Estimation of indole acetic acid in some local algae and study the optimal conditions for its production of cyanobacteria Gloeocapsa sp. PCC7428

Abstract

A In this study, isolates and diagnoses of some local microbial isolates were diagnosed by phenotypic and hereditary diagnosis. Indole Acetic Acid (IAA) was also evaluated in these isolates and in the presence and absence of amino acid tryptophan. The IAA is externally isolated into the middle and internal hormone And identified the best isolates for the production of the hormone and concluded that the IAA secreted from the outside can be adopted as a source of production better than the internal hormone for ease of separation and the possibility of utilization of the organism and other times of production and low production cost to dispense with the destruction of cells to remove the internal content of the Hormone as well as the superior efficacy of the hormone externally excreted on the internal hormone. The cyanobacteria showed Gloeocapsa sp. PCC7428 The best efficacy for the production of the hormone 12.682 ml / μg in a medium equipped with 0.5 L / gm tryptophan was for the sex of Chlorella sp. IFRPD 1018 8.313 ml/μg This result was confirmed when comparing the effectiveness of the hormone of these two species when they were developed in simple light reactors where there was a large and clear variation in the production of externally isolated IAA. Cyanobacteria recorded an efficiency of 109.694 ml / μg on day 12 of development, The highest level of activity in the day 14 was 38.005 ml / μg, which confirms that cyanobacteria are better and faster to produce the hormone. It is also observed that the production of the hormone by the cyanobacteria is clearly affected by changes in surrounding conditions, especially pH and concentration of tryptophan Added to the food medium and lighting agent and add The highest effect of the hormone was observed in the medium with pH = 7 followed by 8 and the highest efficacy at 14 days for both fixed farms 24.719 ml / μg and shaking 23.917 ml / μg with affinity Effectiveness of both species, although the advantage of faltering farms at pH 7 on day 7 was evident on fixed farms. The concentration of tryptophan was found to be proportional to the effectiveness of the hormone and to all the treatments during all periods of measurement and the superiority of the shaking farms 46.119 ml / μg on the constant 25.433 ml / μg within 7 days at the concentration of 4 L / gm and 14 days of development. For the light factor, continuous light treatment showed the highest hormone production on the vibratory farms in the first week only 19.637 ml / μg. The hormone activity decreased with the recording of the fixed plant more effective in light during the second and third weeks (35.241 and 25.165 ml / μg) respectively, In contrast to the continuous dark treatment in which the fixed farms exceeded the first week only 19.28 ml / μg and the hormone decreased during the other weeks with the superiority of the faltering farms in the second and third weeks, The measurement was recorded at 37.113 ml / μg at 14 days. When using different sources of nitrogen in the middle proved the susceptibility of cyanobacteria to exploit the substance Treptone partially containing tryptophan, which is the raw material for the production of the hormone IAA, making it better compared to the rest of the additives to the center so can be considered a successful tryptophan alternative to tryptophan .