Determination of pyridoxine HCl by oxidative coupling using N,N-di methyl paraphenylene diamine dihydrochloride (DMPPH) presence of potassium hexacynoferrate(III) in basic medium
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Abstract
This research involves the development of spectrophotometric method for determination of pyridoxine hydrochloride by oxidative coupling reaction with N,N-dimethylparaphenylenediamine dihydrochloride (DMPPDAD ( in a basic medium (pH 11.6) in the presence of potassium ferricyanide to produce an intense green colour, soluble in water, stable product and absorbs at 650 nm. Beer's law was in the linear range 6-30 μg/ml of pyridoxine hydrochloride, the molar extinction coefficint, Sandellʼs sensitivity index and detection limit were 4.338 × 103 L. mol-1.cm-1 , 0.0474 μg.cm-2 and 0.756 μg/ml respectively. The RSD value was 0.63 - 1.41% depending on the concentration. This method was applied successfully to the determination of pyridoxine hydrochloride in pharmaceutical preparation samavite B6 tablet with recovery of not less than 99.5%.
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References
1-British Pharmacopeia in CD-ROM", 5th Ed., by
system simulation ltd., The stationary office,
London, (2005).
3-E.V.Kompantseva, A.V. Khalata, L.P. Ovcharenko
and N.V. Blagorazumnya; HPLC Analysis of a New
Granulated Preparation Containing Isoniazid and
Pyridoxine Hydrochloride; p. Chem. J., 39(8),
pp441-443, (2005).
4- R. Ekinci and C.Kadakal; Determination of seven
water-soluble vitamins in tarhana,atraditional
Turkish cereal food, by HPLC; ACTA
Chromatographica, 15,(2005).
5- R. Amidzic, J. Broboric, O. Cudina, S.
Vladimirov,; RP- HPLC Determination of vitamins
B1, B3, B6, FOLIC ACID and B12 in multivitamin
tablets; J.Serb.Chem.Soc.70(10)1229-1235, (2005).
6- C.Anyakora, I. Afolami, T. Ehianeta and F.
Onwumere; HPLC analysis of nicotinamide,
pyridoxine, riboflavin and thiamin in some
selected food products in Nigeria; African J. of P.
and P., 2(2). 029-036,(2008).
7-M.Walash, F. Belal, N. El-Enany and M. El-
Maghrabey; Simultaneous determination of
metoclopramide hydrochlorid and pyridoxine
hydrochloride in syrup using HPLC method with
fluorescence detection; J. of liq. C. & Related
Technol., 36( 4),(2013).
8-Y.Li and P.R. Brown; The optimization of HPLC
conditions for use with FTIR detection in the
analysis of B vitamins; ; J. of chromatographic Sci.,
41, (2003).
9- K.V. Rao, Y. Chaithanya, P. Raghavendhra, M.
Surendar and D. Banji; Method development and
validation of RP-HPLC method for simultaneous
estimation of rifampicin, isoniasid and pyridoxine
hydrochloride in bulk pharmaceutical Dosage
form; IJPRD, 4(08), 153-162, (2012).
10- P.F. Chatzimichalakis, V. F. Sammanidou, R.
Verpoorte, I.N. Papadoyannis; Development of a
validated HPLC method for the determination of
B-complex vitamins in pharmaceuticals and
biological fluids after solid phase extraction; J.
Sep. Sci., 27(14), 1181-8, (2004).
11- A. E. F. Presoto, M. D. G. Rios, L. B. de Almeida
- Muradian; Simultaneous high performance liquid
chromatographic analysis of vitamins B1, B2 and
B6 in royal jelly; J.Braz.Chem.Soc. 15 (1),(2004) .
12- A. N. Abdul Kadir; Spectrophotometric
determination of vitamin B6 by coupling with
diazotized p-nitroaniline; Jou. Raf. Sci., 21 (4), pp
49- 59,( 2010).
13- I. Muszalska, M. Puchalska and A. Sobczak;
Determination of vitamin B6 by means of
differential spectrophotometry in pharmaceutical
preparations in the presence of magnesium
compounds; Acta Poloniae Pharmaceutica Drug
Research, 68 (6) , 845-851, (2011).
14- A. RAZA, T. M. ANSARI, S. B. NIAZI and A.
REHMAN; Spectrophotometric determination of
pyridoxine hydrochloride (vitamin B6) in bulk and
tablets by charge-transfer complexation with
chloranil; J. of the Chemical Society of Pakistan, 29
(1), (2007).
15- M. Raeed, A. Qadir, A. Mosa;
Spectrophotometric assay of pyridoxine
hydrochloride(vitamin B6) in pharmaceutical
preparations and serum Via arsenazo III- Cerium
(III) Reaction; Raf. Jour. Sci., 19 (2), 28 - 41,
(2008).
16-A. Pathak and S. J. Rajput ; Simultaneous
derivative spectrophotometric analysis of
doxylamine succinate, pyridoxine hydrochloride
and folic acid in combined dosage forms; Indian J.
Pharm. Sci., 70(4), 513–517,( 2008).
17- M. S. Arayne, N. Sultana, F. A. Siddiqui, M H.
Zuberi, A. Z. Mirza ; Spectrophotometric methods
for the simultaneous analysis of meclezine
hydrochloride and pyridoxine hydrochloride in
bulk drug and pharmaceutical formulations;
Pakistan j. of pharma. Scie. ; 20(2),149-56,( 2007).
18- G.A. Mostafa , Sel-S. Ghazy ; Potentiometric
membrane sensors for the selective determination
of pyridoxine hydrochloride (vitamin B6) in some
pharmaceutical formulations; Ann Chim. ,93(7-
8),691-9,( 2003).
19- A. R. Pires, A. N. Araújo, J. A. Lopes and M.
Conceição; Simultaneous potentiometric
determination of thiamine and pyridoxine in
multivitamins using a single cyclodextrin-based
thiamine-selective electrode; Anal. Lette. , 42 (13),
1923-1939, (2009).
20- M. Q. Al-Abachi, S. Subhi; Flow injectionspectrophotometric
determination of salbutamol
sulphate and pyridoxine hydrochroide using 2,4-
dinitrophenyhydrazine; Iraqi J. of Scie., 54 (1) , 6-
16,( 2013).