Estimation of 5`-nucleotidase activity from saliva of diabetic patients with partial purification of its isoenzymes
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Abstract
This study was performed on (60) saliva specimens, including (30) diabetic patients in addition (30) healthy saliva specimens were taken from healthy subjects as control group. The results showed a significant increase in the activity of saliva 5`-nucleotidase (5`-NT) from diabetic patients compared to healthy subjects.
This study included a partial purification of 5`-NT as well, using (35%) (NH4)2SO4 for precipitation and gel filteration by (sephadex G-75 ) which gave single peak. Then 5-NT was partially also purified by using Ion exchange chromatography ( DEAE-cellulos A-50), in which three isoenzymes were isolated with different degree of purity.
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