Molecular detection of some genes virulent bacteria Proteus mirabilis isolated from kidney stones
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Abstract
The current study Included the collection of samples (68) grit kidneys of patients undergoing surgery for lifting gravel in hospitals in the city of Tikrit and for the period from the month of April 2012 to the month of May 2013. The results of the study that people with the proportion of males (64.71%) while she was infected with the proportion of females (35.29 %) outweigh any male female ratio (1.83:1), diagnostic results showed depending on the biochemical tests and API-20system showed that E.coli and Ps.aeruginosa were the most frequent among other bacterial species and by isolating (30.76%) and (21.22%), respectively, while the bacteria P.mirabilis has given isolate percentage (15.38%) Followed by bacteria and Kl.pneumoniae Citrobacter.freundii by insolation (9.6%) and (5.67%) respectively, while giving the rest of the bacterial species (Staph.aureus,, Acinetobacter. freundii, E.faecalis Serratia .marscenes) isolation rate equal (3.84%), while isolating the bacteria Staph.epidermidis did not exceed (1.92%).The results of the investigation of the presence of genes,( mrpA, zapA, ureC) using polymerase chain reaction DNA (PCR) , Revealed that all isolates bacteria of P.mirabilis owning genes ( mrpA, zapA, ureC) encoding virulence factors (urease, protease, the ability to produce pili (mrp).
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