Identify some virulence factors of bacteria Proteus mirabilis isolated from people with otitis
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Abstract
This study was carried out during the period from 5/4/ to 13/11/2016, collected (50) then swab from patients with acute and chronic otitis reviewers to Advisory clinic ear nose and throat Division at Baquba General Hospital, The aim of this current study was to isolate and diagnose proteus mirabilis and detection of some of its virulence factors. Smear incubated culture media and diffrentation, and then subjected to microscopic, biochemical tests germ farms for the purpose of diagnosing the variable spores. Test investigation of dynamic membrane configuration outside the living body run by using the variable germ isolated Congo-red dye. 10 (20%) (streak of Proteus mirabilis, type either wipes which showed the volatile race not pathogen growth was 30, (60 %), while negative smears for bacterial 10 (20%) .The results of a study showed virulence factors of bacteria the variable to 10 (100 %) isolation of her ability to produce swarming movement, 9 (90%) isolation possesses the ability to analyses human red blood cells, while 9 (90%) isolation of dynamic membrane configuration capability
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References
1-Ananthanaryan, R. and Paniker, C.K. (1988).
"Enterobacteriaceae: coliform, Proteus in the
Textbook of Microbiology".3rd dition.Orient
Longman.p:259.
2-Burall, L.S.; Harro, J.M.; Li, X.; Lockatell, C.V.;
Himpsl, S.D.; et al. (2004). Proteus mirabilis genes
that contribute to pathogenesis of urinary tract
infection: identification of 25 signature-tagged
mutants attenuated at least 100-fold. Infect. Immun.,
72(5):2922-2938.
3- Penido Nde, O.; Borni, A.; Iha, L.C.; Suguri,
V.M.; Onishi, E.; et al. (2005). Intracranial
complications of otitis media : 15 years of experience
in 33 patients.Otolaryngol. Head Neck Surg.132
(1):37-42.
4- Burgos Sunchez, A.; Menaches Guardiol, M.I.;
Gras Albert, J.R. and Talavera Sanchez, J.(2000).
Descriptive study of infections ear disease in relation
to summer.Acta Otorrinlaring Esp.51(1):19-24.
5 - Shekelle, P.; Takata, G. and Chan, L.S. (2003).
Diagnosis, natural history & late effects of otitis
media with effusion. Evidence Report/Technology
Assessment No.55.AHRQ publication. No.03-E023
.Rockville ,MD :Agency for Health Care Research
and Quality.
6-Paradise, J.L.; Rockette,H.E. and Colborn, D.K.
(1997). Otitis media in 2253pittsburgh-area infants:
prevalence and risk factors during the first two years
of life.Pediatr.99:318-333.
7-Miller,K.E. (2002). Effect of smoke exposure on
otitis media recurrence. J. American Family
Physician,66(4).
8-Hafidh, M.A.; Keogh, I.; Walsh,R.M.; Walsh,M.
and Rawluk, D. (2006). Otogenic intracranial
complications: a 7-year retrospective. Am. J.
otolaryngol. 27(6):390-395.
9- Van Hasselt,P. and Van Kregten,E (2002).
Treatment of chronic suppurative otitis media with
ofloxacin in hydroxypropyl methylcelluolose ear
drops: a clinical/bacteriological study in a rural area
of Malawi. Int. J. Pediatr. Otorhinolaryngol. 63 (1):
49-56.
10- Ako-Nai, A.K.; Oluga, F.A.; Onipede, A.O.;
Adejuyigbe, E.A. and Amusa, Y.B. (2002). The
characterization of bacterial isolates from acute otitis
media in lle-lfe, southwestern Nigeria. J. Trop.
Pediatr. 48(1):15-23.
11- Morris, P.S.; Leach, A.J.; Silberberg, P.; Mellon,
G.; Wilson, C.; et al. (2005). Otitis media in young
aboriginal children from remote communities in
northern and central Australia: a cross-sectional
survey. BMC Pediatr.5:1471-2431.
12- Mah,T.F. and O, Toole, G.A. (2001). Mechanisms
of biofilm resistance to antimicrobial agents. Trends.
Microbiol. 9:34-39.
14- Baron, E.J.; Peterson, L.R. and Finegold, S.M.
(1994). Microorganisms encountered in urinary tract
in Bailey and Scott`s Diagnostic Microbiology. 9th
ed. Mosby Company .
15- Cowan, S.T and Steel K.J (2004) . Edited and
erv.by Barrow, G.I and Felthman R.K.A., Manual for
the identification of medical Bacteria. 3 rd edition.
Dock House, the water front , Cape twon 8001, South
Africa.
16- Collee, J.G.; Fraser, A.G.; Marmian,B.P. and
Simmons, A.(1996). "Mackie and McCartney
Practical Medical Microbiology".14th ed.Chuirchill
Livingstone Inc. USA.
17- Senior,B.W. and Hughes,C. (1987). Production
and properties of hemolysin from clinical isolates of
the Proteus.J.Med.Microb.24:17-25.
18- Forbes, B.A.; Sahm, D.F. and Weissfeld, A.S.
(2002). "Bailey and Scott`s Diagnostic
Microbiology". 11th ed.Mosby, Inc.St.Louis.USA.
19- Greenwood ,D.; Slack, R. and Peutherer,J.(2000).
Medical Microbiology a guide to microbiol
infections: Pathogenesis, Immunity, Laboratory.
20- Holt, J.G.; Krieg, N.R.; Sneath, P.H.A.; Staley,
J.A.; & Williams, S.T.(1994). Bergy, s Manual Of
Derminative Bacteriology. (9th) ed. Williams &
Wilkins.
22- Kuczkowski, J.; Piatek, R. and Kur, J. (2004).
Bacterial infections in chronic otitis mediausefulness
of molecular diagnostics based on PCR
method.Otolaryngol.Pol.58(3):497-504.
23- Pajor, A,; Durko, M.; Jankowski, A.; Bartoszko-
Tyczkowska, A. and Stanczyk, R. (2006).
Bacteriological evaluation in chronic otitis media.
Otolaryngol. Pol.60(5):757-763.
24- Tong,H.H.; Wieser, J.N.; James, M.A. and
DeMaria, T.F. (2001). Effect of influenza A virus
infection on nasopharyngeal colonization and otitis
media induced by transparent or opaque phenotype
variants of Streptococccus pneumoniae in the
cinchhilla model.Infect.Immun.69: 602-606.
25- Kuczkowski, J.; Samet, A. and Brzoznowski,W.
(2000). Bacteriologic evaluation of otitis externa and
chronic otitis media. Otolaryngol.Pol.54 (5):551-556.
26- Liaw, S.J.; Lai, H.C.; Ho,S.W. and Wang, W.B.
(2000). Inhibition of virulence factor expression and
swarming differentiation in Proteus mirabilis by pnitrophenylglycerol.
J. Med. Microbiol. 49:725-731.
30- Ahmed, A.A.; Osman, H.; Mansour, A.M.; Musa,
H.A.; Ahmed, A.B.; Karrar, Z. and Hassan, H.S.
(2000). Antimicrobial ageny resistance in bacterial
isolates from patients with diarrhea and urinary tract
infection in the Sudan. Am. J. Trop. Med. Hyg.
63(5,6): 259-263
31- Al-Ouqaili, M. T. S. and Al-Kubaisy, S. H. M.
(2008). Crystalline biofilm produced by Proteus
mirabilis: an overview on their formation assays and
antimicrobial interaction. Al-Anbar. J. Med., 6(1):33-
42.
33- Pfaller, M.A.; Mujeeb, I.; Hollis, R.J.; Jones, R.N.
and Doern,G.V. (2000). Evaluation of the
discriminatory powers of the dienes test and
vibotyping as typing methods for Proteus
mirabilis.J.Clin.Microbiol.38: 1077-1080.
34- Al-Duliami, A. A.; Nauman, N. G.; Hasan, A. S.
and Al-Azawi, Z. H. (2011). Virulence factors of
Proteus mirabilis isolated from patients Otitis media
in Baquba and its Peripheries. Diyala. J. Med.
1(1):69-75.
35- Al-Dawah Mohanad J.; Adnan H. Al-Hamadany;
Eman M. Al-Jarallah; (2015). Study of Some
Virulence Factors of Proteus mirabilis Isolated from
Urinary Stones Patients. Journal of Biology,
Agriculture and Healthcare.Vol.5, No.23.