Effect of Foeniculum vulgare and Metoclopramide on the Mammary Glands of Lactating Mus musculus
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Abstract
Medications affect lactation by affecting all stages of mammary gland development and function. In particular, medications may interfere with normal mammary gland development, the hormonal environment of the lactating mammary gland, milk secretion, and nutrient delivery to lactating mammary cells. This study aimed to determine the effect of Foeniculum vulgare and metoclopramide on mammary gland development in lactating female Mus musculus miceThe present study was conducted in the animal house of the College of Veterinary Medicine and the laboratories of the College of Science at Tikrit University during the period from 10/27/2024 to 1/27/2025, where 80 female mice were used in the experiment for 20 days and divided into four of groups as follows: The first group (control): included 20 lactating females (T1) given normal food and water. The second group (T2): included 20 lactating females given Foeniculum vulgare. The third group (T3) included 20 lactating females given 2 mg metoclopramide. The fourth group (T4) included 20 lactating females given 10% Foeniculum vulgare and 2 mg metoclopramide. Histological examination of the lactating groups showed increased lobule and alveoli size, with larger cavities than in the control group. The results showed increased mothers' weights during the dosing period, suggesting the materials used in the study were more effective than in the control group. Among all groups, the highest increase in mothers' weight was found in the group given fennel and metoclopramide. The current study concluded that the Foeniculum vulgar extract and metoclopramide had positive effects on the mammary glands by increasing their secretion. The drug had a greater effect than the extract, and the best results were observed in the group treated with the extract and the drug together.
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References
1. Biswas SK, Banerjee S, Baker GW, Kuo C-Y, Chowdhury I. The mammary gland: basic structure and molecular signaling during development. International Journal of Molecular Sciences. 2022;23(7):3883. https://doi.org/10.3390/ijms23073883
2. Kaskous S, Pfaffl MW. Milk properties and morphological characteristics of the donkey mammary gland for development of an adopted milking machine—A review. Dairy. 2022;3(2):233–47. https://doi.org/10.3390/dairy3020019
3. Uenoyama Y, Inoue N, Nakamura S, Tsukamura H. Kisspeptin neurons and estrogen–estrogen receptor α signaling: unraveling the mystery of steroid feedback system regulating mammalian reproduction. International Journal of Molecular Sciences. 2021;22(17):9229.
https://doi.org/10.3390/ijms22179229
4. Abdel-Rahman HA-R, Fathalla SI, Shawky SM. Effect of some nutraceutical on the Pregnancy and Lactational Performance of Rabbits. Journal of Current Veterinary Research. 2024;6(2):279–98.
5. Suarez-Trujillo A, Luecke S, Logan L, Bradshaw C, Stewart K, Minor R, et al. Changes in sow milk lipidome across lactation occur in fatty acyl residues of triacylglycerol and phosphatidylglycerol lipids, but not in plasma membrane phospholipids. Animal. 2021;15(8):100280. https://doi.org/10.1016/j.animal.2021.100280
6. Khan TM, Wu DBC, Dolzhenko AV. Effectiveness of fenugreek as a galactagogue: A network meta‐analysis. Phytotherapy Research. 2018;32(3):402–12. https://doi.org/10.1002/ptr.5972
7. Abdella M, Khalifah A. Effects of oral administration of Lepidium sativum, Moringa oleifera oils and aqueous extract of Vitex agnnus castus on reproductive performance and blood biochemical of Doe rabbits. Egyptian Journal of Rabbit Science. 2021;31(1):1–24.
https://dx.doi.org/10.21608/ejrs.2021.127380
8. Mbemya GT, Vieira LA, Canafistula FG, Pessoa ODL, Rodrigues APR. Reports on in vivo and in vitro contribution of medicinal plants to improve the female reproductive function. Reprodução & Climatério. 2017;32(2):109–19.
https://doi.org/10.1016/j.recli.2016.11.002
9. Badgujar SB, Patel VV, Bandivdekar AH. Foeniculum vulgare Mill: a review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology. BioMed research international. 2014;2014(1):842674.
https://doi.org/10.1155/2014/842674
10. Demir İ, Bulduk İ, Darwısh IA, Enginar H. A green approach for metoclopramide quantification in pharmaceutical products: new HPLC and spectrophotometric methods. Scientific Reports. 2024;14(1):8765. https://doi.org/10.1038/s41598-024-59149-6
11. Jabbar A, Qais A-F, Al-Sudani WM, Al-Ubaidi SR. Histochemical study of the effect of sweet seed (fennel) Foeniculum vulgare seeds on the mammary glands of female rats. Journal of Al-Nahrain University. 2014;17(2):45–53.
12. Romagnoli S. Practical use of hormones in small animal reproduction. Rev bras reprod anim. 2017;41(1):59–67.
13. Abdelal W. From the mosque to satellite broadcasting: a historical perspective of Hamas media strategy: University of Exeter (United Kingdom); 2012.
14. Al-Mukhtar K, Al-Allaf S, Al-Attar A. Microscopic preparations. University of Baghdad, Ministry of Higher Education and Scientific Research, Iraq. 1982.
15. Barkhordari Ahmadi F, Pourghorban A, Kharghani S, Rezaei Shahmirzadi A, Haghjoyan SM, Heydari O, et al. The effect of fennel and black seed, on breast milk, prolactin levels and anthropometric index in human and animal samples: a review. Journal of Pediatric Perspectives. 2020;8(3):11063–9. https://doi.org/10.22038/ijp-2020-3-37906
16. Mohanty I, Senapati M, Jena D, Behera P. Ethnoveterinary importance of herbal galactogogues-a review. Veterinary World. 2014;7(5). https://doi.org/10.14202/vetworld.2014.2014-325-336
17. Tabrizi SO, Mirghafourvand M, Seyedi R. The effect of metoclopramide on prolactin levels in breastfeeding mothers: a systematic review and meta-analysis. Int J Pediatr (Mashhad). 2017;5(10):5829–40. https://doi.org/10.22038/ijp-2017-24678-20386
18. KAUPPILA A, KIVINEN S, YLIKORKALA O. Metoclopramide increases prolactin release and milk secretion in puerperium without stimulating the secretion of thyrotropin and thyroid hormones. The Journal of Clinical Endocrinology & Metabolism. 1981;52(3):436–9.
https://doi.org/10.1210/jcem-52-3-436
19. McCALLUM RW, SOWERS JR, HERSHMAN JM, STURDEVANT RA. Metoclopramide stimulates prolactin secretion in man. The Journal of Clinical Endocrinology & Metabolism. 1976;42(6):1148–52. https://doi.org/10.1210/jcem-42-6-1148
20. Carlson HE, Briggs JE, McCallum RW. Stimulation of prolactin secretion by metoclopramide in the rat. Proceedings of the Society for Experimental Biology and Medicine. 1977;154(3):475–8. https://doi.org/10.3181/00379727-154-39697a
21. MacLeod R. Regulation of prolactin secretion. Frontiers in neuroendocrinology. 1976;4:169–94.
22. Ghasemi V, Kheirkhah M, Samani LN, Vahedi M. The effect of herbal tea containing fennel seed on breast milk sufficiency signs and growth parameters of Iranian infants. 2014.
23. Honarvar F, Tadayon M, Afshari P, Namjooyan F, Haghighi MH. The effect of foeniculum vulgare on serum prolactin level in lactating women. 2013.
24. Ali IH, Ahmed MS, Hamid AK. The role of fenugreek (Trigonella foenum-graecum), sesame (sesamum indicum), and domperidone in detecting the mammary glands of lactating white rats. Tikrit Journal of Pure Science. 2021;26(4):12–7. http://dx.doi.org/10.25130/tjps.26.2021.058