Effect of Some Plants Extracts on the Viability of Protoscoleces in Vitro
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Abstract
This study was conducted to evaluate the protoscolicidal effect of four plants alcoholic extracts on protoscoleces of the parasite Echinococcus granulosus in vitro. The plants that had been used in this study were: Viscum album (Mistletoe) and Prunus armeniaca (Apricot bitter kernels) with concentrations of 375, 750 and 1500 mg/ml, also Syzygium aromaticum (Clove flower buds) and Capsicum frutescence (Chili pepper) with concentrations of 375, 750 and 1500 µg/ml. All extracts were treated at four periods (15, 30, 45 and 60 min.).
In vitro, the high percentage of protoscoleces killing was observed in V. album alcoholic extract (80.7%) in the high concentration 1500 mg/ml at 60 min., and the lowest percentage of protoscoleces killing (62%) was in the concentration 375 mg/ml at 15 min.
- armeniaca alcoholic extract high percentage of killing (70.2%) was also at the high concentration 1500 mg/ml at 60 min., and the lowest percentage of protoscoleces killing (50.4 %) at the concentration 375 mg/ml at 15 min.
The high percentage of protoscoleces killing of alcoholic extract of S. aromaticum (73.7%) was at the concentration 1500 µg/ml at 60 min. and the lowest percentage (58.7%) was at the concentration 375 µg/ml at 15 min.
- frutescence alcoholic extract high percentage of protoscoleces killing (71.4 %) was at the concentration 1500 µg/ml at 60 min. and the lowest percentage (40.6 %) was at the concentration 375 µg/ml at 15 min.
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References
1- Fasihi, H.M.; Budke, C.M. and Rostami, S. The
Monetary Burden of Cystic Echinococcosis in Iran.
PLOS Negl. Trop. Dis., 2012; 6 (11): e1915.
2-Turcekova, L.; Snabel, V.; Dudinak, V.; Gasper, V.
and Dubinsky, P. Prevalence of cystic Echinococcosis
in pigs from Slovakia, with evaluation of size,
fertility and number of hydatid cysts. Helminthologia,
2009; 4 (3): 151-158.
3-Zulfikaroglu, B.; Koc, M.; Ozalp, N. and Ozmen,
M.M. A rare primary location of echinococcal
disease: report of a case. Upsala J. Med. Sci., 2005;
110: 167-171.
4- Smego Jr., R.A. and Sebanego, P. Treatment
options for hepatic cystic echinococcosis.
International Journal for Infectious Diseases, 2005; 9:
69–76.
5-Smyth J.D. and Barret N.J.: Procedure for testing
the viability of human hydatid cysts following
surgical removal, especially after chemotherapy.
Trans. Roy. Soci. Trop. Med. Hyg., 1980; 74 (5):
649-652.
.80-68 :( 3) 02 ;0202،
7-Abbas, S.K.; Abbas, A.K. and Zabbon A.A. The
efficiency of Cinnamomum zeylanicum oil extract on
some physiological and histological aspects in mice
infected with hydatid cysts of Echinococcus
granulosus. Iraqi J. Sci., 2014; 55 (3): 1196-1207.
8- Obiad, H. M.; Al-Alousi, T. I. and Al-Juboori A.
H. The in vivo effect of some medicinal plant extracts
on Cryptosporidium parasite. Journal of University of
Anbar for Pure Science, 2012; 6(3): 1-12.
9- Al-Bakri, S.A., Nima, Z.A., Jabri R. R. and Ajeel
E. A. Antibacterial activity of apricot kernel extract
containing amygdalin. Iraqi J. Sci, 2010; 51(4): 571-
576.
10- Yiğit D., Yiğit N. and Mavi A., Antioxidant and
antimicrobial activities of bitter and sweet apricot
(Prunus armeniaca L.) kernels, Braz. J. Med. Biol.
Res., 2009; 42(4): 346-352.
11-Barnes, D.A; Bannin, A.; Huaung, J.X. A novel
multi domain mucin-like glycoprotein of C. parvum
mediates invasion. Mol. Biochem., Parasitol., 1998;
96 (1-2): 93-110.
12- Chatterjee, A.; Banerjee S.; Steffen M.;
O’Connor, R. M.; Ward, H. D.; Robbins P. W. and
Samuelson J. Evidence for Mucin-Like Glycoproteins
That Tether Sporozoites of Cryptosporidium parvum
to the Inner Surface of the Oocyst Wall. Eukaryotic
Cell, 2010; 9 (1): 84–96
13- Diaz, A., Fontana, E.C., Todeschini, A.R., Soule,
S., Gonzalez, H., Casaravilla, C., et al. The major
surface carbohydrates of the Echinococcus
granulosus cyst: mucin-type O-glycans decorated by
novel galactose-based structures. Biochemistry, 2009;
48: 1678–1691.
14- Diaz, A.; Frnandez, C.; Pittini, A. and Allen, J.
The laminated layer: Recent advances and insights
into Echinococcus biology and evolution. Exp.
Parasitol., 2015; 158: 23-30.
15-Chen Y, Ma J, Wang F, Hu J, Cui A, Wei C, et al.
Amygdalin induces apoptosis in human cervical
cancer cell line HeLa cells. Immunopharmacol.
Immunotoxicol., 2013; 35: 43‑51.
16-Manoj D. K. and Veerakumari L. In Vitro Studies
on the Effect of Ethanol Extract of Syzygium
aromaticum on the Carbohydrate Metabolism of
Cotylophoron cotylophorum. Inter. J. Adv. Vet. Sci.
and Tech., 2015; 4 (1): 199-210.
17- Kumar and Singh. Activity of Allium sativum,
Ferula asafoetida and Syzygium aromaticum against
Fasciola gigantica. Journal of Biology and Earth
Sciences, 2014; 4 (1): B57-B65.