Estimate the level of the balance of oxidants - antioxidants in plasma and semen of healthy males and local rabbits exposed to oxidative stress induced cadmium chloride and preventive role of pomegranate juice against the state of oxidative stress
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Abstract
This study was designed to estimate the level of the balance of oxidation-antioxidants in both serum and semen and histological effect to the fabric of the testes in male local rabbits Oryctolagus cuniculus health and cadmium chloride treatment, in addition to the protective role of pomegranate juice against the toxicity of cadmium chloride. The were used rabbits in this study, 20 male were randomly divided into four groups each group containing 5 animals, The first group given distilled water promised healthy control group, The second group was treated cadmium chloride 5 mg / kg of body weight and promised infected control, The third group given pomegranate juice 6 ml / kg of body weight, The fourth group was treated cadmium chloride 5 mg / kg of body weight with given pomegranate juice 6 ml / kg of body weight, with the results of the study showed that the treatment of animals cadmium chloride led to decrease significantly ( P≤ 0.05) in the total content of antioxidants level of total antioxidant Capacity (TAC) and glutathione (GSH) and enzyme Catalase (CAT), and increase the concentration of Malondialehyde (MDA) for each of the blood and semen serum compared with the control group, in addition treatment of animals led cadmium chloride to bring about changes textile negative apparent in the tissues of the testes consisted crash of the basement membrane of most tubule sperm with thickening in other tubules with the presence of small numbers of cells generating sperm and the loss of most of its formative stages, while the dosage animal treatment cadmium chloride juice pomegranate raised increase significantly the total content of antioxidants level (TAC) and glutathione and enzyme Catalase, and the decrease concentration of (MDA) serum and semen and improved tissue and clear to the fabric of the testes, compared with the treatment group cadmium chloride.
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