Effect of alcoholic extract of rosmarinus against some type of entrobacteriaceae
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Abstract
Background : In this study the alcoholic extract of rosemary leaves was used to know their inhibition on the growth of some pathogenic bacteria : Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Proteus mirabilis in Muller agar using different concentrations of this alcoholic extract( 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml). The mean of inhibition zone were measured, and the most effective concentration was on the following bacteria Salmonella typhi , E. coli, P. aeruginosa, P. mirablis and K. pneumonia respectively, and when ever the concentration of extract was increasing , the mean of inhibition was increased .
It was concluded from the results of this study and other similar studies that the natural herbs were able to control the pathogenic microorganism which were resistant to antibiotic, in addition to the important role of these herbs to develop an effective drugs which are safe and cheap .
The aim
The main aim of this research was to find out the inhibitory effect of the alcoholic extract of this plant on the following microorganisms:
Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Proteus mirabilis.
Methods
The strains which are used in this study have been cultured on Mueller Hinton agar then alcoholic extracts of Rosmarinus at concentration 200 mg/ml were added to the agar then the inhibitory effects were considered by measuring the inhibition zone.
Results
This results shows that inhibitory effect of alcoholic extract was more effective on Salmonella typhimurium then E.coli. P.aeruginosa, P.mirabilis and K.pneumonia respectively.
High effect of the alcoholic extracts of plant Rosmarinus on the different type of bacteria investigation of presence of active compounds (Alkaloids, Glycoside, Tannins, Saponine, Resine and Flavonoids) in this plant were carried out.
Article Details
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References
1- Afify AMR, EL- Beltagi HS, Hammama AA, Sidky MM, Mostafa OFA., 2011. Distribution of trans-anethole and estragole in fennel (foenicalum Vulgare Mill) of callus induced from different seedling parts and fruits. ElectrorJ EnvironAgric food chem 10 (2 ):1897-1908
2- Kirbags, Zengin F and Kursat M; 2009. Antimicrobial Activites of Extracts of some plants. Pakistan Journal of Botany Vol, 41(4): 2067-2070
3- Borras Linaresa I, Arraez-Romana D, Herreroc M, Ibanezc E, Sequra- carreteroa Afrenandez- Gutierrez A; 2011. Comparison of different extraction procedures for the comprehensive characterization of bioactive phenolic compounds in Rosmarinus Officinalis by reversed- phase high performance liquid chromatography with diode arry detection coupledt to electrospray time-of-flight mass spectrum. J Chromatography A 1218:7682-7690.
4- Bakkali F, Averbeck S, Averbeck D, Idaomar M.2008 Biological effect of essential oils- Areview. Food Chem Toxical 46(2):446-475.
5- Brenner DJ, Krieg NR, Staley JT, Garrity GM, Boone DR, Devosp, Goode fellow M, Rainey FA and Schleifer KH. 2005. Bergeys. Manual of Systematic Bacteology, 2ndEdition, Michigan State University, Springer, East lan sing.
6- Brooks GF, Carroll KG, Butel JS, Morse SA and Mietzner TA 2010. Medical Microbiolog. Jawetz, Melnick and Adelbergs, 25th Edition, Mc Graw- Hill Companies, 213-219.
7- Tenover f, Mechanisms of antimicrobial resistance in bacteria. AmJ Med 2006; 11, (6A): S3-S10.
8- Canton R, Novais A, Valverde A, Machado E, Peixe L, Bag uero F, Coque TM, Prevalence and Spread of extended spectrum B-lactamase-producing Enterobacteriaceae in Europe. Clin. Microbial. Infect. 2008, 14: 144-153.
9- Silva ncc, fernandes A., Jr.Biological properties of medicinal plants: Areview of their antimicrobial
activity. J. Venom. Anim. Toxin trop. Dis. 2010, 16: 402 -213.
10- Anesini C. and peres C. 1993. Screeing of plant used in Aregentine folk medicine for antimicrobial activity.j. Ethnopagrm. 39:119-128.
11- Perez c, Pauli m, and Bazergue p. (1990). Antibiotic assay by the agar well diffusion method. J. Acta. Biologic. Acta medicine experimenalis. 15:115-119
12- Sikkema J, de Bont JA, Poolman B. Interaction of cyclic hydrocarbons with biological membranes. J Biol Chem. 1994;269(11):8022-8.
13- Abu-shanab H, Adwan G, abu-safiya D, Adwan K, Antibacterial activities of some plants extracts utilized in popular medicine in Palestine. Turk J boil. 2004:28:99-102
14- Weckesser S. 2007. Screening of plant extracts for antimicrobial yeasts with dermatological relevance. Phytomed; pp:508-516
15- Monika Sienkiewic Z, Monika lysakowska, Marta Pastuszka Z, Wojciech Bienias and Edward kowalezy K. the potential of use Basil and Rosemary Essential oils as Effective Antibacterial Agents. Molecules Issn, 2013,18:9334-9351
16- Ellof J N.1998.which extract should be used for the screening and isolation of antimicrobial components from plants?
J. Ethnopharmacol; 60:1-6.
17- Toubal 0, DJ ahoudi A, Henchiric, Bouazza M. Phytochemical Screening and Antimicrobial Evaluation of the Aqueous Extracts of Ammoides Verticillata, an Endemic Species. Journal of life sciences. 2012;6:243-247.
18- Sienkiewic Z.M ,Kowalczy K E, Wasiela M. Recent patents regarding essential oils and the significance of their constituents in human health and treatment. Recent Pat. Anti Infect. Drug discov. 2012, 17:133-140.
19- Bakkali F, Aver back S, Aver beck D, Idomar M. Biological effect of essential oils-Areview. Food Chem.. Toxical. 2008,46:446-47