Evaluation of T-lymphocytes in Peripheral Blood of Diabetic Patients
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Abstract
Our study includes the estimation of absolute number of T-lymphocytes by using (E-rosette) test in diabetic patients with hyperglycemia. The study included the collection of (20 )blood samples from individuals Type I ,II diabetes mellitus, ten of them were of type one I others of type II, with age range between (8-55)years and of both sexes. Blood samples from healthy individuals as control samples were used as well. The study showed a significant decrease in absolute numbers of T-lymphocytes in diabetic patients when compared with control and also a significant reduction in the absolute number in type I and type II diabetic patients compared with control, our result suggest that there is a defect in T-lymphocytes numbers in diabetic patients with hyperglycemia.
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References
1- Mac Cuish, A.; Irvine, W.; Barnes, E.; Duncan,
L.(1974). Antibodies to pancreatic islet cells in
insulin-dependent diabetics with coexistent
autoimmune disease. Lancet 2:1529-31.
2- Ganda, O.P.; Srikanta, S.; Brink ,S.J.; Morris,
M.A.; Gleason, R.E.; Soeldner, J.S.; Eisenbarth,
G.S.(1984) Differential sensitivity to p-cell
secretagogues in "early, "type I diabetes mellitus.
Diab.33:516-21.
3-Dubois, H.F.; Bankauskaite, V.(2005). Type 2
diabetes programmers in Europe. Euro Observer 7:
5–6.
4-Ledbetter, J.; Evans, R.;Lipinski, M.; Cunningham-
Rundles, C.; Good, R.; Herzenberg, L. (1981)
Evolutionary conservation of surface molecules that
distinguish T lymphocyte helper/ inducer and
cytotoxic/suppressor subpopulations in mouse and
man. J Exp Med 153:310-23.
5- Habib, A.G.; Gebi, U.I.; Sani, B.G.; Maisaka,
M.U.; Oyeniyi, T.; Musa, B.O.; Onyemelukwe, G.C.
(2008). Insulin dependent diabetes mellitus
complicating follicular lymphoma in an adult
Nigerian: immunobiological aspects. Intern. Diab.
Dig.; 7:334.
6- Ifere, O.G.(2009). Lymphocytes membrane protein
glycosylation: a possible cause of lowered immunecompetence
in diabetic subjects. Diab. Intern; 10:14-
15.
7- Jondal, M.; Holm, G.; Weigzell, H. (1972).
Surface markers on human T and B lymphocytes. J.
Exp. Med. J Exp Med, 1972; 136: 207-215.
8- Bagdade, J. D; Bulger. R. J. (2004). Impaired
leukocyte function in patients with poorly controlled
diabetes. Diab; 23:9-15.
9- Perillie, P. E.; Nolan, J. P. and Finch, S. C. (2000).
Studies of the resistance to infection in diabetes
mellitus: local exudative cellular response. J. Lab.
Clin. Med.; 59:1008-1015.
10-Tietz, N.W. (1995). Textbook of Clinical
Chemistry. 3ed. C.A. Curtis, E.R. Silverman, L.M.,
Christensen, R.H. pp:523-524.
11-Lewis, S.A.; Bain, B.J.(2001). Dacie and lewis
practical haematology harcount publishers limited.
9th.ed.,pp 320.
12-Mckenzie, S.B. (1996). Textbook Of Hematology.
2eded., Williams and Wilkins, A Waverly company.
U.S.A. pp: 605-607.
13-Musa, B.O.P.; Onyemelukwe, G.C.; Hambolu,
J.O.; Bakari, A.G.; Anumah F.E. (2010). Cellmediated
immunity in type 2 diabetes mellitus
patients in diabetic ketoacidosis, patients with
controlled type2 diabetes mellitus and healthy control
subjects. J. Medicine and Med. Sci.1(7)pp: 290-295
©2010 international research Journals.
14-Knotek, Z.; Vojttsek, P.; Petra ladkov, A; Hoihn,
F; Kovai, O.; Madr, P.; Drabek, J.(1991).A simple
and efficient variant of the E- rosette test for the
detection of T- lymphocytes in pigs. J. Acta. Vet.
Brno.,80, pp:231-236.
15-VonKänel, R.; Mills, P.J.; Dimsdale, J.E.(2001).
Short-term hyperglycemia induces lymphopenia and
lymphocytes subset redistribution. J. Life Sci.2001
june 8;69(3):255-62.
16-Nerup, J.; Platz, P.; Andersen, O.O.; Christy, M.;
lyngsøe, J.; poulsen, J.E.; Ryder, L.P.; Nielsen, L.S.;
Thomsen, M.; Sveigaard, A. (1974). HLA antigen
and diabetes mellitus. J. Lancet. 2:864-66.
17- Mac Cuish, A.C.; Urbaniak, S.J.; Cambell, C.J.;
Duncan, L.J.P.; Irvine, W.J. (1974).
Phytohemagglutinin transformation and circulating
lymphocyte subpopulation in insulin-dependent
diabetic patients. J. Diab.23:708-12.
18- Pometkin, V.V.; Nikonova, T.V.; Brykova, S.V.
(1994). Dynamics of a series of parameter of cellular
and humoral immunity in patients with diabetes
mellitus type1. J.probl Endokrinal. (Mosk) 40(6)pp:5-
7.
19- Tujino, M.; Kinpara, I.; Nakamura, T.; Suda, T.;
Saitou, Y.; Kudou, H. (1996). Peripheral lymphocytes
subset of paitents with pancreatic diabetes mellitus –
about adecreased ratio of T- LGL and ability of host
defense. J. Kansenshogaku Zasshi .70(4):325-30.
20- Karachunskii, M.A.; Gergert, VIA.; Lakovleva,
O.B. (1997).Specific features of cellular immunity of
pulmonary tuberculosis in patients with diabetes
mellitus. J.Probl. Tuberk.(6)pp:56-60.
21-Tsukaguchi, K.; Okamura, H.; Ikumo, M.;
Kobayashi, A,l.; Fukuoka, A.; Takanaka, H. (1997).
The relation between diabetes mellitus and IFNgamma,
IL12 and IL-10 production by CD+4 alpha
beta T cells and monocytes in patients with
pulmonary tuberculosis. J. Kekkaku.72(11)617-22.
22-Chang, F.Y.; Shaio, M.F.(1995). Decreased cellmediated
immunity in patients with non- insulin
dependent diabetes mellitus. J. Diabetes Res Clin
Pract. 28(2)137-46.
23- Otton, R.; Mendonca, J.R.; Curi, R. (2002).
Diabetes causes marked changes in lymphocytes
metabolism. J. Endocrinology.174,pp:55-61.
24-Otton, R.; Soriano, F.G.; Verlengia, R.; Curi, R.
(2004). Diabetes induces apoptosis in lymphocytes. J.
Endocrinology.182.pp:145-156.
25-Plouffe, J.F.; Silva, J.; Fekety, R.; Allen, J.L.
(1978). Cell mediated immunity
In diabetes mellitus. J. Infection and Immunity.
21(2)pp: 425-429.