Biochemical and histological study of the effect of sterol extract from pistachio on the level of antioxidants in the liver tissue green
Main Article Content
Abstract
The effect of sterol extract of pistachio on the levels of anti- oxidant was carried out in liver tissue of adult male rabbit, twenty eight of local rabbite, weigh range(800-1500)g. The animals was divided randomly into 4 groups (7 rabbits in each group). three groups G1,G2 and G3 in was treated orally with (50,100,150) mg/kg of sterol extract of pistachio respectively for a period of 30 days, after the end of the experiment starved rabbits for 12 hours and then seduced by the use of ether after anesthesia animal transfer to a bowl anatomy as proven front and rear sides of the animal Baldbabis and then worked an opening in the the abdomen to the sternum was extracted liver tissue as it was saved in the solution Alveslchi was estimated antioxidants in liver tissue, including the glutathione(GSH) and malon dialdehyde (MDA). The results showed that the glutathion level significantly increased in liver tissue in all groups compared with control, while the MDA level significantly increased in liver tissue in G2,G3 compared with control, While the results of microscopic histological study showed that the treatment groups sterol extract raw green pistachios be free from the distortions in addition to the lack of a change in the nature of the liver tissue.
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
1-Azadmard damirchi, S.; Sh. Emami, J.; Hesari. et al. Nuts Compostion and their Health Benefits.
International Scholarly and Scientific Reasearch &Innovation. 2011; 5(9).
2- Gentile, C.; Tesoriere, L.; Butera, D., et al .Antioxidant activity of Sicilian pistachio(pistacia vera L. var. Bronte) nut extract and its bioactive components. J.Agric. food chem.2007;55:643-648.
3- Karimi H. R., Zamani Z., Ebadi, A.and Fatahi, M. R. “Morphological diversity of Pistacia species in Iran,” Genetic Resources and Crop Evolution, 2009; 56(4): 561–571.
4- Bolling, B.W, Chen, C.Y.; Mclcay, D.L and Blumbery, J.B. Tree nut phytochemicals: Composition, antioxidant capacity, bioactivity, impact 1 factors. A systematic review of almonds, Brazils, cashews, hazelnuts, macadamias, pecans, pine nuts, pistachios, and walnuts. Nutr Res Rev. 2011;24:244-275.
5-AnEvidence-Based, Analysis of the Relationship between Consumption of Tree Nuts and the Risk of Cardiovascular Disease, Life Sciences. Research Organization, December. 2013.
8 –Kendall, C.W; West, S.G.; Auqustin, L.S.; et al. Acute effects of pistachio consumption on glucose and insulin, satiety hormones and endothelial function in the metabolic syndrome. Eur J Clin Nutr. 2014; 68(3):370-375.
7-Albert CM, Nut consumption and decreased risk of sudden cardiac in the physicians health study .Arch intern Med.2002;162(12):1382-7.
8-Bao, Y.; Wolpin , B.M.; Giovannucci, E.L.; et al. Nut consumption and risk of pancreatic cancer in women. Br. J Cancer. 2013.
9- Kay, C,D.; Gebauer, S.K.; West ,S.G and Kris-etherton, p.m. Pistachios increase serum antioxidants and lower serum oxidized - LDL in hypercholesterolemic adults. J Nutr. 2010;140:1093-1098
10- Chen, A.F. ; Chen, D.D. ; Daiber, A.; Free radical biology of the cardiovascular system. Clin. Sci (Lond). 2012 ;123(2):73-91.
11-Halliwell, B. Free radicals and antioxidants: updating a personal. View .Nutr. Rev. 2012; 70(5): 257-65.
12-Fu , L.; Xu, B. T.; Xu, X. R.; Gan, R. Y.; Zhang, Y.; Xia, E. Q. and Li, H. B. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chem. 2011;129: 345-350.
13-Rowlands, D.S.; Pearce, E.; Aboud, A .Oxidative stress inflammation, and muscle soreness in an 894-km relay trail run. Eur. J. Appl Physiol 2011;112(5): 1839-48.
14-Rodrigo, R. Oxidative stress and Antioxidants. Nova Biomedical Books. New York. 2009:358.ROM. J. BIOL. – PLANT BIOL. Bucharest, 2010; 55( 2): 65–70.
15-Gu¨lc¸in, I.; Topal, F. C.; Akmakc, R.; Go¨ren, A.C.; Bilsel, M. and Erdog˘an, U. Pomological features, nutritional quality, polyphenol content analysis and antioxidant properties of domesticated and three wild ecotype forms of raspberries (Rubus idaeus L.) J. Food Sci. 2011; 76: 585–593.
17-Zamarreno, D.M.; Bustamante-Rangel, M.; Martínez - Pelarda, D. Carabias - Martínez, R. Analysis of beta - sitosterol in seeds and nuts using pressurized liquid extraction and liquid chromatography, Anal. Sci.2010 ; 25(6): 765–768
19-Lowry, OH.; Rosebrongh, N.J.; Farr AL& Randall R J,protein measurement with the folin phenol reagent. J Biol chem. 1951;193:265.
20-Tietz, N. W. Textbook of clinical chemistry. 3rded. C.A. Burtis, E.R. Ashwood, W.B. Saunders. 1999; 819-861,1245-50.
21-Sedlak, J. and Lindsay, R. H. Analytical biochemistry. 1968; 192. (Cited by Al-Zamyle2001).
22-Guidet , B. and shah, S. V. Am J. Physiol.1989; 257(26):440. (cited by Muslih, R. K.; Al-Nimer,M. S and Al-Zamely,O. Y., The level of Malondialdehyde after activation with H2O2 and CuSO4 and inhibition by deferoxamine and Molsidomine in the serum of patient with acute Myocardial infarction. National. J. chem. 2002; 5: 139-48).
25-Sharma, R.A.; Singh, B.; Singh, D. and Chandrawat , P. .Different medicinal plants and their medicinal values are widely used. J. of Medicinal Plants. 2009;3: 1153.
26-McAnalley, B.; Vennum, E.; Ramberg, J.; Koepke, C. M.; Boyd, S.; McAnalley, S. Antioxidants: Consolidated Review of Potential Benefits. 2005
28-Kampa, M.; Nistakaki, A.; Tsaousis V.; Votas, G.; Nistikaki, A.; Hatzoglou, A. and Blekas, G., Antiproliferative and apoptotic effect of selective phenolic acids on T 4 D7 human breast cancer cells. Potential of mechanism of action. Breast cancer research. 2003;6 (2): 63 – 74.
29-Tietz, N.W.; Fandamental of clinical chemistry 3th Ed.,saunderes .Toxcol Enco.2000;19(3):201-213.
30-Sies, H. Oxidative stress: introductory remarks. Sies H., ed. Oxidative stress. New York: Academic, 1995:1 – 8.
31-Mc Graw, H. Toxicology. The basic science of poisons. Casarett and Doulls. 1995.