Study of the aqueous extract impact of Vitex agnus castus on the female reproductive system hormones of the Albino Rabbit exposed to oxidative stress
Article Sidebar
Main Article Content
Abstract
In view of the wide use of the Vitex agnus castus in treatment of the monstrous troubles and as a regulation of the sexual Hormones, this study was conducted to know the impact of the aqueous extract of the Vitex agnus castus compared with Vitamin E on the sexual Hormones in the female white rabbits exposed to the hydrogen peroxide (0.5%) for (30) days and the rabbit were (6-8) months old and their weights (1300-1700) g. They were divided at random into (10) groups, each (5) rabbits to one group as following: (1) The health control group consumed the normal drinking water and the standard nutrition, (2) the infected control group consumed the hydrogen peroxide with drinking water and a nutrient, (3) the extract had a concentration (50mg/kg), (4) the extract(50mg/kg)+H2O2, (5) the extract (50mg/kg)+Vitamin E, (6) the extract (50mg/kg)+Vitamin E+H2O2, (7) Vitamin E, (8) the extract (100mg/kg), (9) the extract (100mg/kg)+H2O2, (10) the extract (100mg/kg)+Vitamin E+H2O2. The concentration of the sexual Hormones were estimated to know the impact of the aqueous extract of Vitex agnus castus on the functions and capacity of the sexual organ in the female rabbits. The results of study showed that giving these rabbits H2O2 with drinking water led to decrease significantly in concentrations of Progesterone, Estrogen, FSH, LH, Hormones, whereas they showed a significant increase in concentration of prolactin Hormone compared with the healthy control group. It was also showed a significant increase in Progesterone Hormone at the dose(50mg/kg) with Vitamin E compared with the healthy control group, as well as there was a significant decrease in concentration of LH Hormone in all groups compared with the healthy control except the Vitamin E group only that showed a significant increase(P<0.01), whereas the concentration of Prolactin Hormone decreased in all groups compared with the healthy and infected. So we conducted that the Vitex agnus castus had an important role in fertility and inhibition of the Prolactin Hormone as the main cause of injuring the women by infertility.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
1- Agarwal, V; Lal, P. and Pruthi, V. (2012).Effect of plant oils on Candida albicans. J Microbiol Immunol Infect. 43:447-451 .
- 1 Islam, MD.S., Akhtar, M.M., Parvez, MD.S., Alam, MD. S., Alam, M. F. (2013). Antitumar and antibacterial activity Acrude methanol leaf Extroct of Vitex negundo. Arch. Biol. Sci., Belgrade. 65(1). pp:229-238.
3- Townsend, C. C. and Guest, E. (1980). Flora of Iraq . Ministry of agriculture Republic of Iraq vol. 4.
4- Azarnia, M; Ejtemaei, S; Shakoora, A. and Ansari, A. (2007). Effect of Vitex agnus-castus on mice fetus development. Acta mediea Journal. 45(4): 264.
5- Mabberley, D. J.( 2008). Mabberleys' Plant-Book 3rd edition 2008 Cambridge University Press: UK.
6- Al-Rawi, A.(1988). Wild plants of Iraq (3rd ed.).Ministry of Agriculture and Irrigation, Repuplic of Iraq, Baghdad.pp:6, 149.
7- Ibrahim, N.A; Shalaby, A.S; Farag, R.S; Elbaroty, G.S; Nofal, S. and Hassan, E.M. (2007). Phytochemical Investigation and Hormonal Activity of Vitex agnus-castus Fruits growing in Egypt. Faculty of Agric., Cairo Univ., Egypt.
8- Burch, E.(1999). Vitex Agnus castus ,www @herbcraft.com.(cited by, Nasri 2004).
9- Khare, C. P. (2004). Indian medicinal plants, an illustrated dictionary. 2nd edition. Springer publishers. pp: 709-711.
10-Tareen, RB; Bibi, T; Kahn, MA; Ahmad, M. and Zafar, M.(2010). Indigenous Knowledge of Folk Medicin by the Women of Kalat and Khuzdar Regions of Balochistan, Pakistan, Pakistan Journal of Botany 42(3):1465-1485.
13- Milosavljevic, S; Tesevic, V; Vuckovic, I; Jadranin, M; Vajs, V; Sokovic, M; Janackovic, P. and Jovanovic, A. (2007). "Composition and antifungal activity of the essential oil of Seseli annuum wild-growing in Serbia". Fitoterapia. 78, 319-322.
14- Gidado, A; Amed, D. A. and Atawodi, S. E. (2005). Effect of Nauclea latitolia leaves aqueous extracts on blood glucose levels of normal and alloxan-induced diabetic rats. Atri. J. of. Bio technology. 4(1): 91-93.
15- Duncan, D.B.(1955). Multiple range and F-test. Biomertic. 11:42.
16- Dewey, K.G.(2001). Nutrition Growth and Complementary Feeding of the Breastfed Infant. Pedi clini of North American. 48(1).
17- Diab, A.A; Elsayed, Z.I; Zahra, M,H; Shalaby, A.A. and Mohamed, E.F.E. (2015). Biological study of the extract of some species of Vitex agnus-castus (kafmurium) grown in Egypt. International Journal of pharma sciences and Research. 6(2):227-233.
18- Liu, J; Burdette, JE; Xu, H., Gu, C; Van Bremeen, RB; Bhat, KP; Booth, N; Constantinou, AI; Pezzuto, JM; Fong, HH; Farnsworth, NR. and Bolton, JL.(2001). Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms. J Agric Food chem . 49(5):2472-2479.
19- Milewicz, A; Gejdel, E; Sworen, H; Sienkiewicz, K; Jedrzejak, J; Teucher, T. and Schmitz, H. (1993). Vitex agnus castus extract in the treatment of luteal phase defects due to latent hyperprolactinemia. Results of arandomized placebo-controlled double-blind study. Arzneimittelforscung. 43(7): 752-756.
20-Sergerson, E.C. and Ganapathy, S.N. (1980). Fertilization of ovine selenium /vitamin E treated ewes maintained on two planes of nutrition J. Anim Sci. 51:386-394.
21-Ibrahim, NA; Shalaby, AS; Farag, RS; Elbaroty, GS; Nofal, SM. and Hassan, EM. (2008). Gynecological efficacy and chemical investigation of Vitex agnus-castus L. fruit growing in Egypt. Nat Prod Res. 22(6):537-546.
22- Jarry, H; Spengler, B; Porzel, A; Schmidt, J; Wuttlue, W. and Christoffel, V. (2003).Evidence for estrogen receptor B-Selective activity of Vitex agnus-castus and isolated flavones. Plant Medica, 69K:645-647.
23- Amri, O; Elguiche, R; Tahrouch, S; Zekhnini, A. and Hatimi, A.(2015). Antifungal and antioxidant activities of some aromatic and medicinal plants from the Southwest of Morocoo. Journal of chemical and pharmaceutical Research. 7(7):672-678.
24- Cicel, N; Eryilmaz, OG; Sarikaya, E; Gulerman, C. and Yasemin, G. (2012). Vitamin E effect on Controled Ovarian stimulation of unexplained infertile women. J Assist Reprod Genet. 29: 25-328.
26- Nasri , S; Oryan , S; Haeri Rohani, A; Amin, GH. and Yahyavi , H.(2004). The Effects of Vitex agnus castus L. Extract on Gonadotrophines and Testosterone in Male Mice. Iranian Int. J. Sci. 5(1). p:25-30.
27-Warren, M.P. and Stiehl, A.L. (1999) Exercise and female adolescents: effects on the reproductive and skeletal systems. Journal of the American Medical Women’s Association 54 (3), 115-120.
28-Silberstein, S.D. and Merriam, G.R. (2000). Physiology of the menstrual cycle. Cephalalgia. (20):148-154.
29- Lauritzen, C; Reuter, HD; Repges, R; Bohnert, KJ. and Schmidt, U. (1997). Treatment of premenstrual tension syndrome with Vitex agnus castus controlled double-blind study versus pyridoxine. Phytomedicine. 4:183-189.
30- Soules, M.F. (1989). Problems in reproductive endocrindogy and infertility. Baltimore, Williams and Wilkins.
31-Umeda, F; Kato, K; Muta, K. and Ibayashi, H. (1982). Effect of vitamin E on function of pituitary-gonadal axis in male rats and human subjects. Endocrinol JPn. 29(3):287-292.
32-YE, Q; Zhang, QY; Zhang, CJ; Wang, Y. and Qin, LP.(2010). Casticin, a flavonoid isolated from Vitex rotundifolia , inhibits prolactin release in vivo and vitro. Act Pharmacol Sin.31(12):1564-1568.
33- Martin, A; Prior, R; Shukitt-Hale, B; Cao, G. and Joseph, J.A. (2015). Effect of fruits, Vegetables, or Vitamin E-Rich diet or Vitamins E and C distribution in peripheral and brain tissues. The Journal of Gerontology. 55(3).pp:B144-B151.