitals .Research Articale ,Iran.(5) 17.

30. Ramsamy Y., Muckart DJ, Han KS.(2013).

Microbiology surveillance and antimicrobial

stewardship minimize the need for ultrabroad –

spectrum combination therapy for treatment of

nosocomial infection in atrauma intensive care unit ;

an audit of an evidence-based empiric antimicrobial

policy.Safr Med J; 103(6):371-6.

31. Simranjit K. and Prashant chauhan. (2015).

Isolation and characetrisation of pathogens from

various clinical Samples : A step toward prevention

of Infection Diseases.Journal of pharmaceutical

Science and Bioscentific Research (JPSBR).5(4):404-

409.