Effect of oxidative stress and physiological parameters in use Nigella sativa aqueous extract and some Drugs in inhibition in induced renal calculi formation in Rat.
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Abstract
This study aimed to study the effects of physiological and biochemical changes ofthe aqueous extract Nigella sativaseeds. And using drugs (common usig in treatment of renal calculi) like Coli-Urinal, Uricol and Kellagon powders in inhibition of renal calculi formation which induced by Ethylene glycol. The study inducted to determination many concentration of it had been investigated for Total protein, Albumin, Globulin, Glutathione (GSH) and Maloddialdehyde (MDA) concentrations had been determined. The study involud The animals distributed and randomly divided to (6) groups (each group included 6 rats) as follow:- Group(1): (control group): Fed with normal diet and drinking water Daily. Group(2):The animals treated with (0.9% g\kg of body weight) Ethylene glycol Daily. Group(3): The animals treated with 1ml (0.9% g\kg of body weight) of Ethylene glycol and 2ml(10%) g\kg of body weight of aqueous extract Nigella sativa Daily. Group(4): The animals treated with 1ml (0.9% g\kg of body weight) of Ethylene glycol and 2ml (10%) g\kg of body weight of 1m (0.7%) g\kg of body weithg of Coli-Urinal drug Daily. Group(5):The animals treated with 1ml (0.9% g\kg of body weight) of Ethylene glycol and 2ml (10%) g\kg of body weight of 1m(0.10%) g\kg of body weight of Uricol drug Daily. Group(6):The animals treated with 1ml (0.9% g\kg of body weight) of Ethylene glycol and 2ml (10%) g\kg of body weight of 1m (0.10%) g\kg of body weight of kellagon drug Daily. The results revealed:-The treatment by (0.9% g\kg of body weight) Ethylene glycol caused high significant decrease (P≤0.01) of. Total protein, Globulin and Maloddialdehyde (MDA) compared with the control group. While the treatment by Ethylene glycol aqueous extract Nigella sativa, treated with of Ethylene glycol and of Coli-Urinal drug, treated with of Ethylene glycol and of Uricol drug and treated with of Ethylene glycol kellagon drug caused high significant increase of Total protein, Globulin and Maloddialdehyde (MDA) compared with the treatment by Ethylene glycol. Caused The treatment by Ethylene glycol high significant increase in concentration of Glutathione (GSH) compared with the control group, and treated with of Ethylene glycol kellagon drug caused caused high significant decrease in concentration of Glutathione (GSH). While the treatment by Ethylene glycol aqueous extract Nigella sativa, treated with of Ethylene glycol and of Coli-Urinal drug, treated with of Ethylene glycol and of Uricol drug There was no significant variations in Glutathione (GSH). There were no significant variations of Albumin in sera of all groups compared with compared with the control group and compared with the treatment by Ethylene glycol
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